Pedococcus cremeus
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4010 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H9TWV2|A0A1H9TWV2_9MICO Cardiolipin synthase OS=Pedococcus cremeus OX=587636 GN=SAMN05216199_1789 PE=3 SV=1
MM1 pKa = 7.68 SEE3 pKa = 4.26 LEE5 pKa = 4.08 YY6 pKa = 10.75 PEE8 pKa = 4.46 DD9 pKa = 3.33 LRR11 pKa = 11.84 YY12 pKa = 7.8 TAEE15 pKa = 4.25 HH16 pKa = 5.63 EE17 pKa = 4.27 WVRR20 pKa = 11.84 TGDD23 pKa = 3.53 GGVVRR28 pKa = 11.84 VGITSFAQDD37 pKa = 3.07 ALGDD41 pKa = 3.82 VVYY44 pKa = 11.18 VSLPTVGDD52 pKa = 3.62 TVAAGDD58 pKa = 3.41 ACGEE62 pKa = 4.23 VEE64 pKa = 4.37 STKK67 pKa = 10.7 SVSDD71 pKa = 4.31 LYY73 pKa = 11.48 SPLAGEE79 pKa = 4.13 VTAVNEE85 pKa = 4.31 ALDD88 pKa = 3.89 ATPEE92 pKa = 4.05 LVNTDD97 pKa = 3.69 PYY99 pKa = 11.34 GEE101 pKa = 3.76 GWMYY105 pKa = 10.18 EE106 pKa = 4.23 LKK108 pKa = 10.61 VDD110 pKa = 4.46 DD111 pKa = 4.48 LAAVEE116 pKa = 4.69 ALMDD120 pKa = 3.4 QAAYY124 pKa = 10.47 RR125 pKa = 11.84 EE126 pKa = 4.17 QLGG129 pKa = 3.45
Molecular weight: 13.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.745
IPC_protein 3.706
Toseland 3.503
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.592
Rodwell 3.528
Grimsley 3.414
Solomon 3.668
Lehninger 3.617
Nozaki 3.808
DTASelect 3.973
Thurlkill 3.554
EMBOSS 3.605
Sillero 3.821
Patrickios 0.896
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A1H9XBC6|A0A1H9XBC6_9MICO Fructose-1 6-bisphosphatase OS=Pedococcus cremeus OX=587636 GN=SAMN05216199_3607 PE=4 SV=1
MM1 pKa = 7.19 GRR3 pKa = 11.84 QVGRR7 pKa = 11.84 QVGRR11 pKa = 11.84 QVGRR15 pKa = 11.84 QVGRR19 pKa = 11.84 QVGRR23 pKa = 11.84 QMGRR27 pKa = 11.84 LARR30 pKa = 11.84 AVRR33 pKa = 11.84 LGSGG37 pKa = 3.19
Molecular weight: 4.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.502
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.398
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.149
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.179
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4010
0
4010
1306711
30
2118
325.9
34.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.402 ± 0.059
0.712 ± 0.01
5.883 ± 0.032
5.558 ± 0.046
2.757 ± 0.023
9.367 ± 0.038
2.275 ± 0.02
3.055 ± 0.027
2.052 ± 0.032
10.311 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.857 ± 0.015
1.745 ± 0.022
5.73 ± 0.031
2.952 ± 0.025
7.626 ± 0.042
5.404 ± 0.028
6.152 ± 0.037
9.633 ± 0.035
1.615 ± 0.018
1.913 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here