Flavobacterium micromati

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5QG01|A0A1M5QG01_9FLAO Uncharacterized protein OS=Flavobacterium micromati OX=229205 GN=SAMN05444372_11625 PE=4 SV=1
MM1 pKa = 7.59ILTSEE6 pKa = 4.67KK7 pKa = 9.97LTADD11 pKa = 3.61EE12 pKa = 4.77DD13 pKa = 3.81DD14 pKa = 3.31QRR16 pKa = 11.84IYY18 pKa = 11.01LGRR21 pKa = 11.84FAVYY25 pKa = 10.55VDD27 pKa = 3.3GVYY30 pKa = 10.18IGIYY34 pKa = 9.94DD35 pKa = 3.69VYY37 pKa = 11.51LEE39 pKa = 4.22VV40 pKa = 4.27

Molecular weight: 4.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5K2K5|A0A1M5K2K5_9FLAO 50S ribosomal protein L6 OS=Flavobacterium micromati OX=229205 GN=rplF PE=3 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGYY21 pKa = 9.53DD22 pKa = 4.32AITTDD27 pKa = 3.14KK28 pKa = 10.95PEE30 pKa = 4.34RR31 pKa = 11.84SLIAPIKK38 pKa = 10.47NSGGRR43 pKa = 11.84NSQGKK48 pKa = 6.33MTMRR52 pKa = 11.84YY53 pKa = 7.39TGGGHH58 pKa = 4.86KK59 pKa = 9.44QRR61 pKa = 11.84YY62 pKa = 8.74RR63 pKa = 11.84IIDD66 pKa = 3.78FKK68 pKa = 10.31RR69 pKa = 11.84TKK71 pKa = 10.12EE72 pKa = 4.27GIPATVKK79 pKa = 10.68SIEE82 pKa = 4.0YY83 pKa = 10.15DD84 pKa = 3.43PNRR87 pKa = 11.84TAFIALLAYY96 pKa = 10.31ADD98 pKa = 3.96GEE100 pKa = 4.24KK101 pKa = 10.14TYY103 pKa = 10.54IIAQNGLKK111 pKa = 10.25VGQKK115 pKa = 9.86LVSGPEE121 pKa = 3.92SQPEE125 pKa = 3.92IGNTLPLSKK134 pKa = 10.79VPLGTVISCIEE145 pKa = 3.9LRR147 pKa = 11.84PGQGAVIARR156 pKa = 11.84SAGTFAQLMARR167 pKa = 11.84DD168 pKa = 4.06GKK170 pKa = 10.75YY171 pKa = 9.05ATIKK175 pKa = 9.6MPSGEE180 pKa = 4.18TRR182 pKa = 11.84LILLTCSATIGAVSNSDD199 pKa = 3.15HH200 pKa = 6.2QLVVSGKK207 pKa = 9.63AGRR210 pKa = 11.84SRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPVDD229 pKa = 3.63HH230 pKa = 7.13PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 4.7PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.29GYY255 pKa = 7.29RR256 pKa = 11.84TRR258 pKa = 11.84SKK260 pKa = 10.85KK261 pKa = 10.4NPSNKK266 pKa = 10.11YY267 pKa = 8.41IVEE270 pKa = 4.07RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.24KK274 pKa = 10.1

Molecular weight: 29.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3204

0

3204

1040682

29

4779

324.8

36.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.529 ± 0.048

0.753 ± 0.014

5.258 ± 0.031

6.298 ± 0.048

5.471 ± 0.038

6.107 ± 0.041

1.6 ± 0.018

8.644 ± 0.039

8.064 ± 0.062

9.298 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.023

6.454 ± 0.041

3.256 ± 0.028

3.472 ± 0.025

3.216 ± 0.031

6.534 ± 0.033

5.839 ± 0.069

6.144 ± 0.039

0.956 ± 0.017

3.885 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski