Gordonia paraffinivorans NBRC 108238

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Gordoniaceae; Gordonia; Gordonia paraffinivorans

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4191 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M3VHV4|M3VHV4_9ACTN Putative acyl-CoA carboxylase beta chain OS=Gordonia paraffinivorans NBRC 108238 OX=1223543 GN=GP2_003_01070 PE=3 SV=1
MM1 pKa = 7.64GSPYY5 pKa = 10.79DD6 pKa = 4.01PNQPTQMGPQGGQQPGWPGPQPGAGGPGFGAQPGQPYY43 pKa = 9.87AQQPYY48 pKa = 7.81GQQYY52 pKa = 9.3PPQPGQQPYY61 pKa = 8.91GQQPYY66 pKa = 9.08GQQPYY71 pKa = 9.77GPPPQPPSSSGGGKK85 pKa = 9.26KK86 pKa = 7.25WWFIGGGGVLLILIVVVAVVLAFTLGGGDD115 pKa = 5.09DD116 pKa = 4.28EE117 pKa = 6.19PDD119 pKa = 3.43VPTASAVDD127 pKa = 3.53MLLPEE132 pKa = 4.81SEE134 pKa = 4.65FPDD137 pKa = 3.07ITGEE141 pKa = 4.17FEE143 pKa = 5.59LDD145 pKa = 3.27TAQSGDD151 pKa = 4.01DD152 pKa = 4.33DD153 pKa = 5.95DD154 pKa = 6.5ISVDD158 pKa = 3.45NEE160 pKa = 3.54KK161 pKa = 10.55CARR164 pKa = 11.84LVDD167 pKa = 4.1SQSGGDD173 pKa = 3.53YY174 pKa = 10.59AQRR177 pKa = 11.84EE178 pKa = 4.47LTEE181 pKa = 4.4TPSSGEE187 pKa = 3.67IFFGLDD193 pKa = 2.78AYY195 pKa = 10.7SAEE198 pKa = 4.31VTKK201 pKa = 10.24PADD204 pKa = 3.45DD205 pKa = 4.2TYY207 pKa = 11.6DD208 pKa = 3.35TFDD211 pKa = 4.76DD212 pKa = 3.93VLAACSSFTLTLKK225 pKa = 10.79DD226 pKa = 3.92DD227 pKa = 4.33GGDD230 pKa = 3.08IPVAVKK236 pKa = 10.61LEE238 pKa = 4.14KK239 pKa = 10.36ADD241 pKa = 4.46LPIDD245 pKa = 3.9SNYY248 pKa = 10.57KK249 pKa = 8.59AFNMFGEE256 pKa = 4.3FSIDD260 pKa = 3.28AAGDD264 pKa = 3.62EE265 pKa = 4.55VEE267 pKa = 4.66VYY269 pKa = 10.7QIGTYY274 pKa = 10.45VYY276 pKa = 9.42GEE278 pKa = 3.94EE279 pKa = 4.11RR280 pKa = 11.84GVSFGVGYY288 pKa = 11.0NSFSDD293 pKa = 3.51EE294 pKa = 4.15KK295 pKa = 11.17LSVDD299 pKa = 3.88SQIEE303 pKa = 4.24NNLSQMFAKK312 pKa = 9.83QHH314 pKa = 4.82QRR316 pKa = 11.84IKK318 pKa = 10.81DD319 pKa = 3.44ASS321 pKa = 3.42

Molecular weight: 34.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M3VEE9|M3VEE9_9ACTN Putative LuxR family transcriptional regulator OS=Gordonia paraffinivorans NBRC 108238 OX=1223543 GN=GP2_017_00970 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84KK18 pKa = 8.65HH19 pKa = 4.47GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.79GRR42 pKa = 11.84AKK44 pKa = 9.67LTAA47 pKa = 4.21

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4191

0

4191

1391700

41

6116

332.1

35.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.672 ± 0.046

0.734 ± 0.01

6.607 ± 0.032

5.794 ± 0.035

2.977 ± 0.019

8.937 ± 0.035

2.136 ± 0.016

4.421 ± 0.023

2.301 ± 0.031

9.464 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.965 ± 0.016

2.071 ± 0.018

5.826 ± 0.033

2.594 ± 0.019

7.518 ± 0.04

5.546 ± 0.025

6.124 ± 0.022

8.879 ± 0.034

1.408 ± 0.017

2.027 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski