Oryza meridionalis
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 40192 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0E0DXQ5|A0A0E0DXQ5_9ORYZ Uncharacterized protein OS=Oryza meridionalis OX=40149 PE=4 SV=1
MM1 pKa = 7.53 GKK3 pKa = 9.36 NASRR7 pKa = 11.84 LAAAAAVVLFALLLSLRR24 pKa = 11.84 QLEE27 pKa = 4.36 AADD30 pKa = 4.63 DD31 pKa = 4.16 VSVSCSDD38 pKa = 3.86 VIADD42 pKa = 3.6 VTPCLGFLQGDD53 pKa = 3.85 DD54 pKa = 3.91 DD55 pKa = 4.71 HH56 pKa = 7.39 PSGEE60 pKa = 4.49 CCDD63 pKa = 3.86 GLSGLVAAAATTEE76 pKa = 3.94 DD77 pKa = 3.18 RR78 pKa = 11.84 QAACEE83 pKa = 4.01 CLKK86 pKa = 10.94 SAVSGQFTAVEE97 pKa = 3.9 AAPARR102 pKa = 11.84 DD103 pKa = 3.64 LPADD107 pKa = 4.22 CGLSLPYY114 pKa = 10.13 TFSPDD119 pKa = 3.35 DD120 pKa = 3.44
Molecular weight: 12.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.846
IPC_protein 3.821
Toseland 3.592
ProMoST 3.935
Dawson 3.821
Bjellqvist 4.037
Wikipedia 3.795
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.215
Thurlkill 3.668
EMBOSS 3.795
Sillero 3.948
Patrickios 0.896
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.863
Protein with the highest isoelectric point:
>tr|A0A0E0C4J5|A0A0E0C4J5_9ORYZ N-acetyltransferase domain-containing protein OS=Oryza meridionalis OX=40149 PE=4 SV=1
MM1 pKa = 7.36 VLRR4 pKa = 11.84 TVRR7 pKa = 11.84 KK8 pKa = 9.71 SSIKK12 pKa = 10.4 GSPATPKK19 pKa = 9.18 TRR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 SPTLLPLRR31 pKa = 11.84 LLMVLIRR38 pKa = 11.84 ANLRR42 pKa = 11.84 FQQVSSFASFARR54 pKa = 11.84 QKK56 pKa = 10.45 VMHH59 pKa = 7.24 RR60 pKa = 11.84 GTMKK64 pKa = 10.92 AFGLGLLRR72 pKa = 11.84 KK73 pKa = 9.36
Molecular weight: 8.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
29137
11055
40192
16856824
7
5440
419.4
46.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.174 ± 0.019
1.898 ± 0.006
5.389 ± 0.009
6.025 ± 0.014
3.631 ± 0.007
7.42 ± 0.014
2.49 ± 0.006
4.461 ± 0.01
4.882 ± 0.012
9.456 ± 0.016
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.423 ± 0.004
3.563 ± 0.01
5.588 ± 0.014
3.437 ± 0.01
6.388 ± 0.013
8.428 ± 0.014
4.799 ± 0.006
6.682 ± 0.01
1.314 ± 0.004
2.545 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here