Roseovarius nanhaiticus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3516 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N7H195|A0A1N7H195_9RHOB Transcriptional regulator TetR family OS=Roseovarius nanhaiticus OX=573024 GN=SAMN05421666_2338 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.25KK3 pKa = 9.84HH4 pKa = 6.23LLSTSAIALGTVALAAPAAAQEE26 pKa = 4.17WDD28 pKa = 3.48VSFGGFMSQHH38 pKa = 4.98VAYY41 pKa = 10.22ADD43 pKa = 3.75LSGSAVPAGSAQSLSAGDD61 pKa = 3.76FDD63 pKa = 5.7GVDD66 pKa = 2.72IHH68 pKa = 7.4SNSEE72 pKa = 4.04IIFTPSITLDD82 pKa = 3.03NGLTFGVNVQLEE94 pKa = 4.58TEE96 pKa = 4.16QNGAGEE102 pKa = 4.2IDD104 pKa = 3.82EE105 pKa = 5.03SYY107 pKa = 7.89MTISSDD113 pKa = 3.21TLGQIIIGSEE123 pKa = 3.39NSAGYY128 pKa = 10.45KK129 pKa = 10.5SMVGAPGVTSMYY141 pKa = 10.02INSPSISTFIPFSAAYY157 pKa = 9.35PGGFRR162 pKa = 11.84QAGLSSFTEE171 pKa = 4.07VAGNNDD177 pKa = 3.79VQRR180 pKa = 11.84LTYY183 pKa = 7.37FTPSFNGLTLGVSYY197 pKa = 11.2APTGNNNVANSFGVNKK213 pKa = 8.04NTPGVLSDD221 pKa = 3.61IFDD224 pKa = 3.37IGVNYY229 pKa = 10.44SQTFGTTSVTLSGRR243 pKa = 11.84YY244 pKa = 8.98GVGDD248 pKa = 3.58TNIAGVSDD256 pKa = 4.23PEE258 pKa = 3.98TWALGFQVGFADD270 pKa = 4.36FTFGGSYY277 pKa = 10.94AEE279 pKa = 4.36NDD281 pKa = 3.16NGVANGAGDD290 pKa = 3.52SSGYY294 pKa = 9.54SLGMTYY300 pKa = 10.49DD301 pKa = 3.06IAGPWAVEE309 pKa = 3.3ALAYY313 pKa = 9.83IGEE316 pKa = 4.4TDD318 pKa = 3.52VGASNADD325 pKa = 3.41YY326 pKa = 10.12EE327 pKa = 4.45AYY329 pKa = 10.39RR330 pKa = 11.84IGASRR335 pKa = 11.84DD336 pKa = 3.37LGPGVDD342 pKa = 2.92WDD344 pKa = 3.83IYY346 pKa = 10.43VVQLDD351 pKa = 3.89ADD353 pKa = 3.87NGGVGAANRR362 pKa = 11.84DD363 pKa = 3.31VSGTVIGTGINLSFF377 pKa = 4.55

Molecular weight: 38.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N7F4Y9|A0A1N7F4Y9_9RHOB Uncharacterized protein OS=Roseovarius nanhaiticus OX=573024 GN=SAMN05421666_0748 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3516

0

3516

1111941

26

3015

316.3

34.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.915 ± 0.059

0.895 ± 0.014

6.129 ± 0.036

5.81 ± 0.038

3.51 ± 0.026

8.947 ± 0.038

2.071 ± 0.021

5.337 ± 0.027

2.952 ± 0.038

9.939 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.958 ± 0.023

2.444 ± 0.022

5.104 ± 0.025

3.149 ± 0.023

6.841 ± 0.039

5.136 ± 0.027

5.376 ± 0.028

6.877 ± 0.034

1.386 ± 0.018

2.224 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski