Amino acid dipepetide frequency for Bacteriophage Titan-X

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.048AlaAla: 14.048 ± 1.864
0.595AlaCys: 0.595 ± 0.205
5.946AlaAsp: 5.946 ± 0.617
7.581AlaGlu: 7.581 ± 0.744
2.824AlaPhe: 2.824 ± 0.458
7.953AlaGly: 7.953 ± 1.26
1.784AlaHis: 1.784 ± 0.295
4.608AlaIle: 4.608 ± 0.44
5.649AlaLys: 5.649 ± 0.683
7.804AlaLeu: 7.804 ± 0.763
3.865AlaMet: 3.865 ± 0.737
4.014AlaAsn: 4.014 ± 0.572
4.906AlaPro: 4.906 ± 1.252
5.203AlaGln: 5.203 ± 0.759
6.169AlaArg: 6.169 ± 0.719
5.798AlaSer: 5.798 ± 0.794
6.243AlaThr: 6.243 ± 0.654
7.358AlaVal: 7.358 ± 0.701
2.378AlaTrp: 2.378 ± 0.436
2.973AlaTyr: 2.973 ± 0.464
0.0AlaXaa: 0.0 ± 0.0
Cys
0.595CysAla: 0.595 ± 0.208
0.074CysCys: 0.074 ± 0.071
0.52CysAsp: 0.52 ± 0.152
0.446CysGlu: 0.446 ± 0.244
0.223CysPhe: 0.223 ± 0.134
0.743CysGly: 0.743 ± 0.348
0.074CysHis: 0.074 ± 0.065
0.595CysIle: 0.595 ± 0.231
0.149CysLys: 0.149 ± 0.094
0.595CysLeu: 0.595 ± 0.24
0.297CysMet: 0.297 ± 0.181
0.595CysAsn: 0.595 ± 0.208
0.297CysPro: 0.297 ± 0.154
0.372CysGln: 0.372 ± 0.244
0.297CysArg: 0.297 ± 0.155
0.595CysSer: 0.595 ± 0.256
0.743CysThr: 0.743 ± 0.25
0.372CysVal: 0.372 ± 0.163
0.0CysTrp: 0.0 ± 0.0
0.372CysTyr: 0.372 ± 0.157
0.0CysXaa: 0.0 ± 0.0
Asp
6.318AspAla: 6.318 ± 0.55
0.595AspCys: 0.595 ± 0.199
4.162AspAsp: 4.162 ± 0.515
3.939AspGlu: 3.939 ± 0.469
3.047AspPhe: 3.047 ± 0.468
5.129AspGly: 5.129 ± 0.594
0.818AspHis: 0.818 ± 0.293
3.568AspIle: 3.568 ± 0.407
3.047AspLys: 3.047 ± 0.377
4.088AspLeu: 4.088 ± 0.552
0.892AspMet: 0.892 ± 0.251
3.27AspAsn: 3.27 ± 0.47
2.676AspPro: 2.676 ± 0.4
2.601AspGln: 2.601 ± 0.365
3.642AspArg: 3.642 ± 0.586
3.047AspSer: 3.047 ± 0.434
3.345AspThr: 3.345 ± 0.406
4.831AspVal: 4.831 ± 0.731
1.041AspTrp: 1.041 ± 0.235
2.155AspTyr: 2.155 ± 0.531
0.0AspXaa: 0.0 ± 0.0
Glu
6.021GluAla: 6.021 ± 0.628
0.372GluCys: 0.372 ± 0.156
2.75GluAsp: 2.75 ± 0.376
3.493GluGlu: 3.493 ± 0.527
2.453GluPhe: 2.453 ± 0.491
5.203GluGly: 5.203 ± 0.572
1.635GluHis: 1.635 ± 0.341
2.007GluIle: 2.007 ± 0.356
2.75GluLys: 2.75 ± 0.526
6.169GluLeu: 6.169 ± 0.826
1.784GluMet: 1.784 ± 0.324
2.453GluAsn: 2.453 ± 0.464
1.858GluPro: 1.858 ± 0.438
2.899GluGln: 2.899 ± 0.456
3.27GluArg: 3.27 ± 0.583
2.676GluSer: 2.676 ± 0.476
2.304GluThr: 2.304 ± 0.329
3.716GluVal: 3.716 ± 0.577
1.264GluTrp: 1.264 ± 0.345
2.676GluTyr: 2.676 ± 0.404
0.0GluXaa: 0.0 ± 0.0
Phe
3.791PheAla: 3.791 ± 0.461
0.446PheCys: 0.446 ± 0.179
2.676PheAsp: 2.676 ± 0.476
1.561PheGlu: 1.561 ± 0.333
0.595PhePhe: 0.595 ± 0.198
2.824PheGly: 2.824 ± 0.387
0.669PheHis: 0.669 ± 0.229
1.561PheIle: 1.561 ± 0.343
1.487PheLys: 1.487 ± 0.374
2.527PheLeu: 2.527 ± 0.45
0.52PheMet: 0.52 ± 0.161
1.561PheAsn: 1.561 ± 0.379
1.71PhePro: 1.71 ± 0.386
1.115PheGln: 1.115 ± 0.266
1.561PheArg: 1.561 ± 0.294
2.081PheSer: 2.081 ± 0.338
1.933PheThr: 1.933 ± 0.443
2.23PheVal: 2.23 ± 0.569
0.223PheTrp: 0.223 ± 0.116
1.933PheTyr: 1.933 ± 0.326
0.0PheXaa: 0.0 ± 0.0
Gly
8.845GlyAla: 8.845 ± 1.312
0.818GlyCys: 0.818 ± 0.313
5.649GlyAsp: 5.649 ± 0.679
4.237GlyGlu: 4.237 ± 0.537
2.75GlyPhe: 2.75 ± 0.377
7.73GlyGly: 7.73 ± 0.811
0.818GlyHis: 0.818 ± 0.33
3.568GlyIle: 3.568 ± 0.645
4.906GlyLys: 4.906 ± 0.688
5.352GlyLeu: 5.352 ± 0.473
2.899GlyMet: 2.899 ± 0.414
3.568GlyAsn: 3.568 ± 0.441
2.304GlyPro: 2.304 ± 0.463
3.642GlyGln: 3.642 ± 0.582
4.98GlyArg: 4.98 ± 0.451
4.757GlySer: 4.757 ± 0.54
5.426GlyThr: 5.426 ± 0.74
5.872GlyVal: 5.872 ± 0.554
1.561GlyTrp: 1.561 ± 0.357
2.824GlyTyr: 2.824 ± 0.41
0.0GlyXaa: 0.0 ± 0.0
His
1.412HisAla: 1.412 ± 0.278
0.223HisCys: 0.223 ± 0.123
1.487HisAsp: 1.487 ± 0.452
1.041HisGlu: 1.041 ± 0.304
0.52HisPhe: 0.52 ± 0.192
1.487HisGly: 1.487 ± 0.352
0.52HisHis: 0.52 ± 0.191
1.041HisIle: 1.041 ± 0.267
1.338HisLys: 1.338 ± 0.475
1.041HisLeu: 1.041 ± 0.36
0.595HisMet: 0.595 ± 0.241
0.595HisAsn: 0.595 ± 0.189
1.115HisPro: 1.115 ± 0.33
0.892HisGln: 0.892 ± 0.397
1.264HisArg: 1.264 ± 0.343
0.966HisSer: 0.966 ± 0.292
0.966HisThr: 0.966 ± 0.242
1.264HisVal: 1.264 ± 0.431
0.446HisTrp: 0.446 ± 0.181
0.595HisTyr: 0.595 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
5.5IleAla: 5.5 ± 0.704
0.372IleCys: 0.372 ± 0.163
3.196IleAsp: 3.196 ± 0.345
3.047IleGlu: 3.047 ± 0.475
1.115IlePhe: 1.115 ± 0.299
3.419IleGly: 3.419 ± 0.409
1.041IleHis: 1.041 ± 0.27
1.858IleIle: 1.858 ± 0.438
2.23IleLys: 2.23 ± 0.433
2.824IleLeu: 2.824 ± 0.445
1.264IleMet: 1.264 ± 0.342
2.23IleAsn: 2.23 ± 0.439
2.824IlePro: 2.824 ± 0.47
1.635IleGln: 1.635 ± 0.324
2.453IleArg: 2.453 ± 0.272
1.858IleSer: 1.858 ± 0.369
2.676IleThr: 2.676 ± 0.417
2.824IleVal: 2.824 ± 0.539
0.372IleTrp: 0.372 ± 0.167
1.041IleTyr: 1.041 ± 0.259
0.0IleXaa: 0.0 ± 0.0
Lys
5.649LysAla: 5.649 ± 0.668
0.223LysCys: 0.223 ± 0.119
2.899LysAsp: 2.899 ± 0.437
3.419LysGlu: 3.419 ± 0.474
1.635LysPhe: 1.635 ± 0.257
3.865LysGly: 3.865 ± 0.665
1.189LysHis: 1.189 ± 0.312
1.784LysIle: 1.784 ± 0.473
2.155LysLys: 2.155 ± 0.468
5.798LysLeu: 5.798 ± 0.835
1.041LysMet: 1.041 ± 0.333
2.23LysAsn: 2.23 ± 0.419
2.601LysPro: 2.601 ± 0.509
2.304LysGln: 2.304 ± 0.43
4.088LysArg: 4.088 ± 0.767
1.784LysSer: 1.784 ± 0.336
2.527LysThr: 2.527 ± 0.462
2.601LysVal: 2.601 ± 0.454
1.264LysTrp: 1.264 ± 0.296
2.155LysTyr: 2.155 ± 0.402
0.0LysXaa: 0.0 ± 0.0
Leu
7.433LeuAla: 7.433 ± 0.855
1.041LeuCys: 1.041 ± 0.251
5.798LeuAsp: 5.798 ± 0.58
4.683LeuGlu: 4.683 ± 0.54
1.71LeuPhe: 1.71 ± 0.397
5.575LeuGly: 5.575 ± 0.578
2.081LeuHis: 2.081 ± 0.406
2.824LeuIle: 2.824 ± 0.354
4.162LeuLys: 4.162 ± 0.648
6.764LeuLeu: 6.764 ± 0.621
2.378LeuMet: 2.378 ± 0.327
4.162LeuAsn: 4.162 ± 0.517
3.939LeuPro: 3.939 ± 0.567
3.196LeuGln: 3.196 ± 0.573
6.021LeuArg: 6.021 ± 0.655
5.129LeuSer: 5.129 ± 0.646
5.277LeuThr: 5.277 ± 0.458
4.906LeuVal: 4.906 ± 0.678
1.115LeuTrp: 1.115 ± 0.336
2.081LeuTyr: 2.081 ± 0.454
0.0LeuXaa: 0.0 ± 0.0
Met
2.973MetAla: 2.973 ± 0.396
0.074MetCys: 0.074 ± 0.076
2.304MetAsp: 2.304 ± 0.437
1.71MetGlu: 1.71 ± 0.383
0.892MetPhe: 0.892 ± 0.239
1.933MetGly: 1.933 ± 0.419
0.297MetHis: 0.297 ± 0.163
0.966MetIle: 0.966 ± 0.203
1.115MetLys: 1.115 ± 0.379
2.527MetLeu: 2.527 ± 0.394
0.595MetMet: 0.595 ± 0.225
0.372MetAsn: 0.372 ± 0.156
1.189MetPro: 1.189 ± 0.425
1.71MetGln: 1.71 ± 0.331
2.23MetArg: 2.23 ± 0.609
2.75MetSer: 2.75 ± 0.284
1.858MetThr: 1.858 ± 0.426
1.635MetVal: 1.635 ± 0.342
0.074MetTrp: 0.074 ± 0.068
0.892MetTyr: 0.892 ± 0.253
0.0MetXaa: 0.0 ± 0.0
Asn
4.757AsnAla: 4.757 ± 0.698
0.149AsnCys: 0.149 ± 0.109
2.378AsnAsp: 2.378 ± 0.34
2.007AsnGlu: 2.007 ± 0.33
1.487AsnPhe: 1.487 ± 0.382
4.385AsnGly: 4.385 ± 0.635
0.669AsnHis: 0.669 ± 0.232
1.635AsnIle: 1.635 ± 0.313
2.676AsnLys: 2.676 ± 0.454
3.122AsnLeu: 3.122 ± 0.542
1.338AsnMet: 1.338 ± 0.333
1.933AsnAsn: 1.933 ± 0.363
2.304AsnPro: 2.304 ± 0.338
1.412AsnGln: 1.412 ± 0.392
2.601AsnArg: 2.601 ± 0.575
2.824AsnSer: 2.824 ± 0.405
2.899AsnThr: 2.899 ± 0.604
2.378AsnVal: 2.378 ± 0.424
0.892AsnTrp: 0.892 ± 0.179
1.858AsnTyr: 1.858 ± 0.37
0.0AsnXaa: 0.0 ± 0.0
Pro
4.46ProAla: 4.46 ± 1.231
0.372ProCys: 0.372 ± 0.176
2.973ProAsp: 2.973 ± 0.424
2.899ProGlu: 2.899 ± 0.596
1.041ProPhe: 1.041 ± 0.275
3.27ProGly: 3.27 ± 0.634
0.892ProHis: 0.892 ± 0.34
1.115ProIle: 1.115 ± 0.262
2.23ProLys: 2.23 ± 0.427
3.047ProLeu: 3.047 ± 0.448
1.264ProMet: 1.264 ± 0.279
2.155ProAsn: 2.155 ± 0.343
1.487ProPro: 1.487 ± 0.386
1.561ProGln: 1.561 ± 0.292
1.487ProArg: 1.487 ± 0.233
2.973ProSer: 2.973 ± 0.356
2.304ProThr: 2.304 ± 0.434
4.162ProVal: 4.162 ± 0.777
0.669ProTrp: 0.669 ± 0.213
0.966ProTyr: 0.966 ± 0.323
0.0ProXaa: 0.0 ± 0.0
Gln
5.798GlnAla: 5.798 ± 0.849
0.297GlnCys: 0.297 ± 0.164
1.784GlnAsp: 1.784 ± 0.325
1.933GlnGlu: 1.933 ± 0.445
1.784GlnPhe: 1.784 ± 0.313
3.493GlnGly: 3.493 ± 0.474
0.52GlnHis: 0.52 ± 0.205
1.412GlnIle: 1.412 ± 0.263
1.71GlnLys: 1.71 ± 0.274
4.46GlnLeu: 4.46 ± 0.539
1.561GlnMet: 1.561 ± 0.328
1.487GlnAsn: 1.487 ± 0.397
1.115GlnPro: 1.115 ± 0.386
2.676GlnGln: 2.676 ± 0.763
2.824GlnArg: 2.824 ± 0.562
2.676GlnSer: 2.676 ± 0.35
3.196GlnThr: 3.196 ± 0.633
2.824GlnVal: 2.824 ± 0.463
0.818GlnTrp: 0.818 ± 0.268
1.338GlnTyr: 1.338 ± 0.306
0.0GlnXaa: 0.0 ± 0.0
Arg
6.392ArgAla: 6.392 ± 0.774
0.892ArgCys: 0.892 ± 0.311
3.345ArgAsp: 3.345 ± 0.537
3.493ArgGlu: 3.493 ± 0.571
1.933ArgPhe: 1.933 ± 0.337
4.311ArgGly: 4.311 ± 0.517
1.858ArgHis: 1.858 ± 0.371
2.824ArgIle: 2.824 ± 0.616
4.46ArgLys: 4.46 ± 0.608
4.831ArgLeu: 4.831 ± 0.591
1.933ArgMet: 1.933 ± 0.342
2.75ArgAsn: 2.75 ± 0.4
1.412ArgPro: 1.412 ± 0.288
2.304ArgGln: 2.304 ± 0.463
2.973ArgArg: 2.973 ± 0.611
2.973ArgSer: 2.973 ± 0.372
3.791ArgThr: 3.791 ± 0.436
5.129ArgVal: 5.129 ± 0.66
0.818ArgTrp: 0.818 ± 0.201
2.007ArgTyr: 2.007 ± 0.402
0.0ArgXaa: 0.0 ± 0.0
Ser
6.615SerAla: 6.615 ± 0.791
0.372SerCys: 0.372 ± 0.178
3.122SerAsp: 3.122 ± 0.4
3.196SerGlu: 3.196 ± 0.359
2.23SerPhe: 2.23 ± 0.488
4.831SerGly: 4.831 ± 0.724
0.966SerHis: 0.966 ± 0.226
3.716SerIle: 3.716 ± 0.452
2.527SerLys: 2.527 ± 0.438
4.757SerLeu: 4.757 ± 0.461
1.561SerMet: 1.561 ± 0.368
1.933SerAsn: 1.933 ± 0.348
1.635SerPro: 1.635 ± 0.247
2.676SerGln: 2.676 ± 0.426
3.196SerArg: 3.196 ± 0.474
3.568SerSer: 3.568 ± 0.636
3.122SerThr: 3.122 ± 0.532
4.46SerVal: 4.46 ± 0.874
0.966SerTrp: 0.966 ± 0.292
1.338SerTyr: 1.338 ± 0.324
0.0SerXaa: 0.0 ± 0.0
Thr
6.689ThrAla: 6.689 ± 0.885
0.297ThrCys: 0.297 ± 0.164
3.716ThrAsp: 3.716 ± 0.518
3.047ThrGlu: 3.047 ± 0.63
1.635ThrPhe: 1.635 ± 0.292
6.615ThrGly: 6.615 ± 0.616
0.818ThrHis: 0.818 ± 0.243
2.453ThrIle: 2.453 ± 0.289
3.196ThrLys: 3.196 ± 0.539
4.162ThrLeu: 4.162 ± 0.475
1.189ThrMet: 1.189 ± 0.367
2.378ThrAsn: 2.378 ± 0.339
2.453ThrPro: 2.453 ± 0.385
2.973ThrGln: 2.973 ± 0.449
3.419ThrArg: 3.419 ± 0.486
3.865ThrSer: 3.865 ± 0.749
4.162ThrThr: 4.162 ± 0.513
3.791ThrVal: 3.791 ± 0.658
0.892ThrTrp: 0.892 ± 0.23
2.899ThrTyr: 2.899 ± 0.534
0.0ThrXaa: 0.0 ± 0.0
Val
6.095ValAla: 6.095 ± 0.839
0.223ValCys: 0.223 ± 0.141
4.311ValAsp: 4.311 ± 0.594
3.047ValGlu: 3.047 ± 0.456
2.676ValPhe: 2.676 ± 0.325
5.872ValGly: 5.872 ± 0.692
1.264ValHis: 1.264 ± 0.405
3.642ValIle: 3.642 ± 0.491
2.973ValLys: 2.973 ± 0.56
5.575ValLeu: 5.575 ± 0.607
1.71ValMet: 1.71 ± 0.417
3.493ValAsn: 3.493 ± 0.715
4.014ValPro: 4.014 ± 0.521
2.75ValGln: 2.75 ± 0.329
4.385ValArg: 4.385 ± 0.665
3.493ValSer: 3.493 ± 0.58
4.98ValThr: 4.98 ± 0.622
5.203ValVal: 5.203 ± 0.807
1.189ValTrp: 1.189 ± 0.321
2.304ValTyr: 2.304 ± 0.478
0.0ValXaa: 0.0 ± 0.0
Trp
0.966TrpAla: 0.966 ± 0.308
0.372TrpCys: 0.372 ± 0.153
0.446TrpAsp: 0.446 ± 0.221
0.966TrpGlu: 0.966 ± 0.198
1.041TrpPhe: 1.041 ± 0.282
0.743TrpGly: 0.743 ± 0.259
0.52TrpHis: 0.52 ± 0.186
0.818TrpIle: 0.818 ± 0.233
1.338TrpLys: 1.338 ± 0.269
2.081TrpLeu: 2.081 ± 0.399
0.818TrpMet: 0.818 ± 0.364
0.892TrpAsn: 0.892 ± 0.242
0.297TrpPro: 0.297 ± 0.152
0.52TrpGln: 0.52 ± 0.14
1.115TrpArg: 1.115 ± 0.261
1.041TrpSer: 1.041 ± 0.225
0.669TrpThr: 0.669 ± 0.214
1.264TrpVal: 1.264 ± 0.29
0.372TrpTrp: 0.372 ± 0.164
0.743TrpTyr: 0.743 ± 0.192
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.345TyrAla: 3.345 ± 0.622
0.149TyrCys: 0.149 ± 0.096
2.824TyrAsp: 2.824 ± 0.46
2.007TyrGlu: 2.007 ± 0.406
1.412TyrPhe: 1.412 ± 0.364
3.122TyrGly: 3.122 ± 0.364
0.372TyrHis: 0.372 ± 0.195
1.784TyrIle: 1.784 ± 0.547
1.115TyrLys: 1.115 ± 0.265
2.75TyrLeu: 2.75 ± 0.403
0.52TyrMet: 0.52 ± 0.246
1.635TyrAsn: 1.635 ± 0.387
1.189TyrPro: 1.189 ± 0.244
1.264TyrGln: 1.264 ± 0.291
2.453TyrArg: 2.453 ± 0.322
1.933TyrSer: 1.933 ± 0.539
2.23TyrThr: 2.23 ± 0.484
2.378TyrVal: 2.378 ± 0.504
0.669TyrTrp: 0.669 ± 0.226
0.743TyrTyr: 0.743 ± 0.244
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (13455 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski