Roseovarius mucosus DSM 17069

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius; Roseovarius mucosus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4182 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0HPA4|A0A0A0HPA4_9RHOB Uncharacterized protein OS=Roseovarius mucosus DSM 17069 OX=1288298 GN=rosmuc_02597 PE=4 SV=1
MM1 pKa = 7.5SFKK4 pKa = 8.72TTFALTTVAVSLGAAAQGDD23 pKa = 3.94DD24 pKa = 3.26HH25 pKa = 6.13VTLRR29 pKa = 11.84GYY31 pKa = 11.21ALAADD36 pKa = 4.74GGTLVTMADD45 pKa = 4.02LSQPGAVEE53 pKa = 4.72TYY55 pKa = 11.37ALATPLRR62 pKa = 11.84AIAYY66 pKa = 9.19RR67 pKa = 11.84PVTGQLLGYY76 pKa = 9.94SDD78 pKa = 4.12GAIFAVDD85 pKa = 3.87PEE87 pKa = 4.61SGQLTDD93 pKa = 4.66LGATFMEE100 pKa = 4.9DD101 pKa = 3.09AVIGEE106 pKa = 4.39GAVAFDD112 pKa = 4.7FNNQIDD118 pKa = 3.77AVRR121 pKa = 11.84AVGADD126 pKa = 3.4GSNLVYY132 pKa = 10.26FPQGFGDD139 pKa = 3.35NDD141 pKa = 3.03EE142 pKa = 4.67RR143 pKa = 11.84ANSVRR148 pKa = 11.84RR149 pKa = 11.84FTDD152 pKa = 2.93AFYY155 pKa = 11.22VAGDD159 pKa = 3.69MSAGSDD165 pKa = 3.24PVIFANAYY173 pKa = 7.4TNAIPGAKK181 pKa = 9.59AASTAQYY188 pKa = 11.3ALDD191 pKa = 4.28AGADD195 pKa = 3.72ALVTLANNAGEE206 pKa = 4.13LASVGHH212 pKa = 6.92LSLDD216 pKa = 3.49GATVDD221 pKa = 3.5VSDD224 pKa = 3.69WGGFDD229 pKa = 2.72IVSPEE234 pKa = 4.13EE235 pKa = 4.29GTDD238 pKa = 3.09MAYY241 pKa = 10.21AILQIEE247 pKa = 4.49GAEE250 pKa = 4.21TAGLYY255 pKa = 10.62SVDD258 pKa = 4.32LGSGALTEE266 pKa = 4.17MADD269 pKa = 3.35LGMGGFTGFAVAQAQQ284 pKa = 3.27

Molecular weight: 28.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0HND4|A0A0A0HND4_9RHOB Iron-regulated ABC transporter permease protein SufD OS=Roseovarius mucosus DSM 17069 OX=1288298 GN=rosmuc_01557 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.83HH14 pKa = 4.64RR15 pKa = 11.84HH16 pKa = 3.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.57SLCAA44 pKa = 3.82

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4182

0

4182

1286318

20

5490

307.6

33.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.587 ± 0.05

0.918 ± 0.013

5.932 ± 0.04

5.823 ± 0.033

3.621 ± 0.023

8.789 ± 0.047

2.115 ± 0.02

5.111 ± 0.027

2.853 ± 0.036

10.257 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.791 ± 0.022

2.527 ± 0.023

5.111 ± 0.028

3.207 ± 0.02

7.013 ± 0.048

4.967 ± 0.026

5.533 ± 0.051

7.256 ± 0.033

1.395 ± 0.017

2.193 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski