Cloacimonas acidaminovorans (strain Evry)

Taxonomy: cellular organisms; Bacteria; FCB group; Candidatus Cloacimonetes; Candidatus Cloacimonas; Candidatus Cloacimonas acidaminovorans

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1813 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0VJF9|B0VJF9_CLOAI Diaminopimelate decarboxylase (DAP decarboxylase) OS=Cloacimonas acidaminovorans (strain Evry) OX=459349 GN=lysA PE=4 SV=1
MM1 pKa = 7.2KK2 pKa = 9.26RR3 pKa = 11.84TYY5 pKa = 10.51IFIGILFLALCLNAQPWEE23 pKa = 4.0MDD25 pKa = 2.81NSVFNPSGVPSFSFSQPRR43 pKa = 11.84FMDD46 pKa = 4.31LDD48 pKa = 3.72SDD50 pKa = 4.08GDD52 pKa = 3.65FDD54 pKa = 4.4FWLGNTNRR62 pKa = 11.84PPLYY66 pKa = 9.99LQNNGTATNPVFAPGPDD83 pKa = 3.69LLSGIPYY90 pKa = 9.58LASEE94 pKa = 4.52IAVSADD100 pKa = 3.3MNGDD104 pKa = 4.33GILDD108 pKa = 4.18LVTGGFSGLHH118 pKa = 5.97LFLNSGTNANPVFTEE133 pKa = 3.65SGGYY137 pKa = 9.43FSGLNVGLNPVPDD150 pKa = 3.81VADD153 pKa = 3.38VDD155 pKa = 3.95NDD157 pKa = 3.54GDD159 pKa = 4.2LDD161 pKa = 4.2LVVGLSEE168 pKa = 4.9DD169 pKa = 3.65GSVIIYY175 pKa = 9.71FNTGSAAAGNFSEE188 pKa = 5.42NNCQLLGDD196 pKa = 4.08IGLYY200 pKa = 10.1AYY202 pKa = 9.49PVFCDD207 pKa = 3.91FDD209 pKa = 5.27SDD211 pKa = 3.86GKK213 pKa = 10.86QDD215 pKa = 3.57ILCGRR220 pKa = 11.84DD221 pKa = 2.9AFGFVYY227 pKa = 10.0YY228 pKa = 10.24QNIGTATNPVWEE240 pKa = 4.45EE241 pKa = 3.9NNTLFAGLGMSTYY254 pKa = 9.68WNSPDD259 pKa = 5.29LVDD262 pKa = 5.35LNGDD266 pKa = 3.64GLYY269 pKa = 11.05DD270 pKa = 3.96LVYY273 pKa = 9.45GTAAGPLQYY282 pKa = 10.28YY283 pKa = 7.13VHH285 pKa = 6.98NGTAEE290 pKa = 4.04NPSWQQNTSLFGGVLDD306 pKa = 4.75VGGASNPVFYY316 pKa = 10.95DD317 pKa = 3.46FDD319 pKa = 6.33GDD321 pKa = 3.76GDD323 pKa = 4.69LDD325 pKa = 4.86LISGNQLGYY334 pKa = 11.01VKK336 pKa = 10.15FYY338 pKa = 10.96RR339 pKa = 11.84NTGTAYY345 pKa = 10.41APAWQEE351 pKa = 3.98DD352 pKa = 3.22NSYY355 pKa = 10.38FANIHH360 pKa = 5.71HH361 pKa = 7.09SIYY364 pKa = 10.71SAVTVGDD371 pKa = 3.43VDD373 pKa = 5.07ADD375 pKa = 3.82GLPDD379 pKa = 4.86VILGDD384 pKa = 4.26LNGGLYY390 pKa = 10.25FYY392 pKa = 10.7HH393 pKa = 5.96NTGTGLIEE401 pKa = 4.02QSGVLPAVSVGGWSCPRR418 pKa = 11.84LIDD421 pKa = 3.65MDD423 pKa = 4.15FDD425 pKa = 5.43GDD427 pKa = 3.75LDD429 pKa = 3.97LVVGNEE435 pKa = 3.86AGNLFYY441 pKa = 10.84YY442 pKa = 10.09QNNGTPYY449 pKa = 9.46SPLWEE454 pKa = 4.07LVNGFFGAIDD464 pKa = 3.65VGSDD468 pKa = 3.69CVPTFADD475 pKa = 3.35VDD477 pKa = 3.93EE478 pKa = 6.36DD479 pKa = 4.9DD480 pKa = 5.28DD481 pKa = 6.69LDD483 pKa = 3.95MVTGNLFGEE492 pKa = 4.71VQCFLRR498 pKa = 11.84QRR500 pKa = 11.84QLWVEE505 pKa = 3.95NTTLFSGIEE514 pKa = 4.04TDD516 pKa = 4.06QNAAPALVDD525 pKa = 4.47LDD527 pKa = 3.99HH528 pKa = 7.58DD529 pKa = 4.59GDD531 pKa = 4.07FDD533 pKa = 5.57LVLGDD538 pKa = 3.69YY539 pKa = 11.13DD540 pKa = 3.77GTFKK544 pKa = 10.86FFRR547 pKa = 11.84NLKK550 pKa = 9.83YY551 pKa = 10.51SGSVLNPPQNPIFIIDD567 pKa = 3.92GEE569 pKa = 4.71VTVTWEE575 pKa = 3.95EE576 pKa = 3.92PLIGSTSPFEE586 pKa = 4.09HH587 pKa = 6.35YY588 pKa = 10.23KK589 pKa = 10.26IYY591 pKa = 10.64LDD593 pKa = 4.37GVFCGSTTEE602 pKa = 4.36NIWVFSGLDD611 pKa = 3.06TGVAYY616 pKa = 10.31LVSITAQYY624 pKa = 10.08VAGEE628 pKa = 4.26SLPVTLEE635 pKa = 3.99FIITGNDD642 pKa = 3.43DD643 pKa = 4.3QIVQPITLTNYY654 pKa = 7.75PNPFNPSTTISFTIPAGSKK673 pKa = 8.91GTLEE677 pKa = 3.85IFNVKK682 pKa = 8.03GQKK685 pKa = 9.28VRR687 pKa = 11.84VWNYY691 pKa = 10.28LPSGEE696 pKa = 4.4HH697 pKa = 6.1KK698 pKa = 10.55IVFNGCNEE706 pKa = 3.9QGLPLPSGVYY716 pKa = 9.53FGIMKK721 pKa = 7.74TQNRR725 pKa = 11.84VQIRR729 pKa = 11.84KK730 pKa = 7.91MALVKK735 pKa = 10.76

Molecular weight: 79.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0VGK6|B0VGK6_CLOAI Transcription-repair-coupling factor OS=Cloacimonas acidaminovorans (strain Evry) OX=459349 GN=mfd PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.3QPSNRR10 pKa = 11.84SRR12 pKa = 11.84KK13 pKa = 7.07NTHH16 pKa = 5.33GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.46NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.07GRR39 pKa = 11.84KK40 pKa = 6.31TLTVV44 pKa = 3.22

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1813

0

1813

649216

24

6457

358.1

40.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.513 ± 0.062

1.13 ± 0.022

5.034 ± 0.041

6.593 ± 0.067

4.552 ± 0.041

6.145 ± 0.055

1.677 ± 0.021

8.435 ± 0.049

6.825 ± 0.087

10.042 ± 0.086

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.247 ± 0.028

5.837 ± 0.064

4.268 ± 0.039

3.7 ± 0.035

3.884 ± 0.042

6.273 ± 0.054

5.539 ± 0.102

5.61 ± 0.055

1.157 ± 0.027

4.538 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski