Gordonia phage Ghobes

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ghobesvirus; Gordonia virus Ghobes

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3B057|A0A1B3B057_9CAUD Uncharacterized protein OS=Gordonia phage Ghobes OX=1887647 GN=44 PE=4 SV=1
MM1 pKa = 7.43FEE3 pKa = 4.61ALFALVLTLAGPHH16 pKa = 5.46QAPAHH21 pKa = 5.74VEE23 pKa = 3.92VSAAASCVLDD33 pKa = 4.12TPRR36 pKa = 11.84GPAPCPPPVVSHH48 pKa = 6.56GPAAPGGGNDD58 pKa = 2.98GSTYY62 pKa = 10.68VKK64 pKa = 10.42PEE66 pKa = 3.92LPAYY70 pKa = 7.41EE71 pKa = 4.72APSFSAPAEE80 pKa = 4.14PEE82 pKa = 4.11EE83 pKa = 4.16PAEE86 pKa = 4.17PVDD89 pKa = 3.85EE90 pKa = 4.58EE91 pKa = 4.45EE92 pKa = 5.26GGVAA96 pKa = 5.08

Molecular weight: 9.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3B0B0|A0A1B3B0B0_9CAUD dUTP diphosphatase OS=Gordonia phage Ghobes OX=1887647 GN=55 PE=4 SV=1
MM1 pKa = 7.48LLVLQRR7 pKa = 11.84GNTLLEE13 pKa = 3.74TRR15 pKa = 11.84LWVLTPGKK23 pKa = 10.26VQVLVLSPLPFPDD36 pKa = 3.47GRR38 pKa = 11.84HH39 pKa = 5.31HH40 pKa = 7.18VLVCRR45 pKa = 11.84FTLAA49 pKa = 3.59

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

14541

47

1440

246.5

27.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.309 ± 0.798

0.743 ± 0.14

5.99 ± 0.232

7.001 ± 0.286

3.652 ± 0.175

8.363 ± 0.505

1.795 ± 0.205

0.626 ± 0.14

4.202 ± 0.258

10.742 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.605 ± 0.039

3.473 ± 0.267

5.364 ± 0.232

4.278 ± 0.186

6.464 ± 0.276

5.742 ± 0.283

6.052 ± 0.226

9.566 ± 0.327

2.201 ± 0.151

2.833 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski