Amino acid dipepetide frequency for Gordonia phage Ghobes

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.93AlaAla: 12.93 ± 2.492
0.481AlaCys: 0.481 ± 0.21
5.089AlaAsp: 5.089 ± 0.479
7.497AlaGlu: 7.497 ± 0.673
3.92AlaPhe: 3.92 ± 0.667
8.735AlaGly: 8.735 ± 1.334
1.994AlaHis: 1.994 ± 0.378
0.963AlaIle: 0.963 ± 0.346
5.915AlaLys: 5.915 ± 0.78
9.285AlaLeu: 9.285 ± 1.04
0.894AlaMet: 0.894 ± 0.263
3.989AlaAsn: 3.989 ± 0.702
3.645AlaPro: 3.645 ± 0.589
5.021AlaGln: 5.021 ± 0.699
6.671AlaArg: 6.671 ± 0.735
6.878AlaSer: 6.878 ± 0.945
5.708AlaThr: 5.708 ± 0.719
9.697AlaVal: 9.697 ± 0.973
1.582AlaTrp: 1.582 ± 0.301
2.201AlaTyr: 2.201 ± 0.439
0.0AlaXaa: 0.0 ± 0.0
Cys
0.55CysAla: 0.55 ± 0.204
0.069CysCys: 0.069 ± 0.071
0.688CysAsp: 0.688 ± 0.223
0.413CysGlu: 0.413 ± 0.21
0.275CysPhe: 0.275 ± 0.155
0.757CysGly: 0.757 ± 0.265
0.206CysHis: 0.206 ± 0.112
0.0CysIle: 0.0 ± 0.0
0.069CysLys: 0.069 ± 0.066
0.55CysLeu: 0.55 ± 0.203
0.069CysMet: 0.069 ± 0.065
0.275CysAsn: 0.275 ± 0.119
0.55CysPro: 0.55 ± 0.201
0.206CysGln: 0.206 ± 0.16
0.344CysArg: 0.344 ± 0.181
0.481CysSer: 0.481 ± 0.181
0.619CysThr: 0.619 ± 0.232
0.757CysVal: 0.757 ± 0.226
0.275CysTrp: 0.275 ± 0.159
0.275CysTyr: 0.275 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
5.777AspAla: 5.777 ± 0.753
0.481AspCys: 0.481 ± 0.172
3.439AspAsp: 3.439 ± 0.44
4.333AspGlu: 4.333 ± 0.767
2.751AspPhe: 2.751 ± 0.359
4.195AspGly: 4.195 ± 0.463
1.032AspHis: 1.032 ± 0.239
0.069AspIle: 0.069 ± 0.07
1.994AspLys: 1.994 ± 0.402
7.497AspLeu: 7.497 ± 0.771
0.138AspMet: 0.138 ± 0.096
1.857AspAsn: 1.857 ± 0.466
4.058AspPro: 4.058 ± 0.451
2.889AspGln: 2.889 ± 0.377
4.333AspArg: 4.333 ± 0.546
3.095AspSer: 3.095 ± 0.472
3.026AspThr: 3.026 ± 0.432
5.708AspVal: 5.708 ± 0.755
1.1AspTrp: 1.1 ± 0.268
2.132AspTyr: 2.132 ± 0.45
0.0AspXaa: 0.0 ± 0.0
Glu
7.29GluAla: 7.29 ± 0.69
0.413GluCys: 0.413 ± 0.18
3.645GluAsp: 3.645 ± 0.503
6.259GluGlu: 6.259 ± 0.807
2.545GluPhe: 2.545 ± 0.429
5.915GluGly: 5.915 ± 0.564
0.963GluHis: 0.963 ± 0.292
0.206GluIle: 0.206 ± 0.134
2.613GluLys: 2.613 ± 0.483
7.772GluLeu: 7.772 ± 0.699
0.413GluMet: 0.413 ± 0.177
2.338GluAsn: 2.338 ± 0.457
2.751GluPro: 2.751 ± 0.548
3.989GluGln: 3.989 ± 0.624
3.92GluArg: 3.92 ± 0.552
3.576GluSer: 3.576 ± 0.547
3.714GluThr: 3.714 ± 0.452
7.497GluVal: 7.497 ± 0.98
2.132GluTrp: 2.132 ± 0.435
2.063GluTyr: 2.063 ± 0.402
0.0GluXaa: 0.0 ± 0.0
Phe
4.539PheAla: 4.539 ± 0.449
0.413PheCys: 0.413 ± 0.204
3.232PheAsp: 3.232 ± 0.416
2.476PheGlu: 2.476 ± 0.541
1.169PhePhe: 1.169 ± 0.241
3.783PheGly: 3.783 ± 0.514
0.55PheHis: 0.55 ± 0.166
0.0PheIle: 0.0 ± 0.0
2.063PheLys: 2.063 ± 0.42
3.645PheLeu: 3.645 ± 0.496
0.138PheMet: 0.138 ± 0.091
1.444PheAsn: 1.444 ± 0.489
1.651PhePro: 1.651 ± 0.331
1.169PheGln: 1.169 ± 0.269
2.27PheArg: 2.27 ± 0.412
1.651PheSer: 1.651 ± 0.275
2.545PheThr: 2.545 ± 0.399
2.132PheVal: 2.132 ± 0.343
0.688PheTrp: 0.688 ± 0.227
0.963PheTyr: 0.963 ± 0.268
0.0PheXaa: 0.0 ± 0.0
Gly
6.327GlyAla: 6.327 ± 1.11
0.413GlyCys: 0.413 ± 0.187
4.746GlyAsp: 4.746 ± 0.53
4.883GlyGlu: 4.883 ± 0.616
3.783GlyPhe: 3.783 ± 0.744
7.978GlyGly: 7.978 ± 1.179
1.994GlyHis: 1.994 ± 0.448
0.825GlyIle: 0.825 ± 0.269
4.677GlyLys: 4.677 ± 0.757
8.941GlyLeu: 8.941 ± 1.496
0.481GlyMet: 0.481 ± 0.184
3.232GlyAsn: 3.232 ± 0.492
3.851GlyPro: 3.851 ± 0.58
3.508GlyGln: 3.508 ± 0.588
5.64GlyArg: 5.64 ± 0.668
5.502GlySer: 5.502 ± 0.699
4.47GlyThr: 4.47 ± 0.52
8.184GlyVal: 8.184 ± 0.662
1.994GlyTrp: 1.994 ± 0.354
2.201GlyTyr: 2.201 ± 0.392
0.0GlyXaa: 0.0 ± 0.0
His
1.513HisAla: 1.513 ± 0.382
0.344HisCys: 0.344 ± 0.15
1.307HisAsp: 1.307 ± 0.285
1.169HisGlu: 1.169 ± 0.311
0.344HisPhe: 0.344 ± 0.15
1.238HisGly: 1.238 ± 0.252
0.275HisHis: 0.275 ± 0.174
0.069HisIle: 0.069 ± 0.063
0.55HisLys: 0.55 ± 0.209
2.063HisLeu: 2.063 ± 0.44
0.0HisMet: 0.0 ± 0.0
0.481HisAsn: 0.481 ± 0.155
1.651HisPro: 1.651 ± 0.36
1.1HisGln: 1.1 ± 0.374
1.032HisArg: 1.032 ± 0.311
0.55HisSer: 0.55 ± 0.199
1.1HisThr: 1.1 ± 0.282
1.788HisVal: 1.788 ± 0.382
0.413HisTrp: 0.413 ± 0.191
0.963HisTyr: 0.963 ± 0.408
0.0HisXaa: 0.0 ± 0.0
Ile
0.275IleAla: 0.275 ± 0.145
0.0IleCys: 0.0 ± 0.0
0.55IleAsp: 0.55 ± 0.217
0.413IleGlu: 0.413 ± 0.185
0.206IlePhe: 0.206 ± 0.106
0.619IleGly: 0.619 ± 0.206
0.206IleHis: 0.206 ± 0.121
0.069IleIle: 0.069 ± 0.062
0.206IleLys: 0.206 ± 0.133
0.413IleLeu: 0.413 ± 0.223
0.0IleMet: 0.0 ± 0.0
0.138IleAsn: 0.138 ± 0.091
0.688IlePro: 0.688 ± 0.261
0.069IleGln: 0.069 ± 0.075
0.688IleArg: 0.688 ± 0.235
0.55IleSer: 0.55 ± 0.198
0.619IleThr: 0.619 ± 0.308
0.413IleVal: 0.413 ± 0.22
0.069IleTrp: 0.069 ± 0.062
0.069IleTyr: 0.069 ± 0.075
0.0IleXaa: 0.0 ± 0.0
Lys
4.539LysAla: 4.539 ± 0.659
0.206LysCys: 0.206 ± 0.13
2.407LysAsp: 2.407 ± 0.465
2.957LysGlu: 2.957 ± 0.471
1.238LysPhe: 1.238 ± 0.313
2.613LysGly: 2.613 ± 0.356
0.825LysHis: 0.825 ± 0.272
0.138LysIle: 0.138 ± 0.083
2.063LysLys: 2.063 ± 0.474
5.227LysLeu: 5.227 ± 0.615
0.688LysMet: 0.688 ± 0.235
1.651LysAsn: 1.651 ± 0.318
2.545LysPro: 2.545 ± 0.471
1.1LysGln: 1.1 ± 0.285
2.751LysArg: 2.751 ± 0.422
3.026LysSer: 3.026 ± 0.487
2.613LysThr: 2.613 ± 0.512
3.439LysVal: 3.439 ± 0.423
0.825LysTrp: 0.825 ± 0.316
1.169LysTyr: 1.169 ± 0.252
0.0LysXaa: 0.0 ± 0.0
Leu
10.66LeuAla: 10.66 ± 0.986
0.963LeuCys: 0.963 ± 0.325
6.396LeuAsp: 6.396 ± 0.825
7.428LeuGlu: 7.428 ± 0.774
3.37LeuPhe: 3.37 ± 0.456
9.285LeuGly: 9.285 ± 1.031
1.719LeuHis: 1.719 ± 0.431
0.825LeuIle: 0.825 ± 0.243
3.645LeuLys: 3.645 ± 0.487
10.591LeuLeu: 10.591 ± 1.076
0.825LeuMet: 0.825 ± 0.27
2.889LeuAsn: 2.889 ± 0.487
7.221LeuPro: 7.221 ± 0.598
5.021LeuGln: 5.021 ± 0.482
7.703LeuArg: 7.703 ± 0.599
5.777LeuSer: 5.777 ± 0.722
6.602LeuThr: 6.602 ± 0.774
11.554LeuVal: 11.554 ± 0.865
1.994LeuTrp: 1.994 ± 0.41
2.957LeuTyr: 2.957 ± 0.435
0.0LeuXaa: 0.0 ± 0.0
Met
1.1MetAla: 1.1 ± 0.268
0.0MetCys: 0.0 ± 0.0
0.481MetAsp: 0.481 ± 0.182
0.069MetGlu: 0.069 ± 0.075
0.206MetPhe: 0.206 ± 0.14
0.344MetGly: 0.344 ± 0.19
0.069MetHis: 0.069 ± 0.071
0.0MetIle: 0.0 ± 0.0
0.344MetLys: 0.344 ± 0.145
0.138MetLeu: 0.138 ± 0.116
0.0MetMet: 0.0 ± 0.0
0.413MetAsn: 0.413 ± 0.154
0.275MetPro: 0.275 ± 0.147
0.206MetGln: 0.206 ± 0.119
0.688MetArg: 0.688 ± 0.221
0.688MetSer: 0.688 ± 0.227
0.619MetThr: 0.619 ± 0.188
0.413MetVal: 0.413 ± 0.166
0.0MetTrp: 0.0 ± 0.0
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
3.92AsnAla: 3.92 ± 0.936
0.138AsnCys: 0.138 ± 0.101
1.857AsnAsp: 1.857 ± 0.354
1.719AsnGlu: 1.719 ± 0.357
1.307AsnPhe: 1.307 ± 0.276
3.714AsnGly: 3.714 ± 0.559
0.344AsnHis: 0.344 ± 0.159
0.069AsnIle: 0.069 ± 0.06
1.307AsnLys: 1.307 ± 0.28
4.058AsnLeu: 4.058 ± 0.567
0.206AsnMet: 0.206 ± 0.134
1.1AsnAsn: 1.1 ± 0.272
2.82AsnPro: 2.82 ± 0.407
1.582AsnGln: 1.582 ± 0.28
2.407AsnArg: 2.407 ± 0.372
1.926AsnSer: 1.926 ± 0.404
2.201AsnThr: 2.201 ± 0.484
2.132AsnVal: 2.132 ± 0.369
0.825AsnTrp: 0.825 ± 0.262
1.1AsnTyr: 1.1 ± 0.268
0.0AsnXaa: 0.0 ± 0.0
Pro
5.64ProAla: 5.64 ± 0.816
0.481ProCys: 0.481 ± 0.188
3.508ProAsp: 3.508 ± 0.376
5.089ProGlu: 5.089 ± 0.555
1.582ProPhe: 1.582 ± 0.381
4.195ProGly: 4.195 ± 0.633
1.169ProHis: 1.169 ± 0.286
0.413ProIle: 0.413 ± 0.166
2.613ProLys: 2.613 ± 0.421
5.777ProLeu: 5.777 ± 0.758
0.275ProMet: 0.275 ± 0.142
1.582ProAsn: 1.582 ± 0.438
3.095ProPro: 3.095 ± 0.634
2.132ProGln: 2.132 ± 0.382
1.857ProArg: 1.857 ± 0.427
2.957ProSer: 2.957 ± 0.443
3.989ProThr: 3.989 ± 0.554
4.264ProVal: 4.264 ± 0.615
1.444ProTrp: 1.444 ± 0.328
1.582ProTyr: 1.582 ± 0.31
0.0ProXaa: 0.0 ± 0.0
Gln
5.158GlnAla: 5.158 ± 0.814
0.206GlnCys: 0.206 ± 0.102
2.201GlnAsp: 2.201 ± 0.411
3.164GlnGlu: 3.164 ± 0.473
2.132GlnPhe: 2.132 ± 0.514
2.957GlnGly: 2.957 ± 0.535
0.619GlnHis: 0.619 ± 0.191
0.275GlnIle: 0.275 ± 0.146
1.857GlnLys: 1.857 ± 0.312
6.396GlnLeu: 6.396 ± 0.72
0.138GlnMet: 0.138 ± 0.089
1.1GlnAsn: 1.1 ± 0.295
1.719GlnPro: 1.719 ± 0.398
1.444GlnGln: 1.444 ± 0.446
2.957GlnArg: 2.957 ± 0.483
1.444GlnSer: 1.444 ± 0.308
2.476GlnThr: 2.476 ± 0.441
4.402GlnVal: 4.402 ± 0.561
0.894GlnTrp: 0.894 ± 0.216
1.238GlnTyr: 1.238 ± 0.345
0.0GlnXaa: 0.0 ± 0.0
Arg
6.534ArgAla: 6.534 ± 0.697
0.688ArgCys: 0.688 ± 0.256
3.232ArgAsp: 3.232 ± 0.508
4.608ArgGlu: 4.608 ± 0.621
2.82ArgPhe: 2.82 ± 0.475
4.677ArgGly: 4.677 ± 0.531
1.032ArgHis: 1.032 ± 0.225
0.757ArgIle: 0.757 ± 0.226
2.82ArgLys: 2.82 ± 0.662
6.259ArgLeu: 6.259 ± 0.636
0.481ArgMet: 0.481 ± 0.213
2.613ArgAsn: 2.613 ± 0.505
2.957ArgPro: 2.957 ± 0.494
3.301ArgGln: 3.301 ± 0.594
3.989ArgArg: 3.989 ± 0.544
3.301ArgSer: 3.301 ± 0.417
4.333ArgThr: 4.333 ± 0.484
7.084ArgVal: 7.084 ± 0.772
1.582ArgTrp: 1.582 ± 0.391
1.513ArgTyr: 1.513 ± 0.341
0.0ArgXaa: 0.0 ± 0.0
Ser
6.465SerAla: 6.465 ± 0.978
0.413SerCys: 0.413 ± 0.173
3.439SerAsp: 3.439 ± 0.468
3.095SerGlu: 3.095 ± 0.49
2.545SerPhe: 2.545 ± 0.37
5.846SerGly: 5.846 ± 0.883
0.963SerHis: 0.963 ± 0.234
0.55SerIle: 0.55 ± 0.216
1.719SerLys: 1.719 ± 0.365
6.396SerLeu: 6.396 ± 0.671
0.344SerMet: 0.344 ± 0.147
1.926SerAsn: 1.926 ± 0.358
2.957SerPro: 2.957 ± 0.433
1.651SerGln: 1.651 ± 0.317
3.026SerArg: 3.026 ± 0.436
4.264SerSer: 4.264 ± 0.689
3.301SerThr: 3.301 ± 0.414
5.365SerVal: 5.365 ± 0.738
1.376SerTrp: 1.376 ± 0.288
1.788SerTyr: 1.788 ± 0.283
0.0SerXaa: 0.0 ± 0.0
Thr
6.121ThrAla: 6.121 ± 0.794
0.138ThrCys: 0.138 ± 0.11
3.714ThrAsp: 3.714 ± 0.478
4.127ThrGlu: 4.127 ± 0.613
1.994ThrPhe: 1.994 ± 0.463
5.089ThrGly: 5.089 ± 0.578
0.963ThrHis: 0.963 ± 0.262
0.55ThrIle: 0.55 ± 0.175
2.682ThrLys: 2.682 ± 0.445
6.946ThrLeu: 6.946 ± 0.699
0.206ThrMet: 0.206 ± 0.111
2.613ThrAsn: 2.613 ± 0.414
4.264ThrPro: 4.264 ± 0.505
1.994ThrGln: 1.994 ± 0.352
3.439ThrArg: 3.439 ± 0.414
4.058ThrSer: 4.058 ± 0.742
3.301ThrThr: 3.301 ± 0.655
5.983ThrVal: 5.983 ± 0.758
1.1ThrTrp: 1.1 ± 0.288
1.238ThrTyr: 1.238 ± 0.266
0.0ThrXaa: 0.0 ± 0.0
Val
9.766ValAla: 9.766 ± 0.881
1.1ValCys: 1.1 ± 0.289
6.396ValAsp: 6.396 ± 0.629
7.153ValGlu: 7.153 ± 0.664
2.682ValPhe: 2.682 ± 0.368
7.84ValGly: 7.84 ± 0.924
2.063ValHis: 2.063 ± 0.353
0.275ValIle: 0.275 ± 0.134
3.783ValLys: 3.783 ± 0.699
9.216ValLeu: 9.216 ± 1.125
0.344ValMet: 0.344 ± 0.126
3.301ValAsn: 3.301 ± 0.546
4.264ValPro: 4.264 ± 0.482
4.264ValGln: 4.264 ± 0.503
6.465ValArg: 6.465 ± 0.72
4.883ValSer: 4.883 ± 0.54
6.602ValThr: 6.602 ± 0.847
10.179ValVal: 10.179 ± 0.82
1.926ValTrp: 1.926 ± 0.393
3.164ValTyr: 3.164 ± 0.629
0.0ValXaa: 0.0 ± 0.0
Trp
1.926TrpAla: 1.926 ± 0.312
0.206TrpCys: 0.206 ± 0.119
1.376TrpAsp: 1.376 ± 0.295
1.238TrpGlu: 1.238 ± 0.302
0.894TrpPhe: 0.894 ± 0.286
1.376TrpGly: 1.376 ± 0.362
0.344TrpHis: 0.344 ± 0.174
0.206TrpIle: 0.206 ± 0.107
0.413TrpLys: 0.413 ± 0.142
3.164TrpLeu: 3.164 ± 0.49
0.138TrpMet: 0.138 ± 0.093
1.169TrpAsn: 1.169 ± 0.282
0.894TrpPro: 0.894 ± 0.214
0.619TrpGln: 0.619 ± 0.24
1.582TrpArg: 1.582 ± 0.346
1.307TrpSer: 1.307 ± 0.286
0.963TrpThr: 0.963 ± 0.243
2.751TrpVal: 2.751 ± 0.552
0.688TrpTrp: 0.688 ± 0.22
0.757TrpTyr: 0.757 ± 0.188
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.063TyrAla: 2.063 ± 0.395
0.275TyrCys: 0.275 ± 0.141
2.201TyrAsp: 2.201 ± 0.385
1.926TyrGlu: 1.926 ± 0.421
0.757TyrPhe: 0.757 ± 0.231
2.476TyrGly: 2.476 ± 0.415
0.619TyrHis: 0.619 ± 0.226
0.0TyrIle: 0.0 ± 0.0
0.688TyrLys: 0.688 ± 0.181
3.095TyrLeu: 3.095 ± 0.471
0.206TyrMet: 0.206 ± 0.127
0.619TyrAsn: 0.619 ± 0.149
1.788TyrPro: 1.788 ± 0.29
1.513TyrGln: 1.513 ± 0.348
2.889TyrArg: 2.889 ± 0.522
1.513TyrSer: 1.513 ± 0.292
1.719TyrThr: 1.719 ± 0.347
1.926TyrVal: 1.926 ± 0.529
1.1TyrTrp: 1.1 ± 0.312
0.963TyrTyr: 0.963 ± 0.307
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (14541 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski