Lautropia mirabilis ATCC 51599
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2665 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7RU53|E7RU53_9BURK Serine hydroxymethyltransferase OS=Lautropia mirabilis ATCC 51599 OX=887898 GN=glyA PE=3 SV=1
MM1 pKa = 7.65 SEE3 pKa = 4.12 VSEE6 pKa = 4.47 MPPPLLFTDD15 pKa = 4.17 SAATKK20 pKa = 9.86 VGEE23 pKa = 4.65 LIMEE27 pKa = 4.98 EE28 pKa = 4.36 GNPDD32 pKa = 2.93 LKK34 pKa = 11.13 LRR36 pKa = 11.84 VFVQGGGCSGFQYY49 pKa = 10.98 GFTFDD54 pKa = 3.92 EE55 pKa = 5.44 DD56 pKa = 3.75 VAEE59 pKa = 5.41 DD60 pKa = 3.72 DD61 pKa = 4.4 TVMEE65 pKa = 4.57 KK66 pKa = 10.93 NGVTLLIDD74 pKa = 3.64 AMSYY78 pKa = 10.32 QYY80 pKa = 11.5 LVGAEE85 pKa = 4.01 IDD87 pKa = 4.05 YY88 pKa = 11.2 KK89 pKa = 11.38 DD90 pKa = 4.78 DD91 pKa = 3.43 LHH93 pKa = 7.53 GSQFVIKK100 pKa = 10.02 NPNAATTCGCGSSFSAA116 pKa = 4.79
Molecular weight: 12.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.935
IPC_protein 3.884
Toseland 3.681
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.795
Nozaki 3.986
DTASelect 4.177
Thurlkill 3.732
EMBOSS 3.795
Sillero 3.999
Patrickios 1.901
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.888
Protein with the highest isoelectric point:
>tr|E7RZP2|E7RZP2_9BURK Uncharacterized protein OS=Lautropia mirabilis ATCC 51599 OX=887898 GN=HMPREF0551_2156 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.17 QPSVCRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.78 RR14 pKa = 11.84 THH16 pKa = 6.44 GFLVRR21 pKa = 11.84 QKK23 pKa = 10.8 SRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.72 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.891
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.164
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.901
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.096
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2665
0
2665
918189
39
2788
344.5
37.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.041 ± 0.064
0.854 ± 0.014
5.527 ± 0.038
5.319 ± 0.051
3.246 ± 0.03
8.36 ± 0.055
2.353 ± 0.024
4.444 ± 0.039
3.052 ± 0.035
10.582 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.493 ± 0.022
2.715 ± 0.028
5.649 ± 0.037
4.379 ± 0.036
7.256 ± 0.05
5.631 ± 0.036
5.228 ± 0.045
7.253 ± 0.042
1.445 ± 0.02
2.173 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here