Candidatus Enterovibrio escacola
Average proteome isoelectric point is 7.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3193 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2A5T0S9|A0A2A5T0S9_9GAMM Mobile element protein OS=Candidatus Enterovibrio escacola OX=1927127 GN=BTN49_2742 PE=4 SV=1
MM1 pKa = 8.19 DD2 pKa = 5.91 DD3 pKa = 4.12 KK4 pKa = 11.54 LYY6 pKa = 10.64 LPVVIGVDD14 pKa = 3.07 NTSYY18 pKa = 11.29 KK19 pKa = 10.46 EE20 pKa = 3.82 VLAVVDD26 pKa = 5.15 GYY28 pKa = 11.23 RR29 pKa = 11.84 EE30 pKa = 4.36 SEE32 pKa = 4.4 VSWLEE37 pKa = 3.74 VLSQLTCQDD46 pKa = 3.33 INISPP51 pKa = 3.88
Molecular weight: 5.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.961
IPC_protein 3.808
Toseland 3.617
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.541
Solomon 3.77
Lehninger 3.732
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.935
Patrickios 1.901
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.838
Protein with the highest isoelectric point:
>tr|A0A2A5T542|A0A2A5T542_9GAMM RNA polymerase sigma factor RpoH OS=Candidatus Enterovibrio escacola OX=1927127 GN=rpoH PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 9.99 GGRR28 pKa = 11.84 RR29 pKa = 11.84 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.14 GRR39 pKa = 11.84 KK40 pKa = 8.23 QLSKK44 pKa = 11.23
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3193
0
3193
536259
37
1605
167.9
18.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.643 ± 0.052
1.328 ± 0.019
5.162 ± 0.038
5.76 ± 0.049
4.203 ± 0.036
6.372 ± 0.054
2.455 ± 0.025
7.498 ± 0.043
6.305 ± 0.048
10.303 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.07 ± 0.025
4.633 ± 0.033
3.531 ± 0.029
3.779 ± 0.034
5.254 ± 0.039
6.582 ± 0.044
5.499 ± 0.033
7.221 ± 0.043
1.159 ± 0.02
3.243 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here