Murine minute virus (strain MVM prototype) (MVM) (Murine minute virus (strain MVM(p)))
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q76W05|Q76W05_MUMIP Uncharacterized protein VP OS=Murine minute virus (strain MVM prototype) OX=648235 GN=VP PE=4 SV=1
MM1 pKa = 7.73 AGNAYY6 pKa = 10.27 SDD8 pKa = 4.01 EE9 pKa = 4.3 VLGATNWLKK18 pKa = 10.73 EE19 pKa = 3.92 KK20 pKa = 10.84 SNQEE24 pKa = 3.58 VFSFVFKK31 pKa = 11.24 NEE33 pKa = 3.39 NVQLNGKK40 pKa = 9.6 DD41 pKa = 3.49 IGWNSYY47 pKa = 10.08 KK48 pKa = 10.4 KK49 pKa = 10.12 EE50 pKa = 3.99 LQEE53 pKa = 5.82 DD54 pKa = 4.14 EE55 pKa = 5.18 LKK57 pKa = 10.86 SLQRR61 pKa = 11.84 GAEE64 pKa = 4.29 TTWDD68 pKa = 3.39 QSEE71 pKa = 4.09 DD72 pKa = 3.96 MEE74 pKa = 4.61 WEE76 pKa = 4.24 TTVDD80 pKa = 3.15 EE81 pKa = 4.3 MTKK84 pKa = 10.62 KK85 pKa = 10.64 FGTLTIHH92 pKa = 6.55 DD93 pKa = 4.13 TEE95 pKa = 5.41 KK96 pKa = 10.94 YY97 pKa = 9.93 ASQPEE102 pKa = 4.59 LCTNSTCIGSRR113 pKa = 11.84 GPGFRR118 pKa = 11.84 ALEE121 pKa = 3.97 HH122 pKa = 6.05 TKK124 pKa = 10.49 YY125 pKa = 10.51 SCCGHH130 pKa = 6.26 CRR132 pKa = 11.84 NPEE135 pKa = 3.57 HH136 pKa = 6.55 WGSWFQSLPRR146 pKa = 11.84 WSTEE150 pKa = 3.52 PNLVRR155 pKa = 11.84 DD156 pKa = 3.64 RR157 pKa = 11.84 GGFEE161 pKa = 3.84 SVLRR165 pKa = 11.84 CGTVEE170 pKa = 4.26 EE171 pKa = 4.26 RR172 pKa = 11.84 LQRR175 pKa = 11.84 AAEE178 pKa = 4.04 LGLRR182 pKa = 11.84 YY183 pKa = 10.33 DD184 pKa = 4.2 GASSS188 pKa = 3.15
Molecular weight: 21.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.036
IPC2_protein 5.118
IPC_protein 5.016
Toseland 4.94
ProMoST 5.13
Dawson 4.978
Bjellqvist 5.118
Wikipedia 4.813
Rodwell 4.902
Grimsley 4.863
Solomon 4.978
Lehninger 4.927
Nozaki 5.092
DTASelect 5.194
Thurlkill 4.94
EMBOSS 4.851
Sillero 5.181
Patrickios 3.999
IPC_peptide 4.991
IPC2_peptide 5.181
IPC2.peptide.svr19 5.154
Protein with the highest isoelectric point:
>tr|Q84367|Q84367_MUMIP VP1 protein OS=Murine minute virus (strain MVM prototype) OX=648235 GN=VP1 PE=1 SV=1
MM1 pKa = 7.68 APPAKK6 pKa = 9.63 RR7 pKa = 11.84 AKK9 pKa = 9.84 RR10 pKa = 11.84 GKK12 pKa = 9.78 GLRR15 pKa = 11.84 DD16 pKa = 3.13 GWLVGYY22 pKa = 10.16
Molecular weight: 2.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.274
IPC2_protein 10.116
IPC_protein 11.052
Toseland 11.242
ProMoST 11.242
Dawson 11.286
Bjellqvist 11.067
Wikipedia 11.564
Rodwell 11.506
Grimsley 11.316
Solomon 11.535
Lehninger 11.491
Nozaki 11.213
DTASelect 11.067
Thurlkill 11.228
EMBOSS 11.667
Sillero 11.228
Patrickios 11.33
IPC_peptide 11.55
IPC2_peptide 10.145
IPC2.peptide.svr19 8.341
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
6
11
3914
9
729
355.8
39.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.515 ± 0.47
1.788 ± 0.33
5.008 ± 0.195
6.055 ± 0.933
3.73 ± 0.144
7.971 ± 0.561
2.146 ± 0.139
3.628 ± 0.324
5.749 ± 0.867
7.128 ± 0.419
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.146 ± 0.088
6.208 ± 0.208
5.416 ± 0.532
4.829 ± 0.104
4.318 ± 0.28
6.49 ± 0.263
8.712 ± 0.223
5.851 ± 0.238
3.117 ± 0.243
3.194 ± 0.36
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here