Bacteriophage Titan-X

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pradovirus; unclassified Pradovirus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2UAI8|A0A5Q2UAI8_9CAUD Uncharacterized protein OS=Bacteriophage Titan-X OX=2662140 PE=4 SV=1
MM1 pKa = 7.62AVPDD5 pKa = 4.03PQLRR9 pKa = 11.84YY10 pKa = 8.97ATDD13 pKa = 3.21VFDD16 pKa = 5.98ANGTTTDD23 pKa = 3.16WQISFTGGYY32 pKa = 9.41INPSHH37 pKa = 6.9VYY39 pKa = 10.33AMSGILDD46 pKa = 4.18EE47 pKa = 4.42EE48 pKa = 4.69TQLLTDD54 pKa = 4.31RR55 pKa = 11.84TPHH58 pKa = 4.77TVEE61 pKa = 4.47VLSEE65 pKa = 4.73DD66 pKa = 3.71YY67 pKa = 10.79DD68 pKa = 4.14ASTVRR73 pKa = 11.84VSPAVAAGRR82 pKa = 11.84KK83 pKa = 9.05LYY85 pKa = 9.94IYY87 pKa = 10.25RR88 pKa = 11.84STPVQQMLVDD98 pKa = 3.85YY99 pKa = 10.22VNGSIISKK107 pKa = 10.87ANLNLSNDD115 pKa = 3.5QLLKK119 pKa = 10.39IIQEE123 pKa = 4.34MFDD126 pKa = 3.67SLNIATLSIDD136 pKa = 3.35QQVGVIVDD144 pKa = 4.16LNEE147 pKa = 4.43IIQNIYY153 pKa = 10.54KK154 pKa = 10.28EE155 pKa = 4.39VIQLVEE161 pKa = 3.92AGGIVSVAPRR171 pKa = 11.84VWSGAWVGEE180 pKa = 4.21DD181 pKa = 3.96AGRR184 pKa = 11.84TDD186 pKa = 4.02FDD188 pKa = 3.73MVGADD193 pKa = 3.27VSGAGFYY200 pKa = 10.67DD201 pKa = 4.08VYY203 pKa = 11.61VNGIGMQPDD212 pKa = 2.79VDD214 pKa = 4.18YY215 pKa = 11.27QVTLADD221 pKa = 3.63GTDD224 pKa = 3.34PSFIRR229 pKa = 11.84FAEE232 pKa = 4.52VPAEE236 pKa = 3.89GSIWFAVLRR245 pKa = 11.84GYY247 pKa = 10.59AKK249 pKa = 10.05PYY251 pKa = 9.17TGPTPITRR259 pKa = 11.84VSLRR263 pKa = 11.84VPVIEE268 pKa = 4.05AAGPSFYY275 pKa = 10.99ADD277 pKa = 3.4YY278 pKa = 10.82ASEE281 pKa = 4.21YY282 pKa = 11.0GLIRR286 pKa = 11.84CTYY289 pKa = 9.4ATGCNATINAIPAVGNSEE307 pKa = 4.32SKK309 pKa = 10.8LGAGSYY315 pKa = 10.82FSFQQKK321 pKa = 10.35AGVVNIVADD330 pKa = 3.78AGVTLEE336 pKa = 4.72IPAGCIARR344 pKa = 11.84TRR346 pKa = 11.84GTNSVVTATCIDD358 pKa = 3.65GDD360 pKa = 4.23TNTWLLSGDD369 pKa = 3.9LAKK372 pKa = 10.54EE373 pKa = 3.9

Molecular weight: 40.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2UAH3|A0A5Q2UAH3_9CAUD Exonuclease OS=Bacteriophage Titan-X OX=2662140 PE=4 SV=1
MM1 pKa = 7.55TNRR4 pKa = 11.84KK5 pKa = 9.35KK6 pKa = 10.57LDD8 pKa = 3.06RR9 pKa = 11.84WLMVSDD15 pKa = 3.62IAEE18 pKa = 4.28LLWWSLARR26 pKa = 11.84VFRR29 pKa = 4.77

Molecular weight: 3.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

13455

29

1633

274.6

30.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.769 ± 0.721

0.795 ± 0.154

6.228 ± 0.151

5.775 ± 0.231

3.3 ± 0.154

8.25 ± 0.348

1.88 ± 0.215

4.378 ± 0.171

5.121 ± 0.329

8.027 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.809 ± 0.201

4.37 ± 0.203

4.132 ± 0.293

4.37 ± 0.266

6.042 ± 0.298

5.678 ± 0.284

6.065 ± 0.316

6.919 ± 0.328

1.702 ± 0.11

3.389 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski