Trichechus manatus latirostris papillomavirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Zurhausenvirales; Papillomaviridae; Firstpapillomavirinae; Rhopapillomavirus; Rhopapillomavirus 1

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6UYR4|H6UYR4_9PAPI Replication protein E1 OS=Trichechus manatus latirostris papillomavirus 2 OX=1144379 GN=E1 PE=3 SV=1
MM1 pKa = 7.56HH2 pKa = 7.66GEE4 pKa = 4.0TATVQDD10 pKa = 3.73VTLDD14 pKa = 4.06LSPLTIPTPDD24 pKa = 2.77VDD26 pKa = 4.34LRR28 pKa = 11.84CEE30 pKa = 3.98EE31 pKa = 4.39TVDD34 pKa = 3.97FTGVDD39 pKa = 3.3WTAVCVLQNEE49 pKa = 4.87KK50 pKa = 10.19ILQEE54 pKa = 3.96EE55 pKa = 4.05LGARR59 pKa = 11.84IGKK62 pKa = 9.66AYY64 pKa = 9.6KK65 pKa = 9.85IQAEE69 pKa = 4.32CGQCGVGVCACFLATHH85 pKa = 6.57EE86 pKa = 5.21GIVGLEE92 pKa = 3.56RR93 pKa = 11.84LLFDD97 pKa = 5.21DD98 pKa = 6.21VYY100 pKa = 10.48FLCYY104 pKa = 10.57GCGTAIKK111 pKa = 8.89YY112 pKa = 9.17HH113 pKa = 6.49HH114 pKa = 6.98GGG116 pKa = 3.31

Molecular weight: 12.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6UYR6|H6UYR6_9PAPI E4 OS=Trichechus manatus latirostris papillomavirus 2 OX=1144379 GN=E4 PE=4 SV=1
MM1 pKa = 7.1CQSGKK6 pKa = 10.27KK7 pKa = 9.53PPGVQTFGAATTMKK21 pKa = 10.15GPQRR25 pKa = 11.84LTILHH30 pKa = 6.29LWKK33 pKa = 10.17RR34 pKa = 11.84PSGMDD39 pKa = 2.79IRR41 pKa = 11.84VCGKK45 pKa = 9.72YY46 pKa = 9.95IRR48 pKa = 11.84TLRR51 pKa = 11.84LIPVPPPQTLLLATATGPPSQTARR75 pKa = 11.84HH76 pKa = 5.86RR77 pKa = 11.84RR78 pKa = 11.84PPPTGLPTDD87 pKa = 4.02PQPAEE92 pKa = 4.62GPPPTYY98 pKa = 7.65PTPQLPDD105 pKa = 5.29AIPKK109 pKa = 10.23RR110 pKa = 11.84MQQPNLGATAALPAYY125 pKa = 10.11LLGAGPFQAHH135 pKa = 6.2RR136 pKa = 11.84RR137 pKa = 11.84NPLLAQVVYY146 pKa = 10.81ALVAAEE152 pKa = 4.82LPGEE156 pKa = 4.22TASLGPFPEE165 pKa = 4.62EE166 pKa = 4.27SDD168 pKa = 3.32PGTPEE173 pKa = 4.07DD174 pKa = 4.0TLKK177 pKa = 11.25DD178 pKa = 3.7LLKK181 pKa = 10.78DD182 pKa = 3.11ILRR185 pKa = 11.84RR186 pKa = 11.84WGGDD190 pKa = 3.23LEE192 pKa = 4.37RR193 pKa = 11.84LVEE196 pKa = 4.15NVSLAVLWYY205 pKa = 10.53LRR207 pKa = 11.84TVDD210 pKa = 4.01NSPP213 pKa = 3.27

Molecular weight: 23.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2581

116

615

368.7

40.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.71 ± 0.619

2.441 ± 0.721

6.005 ± 0.413

6.122 ± 0.527

3.565 ± 0.431

7.788 ± 0.677

2.286 ± 0.185

4.339 ± 0.593

3.952 ± 0.797

8.485 ± 0.769

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.782 ± 0.388

3.138 ± 0.531

7.245 ± 1.368

4.146 ± 0.491

6.858 ± 0.297

6.005 ± 0.999

7.478 ± 0.707

5.773 ± 0.45

1.472 ± 0.277

3.41 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski