Candidatus Thioglobus autotrophicus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; Candidatus Thioglobus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1488 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M4NGT5|A0A0M4NGT5_9GAMM 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase OS=Candidatus Thioglobus autotrophicus OX=1705394 GN=SP60_03075 PE=3 SV=1
MM1 pKa = 7.9SEE3 pKa = 3.94VRR5 pKa = 11.84DD6 pKa = 3.65DD7 pKa = 3.65RR8 pKa = 11.84QYY10 pKa = 11.18TEE12 pKa = 3.74THH14 pKa = 5.35EE15 pKa = 4.58WILDD19 pKa = 3.86NGDD22 pKa = 3.05GTYY25 pKa = 11.05TMGVTDD31 pKa = 4.61HH32 pKa = 6.79AQALLGDD39 pKa = 4.04MVFVEE44 pKa = 4.99LPGAGDD50 pKa = 3.4EE51 pKa = 4.64ASQEE55 pKa = 4.34DD56 pKa = 4.76EE57 pKa = 4.42FCVVEE62 pKa = 4.33SVKK65 pKa = 10.7AASGVYY71 pKa = 10.1APADD75 pKa = 3.61LEE77 pKa = 4.31VVEE80 pKa = 5.37ANEE83 pKa = 4.12EE84 pKa = 4.09LDD86 pKa = 4.53GEE88 pKa = 4.48PEE90 pKa = 4.34LVNSSCYY97 pKa = 10.39DD98 pKa = 3.68DD99 pKa = 3.55GWLVKK104 pKa = 10.08FKK106 pKa = 11.16ADD108 pKa = 4.07GIEE111 pKa = 4.02GLMSAEE117 pKa = 4.9DD118 pKa = 3.79YY119 pKa = 11.63ADD121 pKa = 3.61TLDD124 pKa = 3.41

Molecular weight: 13.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M4NK29|A0A0M4NK29_9GAMM UPF0250 protein SP60_07960 OS=Candidatus Thioglobus autotrophicus OX=1705394 GN=SP60_07960 PE=3 SV=1
MM1 pKa = 6.65KK2 pKa = 10.14QKK4 pKa = 9.65RR5 pKa = 11.84TFQPSVIKK13 pKa = 10.48RR14 pKa = 11.84KK15 pKa = 9.21RR16 pKa = 11.84KK17 pKa = 8.68HH18 pKa = 5.04GFRR21 pKa = 11.84SRR23 pKa = 11.84MSTKK27 pKa = 10.28AGRR30 pKa = 11.84AVVNARR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 8.44GRR41 pKa = 11.84KK42 pKa = 9.05RR43 pKa = 11.84LTAA46 pKa = 4.18

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1488

0

1488

436720

37

1500

293.5

32.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.808 ± 0.053

1.032 ± 0.027

5.874 ± 0.048

5.904 ± 0.052

4.284 ± 0.044

6.725 ± 0.062

2.254 ± 0.029

7.654 ± 0.057

6.708 ± 0.06

9.92 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.698 ± 0.033

4.819 ± 0.042

3.43 ± 0.033

4.303 ± 0.051

3.78 ± 0.039

6.654 ± 0.044

5.294 ± 0.037

6.735 ± 0.048

0.997 ± 0.026

3.127 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski