Kwoniella pini CBS 10737
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7827 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B9ICF3|A0A1B9ICF3_9TREE Prephenate dehydrogenase [NADP(+)] OS=Kwoniella pini CBS 10737 OX=1296096 GN=I206_00415 PE=3 SV=1
MM1 pKa = 7.27 FSKK4 pKa = 10.84 ALFLLPLLPLLSAAPLPYY22 pKa = 9.47 PLLGAGPISDD32 pKa = 4.74 LDD34 pKa = 3.94 SVINPLTQDD43 pKa = 3.75 DD44 pKa = 4.88 NLPGLPVTPGDD55 pKa = 3.96 PSPTLSNTGDD65 pKa = 3.27 GSLINGLLDD74 pKa = 3.49 TVEE77 pKa = 4.76 TITGPLNVSIGANVDD92 pKa = 3.68 LLGINTTVGLNVDD105 pKa = 4.14 LDD107 pKa = 4.19 DD108 pKa = 5.52 DD109 pKa = 4.35 EE110 pKa = 6.09 EE111 pKa = 4.62 MICGPVNGYY120 pKa = 8.61 WSNQQYY126 pKa = 10.87 NIPCACWSDD135 pKa = 3.38 SRR137 pKa = 11.84 GLVISAQLEE146 pKa = 4.01 ADD148 pKa = 3.67 LGLDD152 pKa = 3.35 QVEE155 pKa = 4.41 GLDD158 pKa = 3.79 TFLQAQIEE166 pKa = 4.45 FGGEE170 pKa = 3.62 RR171 pKa = 11.84 FTYY174 pKa = 9.36 PAYY177 pKa = 11.21 SMPTCDD183 pKa = 4.14 GDD185 pKa = 4.7 GGFNCPGGRR194 pKa = 11.84 SSNGKK199 pKa = 9.43 CSKK202 pKa = 10.37 FLAAKK207 pKa = 8.78 PRR209 pKa = 11.84 PKK211 pKa = 10.72 VLIQSTSASPASVPTANSVPTATDD235 pKa = 3.17 AVINEE240 pKa = 4.3 VPPTTLNSIPTSTIAGFDD258 pKa = 3.44 NVQPTVTPITTVSSPDD274 pKa = 2.99 QTQPVAEE281 pKa = 4.21 SQEE284 pKa = 3.91 VDD286 pKa = 4.0 DD287 pKa = 5.34 EE288 pKa = 5.12 SISADD293 pKa = 3.19 NVVLTTSTSTSTVILPATVFVEE315 pKa = 4.55 MVTTTQPTTIWATEE329 pKa = 4.23 TQTQTQTQTQTQTQTITSTSVQTQWATQTQWATTTLSNCAANDD372 pKa = 3.26 EE373 pKa = 4.67 EE374 pKa = 5.2 INVNSVSQSVQEE386 pKa = 4.24 AGYY389 pKa = 8.35 TPTPTSTYY397 pKa = 8.27 TSSSISSTISSSSSISTSSSTVSQVLPTPSINNSVPTQTSNNTGDD442 pKa = 3.98 EE443 pKa = 4.17 DD444 pKa = 5.92 DD445 pKa = 5.39 EE446 pKa = 6.05 EE447 pKa = 6.71 DD448 pKa = 4.57 DD449 pKa = 3.58 QFKK452 pKa = 10.59 PGGVIKK458 pKa = 10.73 LSHH461 pKa = 7.21 PPLKK465 pKa = 10.8 SQLKK469 pKa = 9.25 CSNGEE474 pKa = 3.91 EE475 pKa = 4.75 FKK477 pKa = 8.72 TTMCCRR483 pKa = 11.84 VDD485 pKa = 3.71 QIEE488 pKa = 4.41 LNGEE492 pKa = 4.43 CKK494 pKa = 10.0 CSKK497 pKa = 10.54 GFEE500 pKa = 4.15 NVLNLNLCLSICLGNRR516 pKa = 11.84 LPSGEE521 pKa = 4.72 CSLLDD526 pKa = 4.52 LNTNLDD532 pKa = 4.0 LGLSDD537 pKa = 4.55 ILSPLL542 pKa = 3.49
Molecular weight: 57.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.528
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.567
Grimsley 3.439
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.075
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.872
Patrickios 0.82
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|A0A1B9I632|A0A1B9I632_9TREE YbgI/family dinuclear metal center protein OS=Kwoniella pini CBS 10737 OX=1296096 GN=I206_03045 PE=3 SV=1
MM1 pKa = 7.82 PSLHH5 pKa = 6.17 QLCPDD10 pKa = 3.97 CGLPRR15 pKa = 11.84 ASPPPPLDD23 pKa = 3.27 TMFEE27 pKa = 4.18 EE28 pKa = 4.77 DD29 pKa = 4.18 FEE31 pKa = 5.16 RR32 pKa = 11.84 CFICEE37 pKa = 4.09 KK38 pKa = 10.24 ACKK41 pKa = 9.98 GLYY44 pKa = 9.87 CSSEE48 pKa = 3.78 CRR50 pKa = 11.84 LRR52 pKa = 11.84 DD53 pKa = 3.24 QGTPSPAIRR62 pKa = 11.84 ANRR65 pKa = 11.84 GPVKK69 pKa = 9.73 ITSQLPAALSPQVRR83 pKa = 11.84 ATQNIGRR90 pKa = 11.84 SPRR93 pKa = 11.84 VQPQNRR99 pKa = 11.84 GSSSISSGSSVSSSPLQSPQTNPSEE124 pKa = 4.16 ADD126 pKa = 3.22 SPKK129 pKa = 10.18 RR130 pKa = 11.84 DD131 pKa = 3.86 NFDD134 pKa = 4.39 LPPPAYY140 pKa = 7.12 PTKK143 pKa = 10.75 CFGILPASVPMKK155 pKa = 10.46 IPALTARR162 pKa = 11.84 ASPLVAPSQTPGSHH176 pKa = 6.53 GSTVYY181 pKa = 10.26 PAGASIDD188 pKa = 3.52 TLRR191 pKa = 11.84 FGRR194 pKa = 11.84 KK195 pKa = 7.94 PSAVNTVLSPNALIPRR211 pKa = 11.84 CACGKK216 pKa = 8.99 PANHH220 pKa = 7.31 RR221 pKa = 11.84 NRR223 pKa = 11.84 GSSKK227 pKa = 10.71 DD228 pKa = 3.5 RR229 pKa = 11.84 AEE231 pKa = 4.23 LADD234 pKa = 3.68 SGFSRR239 pKa = 11.84 LSLGPSVISAPHH251 pKa = 5.78 AQEE254 pKa = 4.16 EE255 pKa = 4.43 PNPRR259 pKa = 11.84 SVRR262 pKa = 11.84 IVSEE266 pKa = 4.0 SSLPGRR272 pKa = 11.84 PYY274 pKa = 10.88 QNGLGTPGRR283 pKa = 11.84 TALPLGIPSSPQVTASTSFLSRR305 pKa = 11.84 SRR307 pKa = 11.84 SDD309 pKa = 4.62 PIPSSLMAQRR319 pKa = 11.84 KK320 pKa = 8.89 AIPAAPAPAPLITNVITPSHH340 pKa = 6.85 RR341 pKa = 11.84 EE342 pKa = 3.75 LSEE345 pKa = 4.21 MPLSPIVPALGRR357 pKa = 11.84 PSRR360 pKa = 11.84 SKK362 pKa = 10.22 IALDD366 pKa = 3.32 VDD368 pKa = 4.2 VNSPRR373 pKa = 11.84 RR374 pKa = 11.84 GRR376 pKa = 11.84 SRR378 pKa = 11.84 EE379 pKa = 3.92 RR380 pKa = 11.84 QEE382 pKa = 3.92 HH383 pKa = 6.26 HH384 pKa = 6.37 VGQMTSNFGGPADD397 pKa = 4.34 RR398 pKa = 11.84 EE399 pKa = 4.26 QAPSRR404 pKa = 11.84 SRR406 pKa = 11.84 TRR408 pKa = 11.84 RR409 pKa = 11.84 EE410 pKa = 3.29 SRR412 pKa = 11.84 RR413 pKa = 11.84 RR414 pKa = 11.84 SDD416 pKa = 2.83 SRR418 pKa = 11.84 NKK420 pKa = 9.0 EE421 pKa = 3.64 RR422 pKa = 11.84 SRR424 pKa = 11.84 GGSGRR429 pKa = 11.84 PSRR432 pKa = 11.84 EE433 pKa = 3.39 RR434 pKa = 11.84 PIEE437 pKa = 4.27 EE438 pKa = 4.2 IEE440 pKa = 4.12 RR441 pKa = 11.84 TGQRR445 pKa = 11.84 SPIQQQLNSPQILPSWSRR463 pKa = 11.84 RR464 pKa = 11.84 ASEE467 pKa = 3.73 ATADD471 pKa = 3.72 RR472 pKa = 11.84 RR473 pKa = 11.84 RR474 pKa = 11.84 VLGEE478 pKa = 3.5 AAPAMRR484 pKa = 11.84 RR485 pKa = 11.84 TASGGKK491 pKa = 9.5 KK492 pKa = 10.23 SPICEE497 pKa = 3.89 RR498 pKa = 11.84 GRR500 pKa = 11.84 EE501 pKa = 3.85 RR502 pKa = 11.84 DD503 pKa = 3.28 KK504 pKa = 11.55 DD505 pKa = 3.37 PEE507 pKa = 4.03 EE508 pKa = 4.11 RR509 pKa = 11.84 KK510 pKa = 9.85 KK511 pKa = 11.3 EE512 pKa = 4.01 EE513 pKa = 4.18 LDD515 pKa = 3.47 RR516 pKa = 11.84 TSKK519 pKa = 10.6 QLSQVFGVAAVV530 pKa = 3.23
Molecular weight: 57.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.458
IPC_protein 10.35
Toseland 10.804
ProMoST 10.643
Dawson 10.862
Bjellqvist 10.628
Wikipedia 11.111
Rodwell 10.921
Grimsley 10.891
Solomon 11.067
Lehninger 11.023
Nozaki 10.818
DTASelect 10.613
Thurlkill 10.789
EMBOSS 11.228
Sillero 10.804
Patrickios 10.628
IPC_peptide 11.082
IPC2_peptide 9.926
IPC2.peptide.svr19 8.845
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7827
0
7827
4033902
49
5028
515.4
57.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.009 ± 0.027
0.957 ± 0.01
5.488 ± 0.019
6.519 ± 0.029
3.456 ± 0.016
6.708 ± 0.027
2.195 ± 0.011
5.915 ± 0.023
5.788 ± 0.029
8.868 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.964 ± 0.009
4.68 ± 0.021
6.232 ± 0.03
3.983 ± 0.02
5.378 ± 0.02
9.676 ± 0.041
5.961 ± 0.019
5.271 ± 0.021
1.323 ± 0.009
2.627 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here