Pragia fontium

Taxonomy: cellular organisms; Bacteria; Proteobacteria;

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3326 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G3CP09|A0A0G3CP09_9GAMM Arginine exporter protein OS=Pragia fontium OX=82985 GN=QQ39_03165 PE=3 SV=1
MM1 pKa = 7.49HH2 pKa = 7.66LLSDD6 pKa = 4.17EE7 pKa = 4.64EE8 pKa = 4.64IEE10 pKa = 5.49ALDD13 pKa = 3.87VDD15 pKa = 4.45NLPEE19 pKa = 4.69LGSIEE24 pKa = 4.79FNDD27 pKa = 4.13PNDD30 pKa = 3.6MPAEE34 pKa = 3.89MRR36 pKa = 11.84EE37 pKa = 3.88QLFLSMVEE45 pKa = 4.04QTDD48 pKa = 3.58DD49 pKa = 3.63PTYY52 pKa = 10.41MLALADD58 pKa = 4.83LYY60 pKa = 11.27LLEE63 pKa = 5.42LDD65 pKa = 4.36RR66 pKa = 11.84PQDD69 pKa = 3.34AKK71 pKa = 11.25LWLDD75 pKa = 3.23KK76 pKa = 10.63MLALDD81 pKa = 4.21YY82 pKa = 10.8LPAYY86 pKa = 7.92ITQAQMYY93 pKa = 8.45MYY95 pKa = 10.62GKK97 pKa = 10.59YY98 pKa = 10.08FDD100 pKa = 4.08QDD102 pKa = 3.15LDD104 pKa = 3.5KK105 pKa = 11.09AAQLFEE111 pKa = 4.06QLIEE115 pKa = 5.67RR116 pKa = 11.84YY117 pKa = 7.45TQDD120 pKa = 4.62DD121 pKa = 4.1NIEE124 pKa = 4.0QHH126 pKa = 5.98SEE128 pKa = 3.74QLAFCYY134 pKa = 10.4LSLAQLYY141 pKa = 8.28QAQEE145 pKa = 5.29DD146 pKa = 4.33IPQMLSQYY154 pKa = 10.61FNALQLSSQEE164 pKa = 4.13AASTMAHH171 pKa = 6.12MFDD174 pKa = 5.04PDD176 pKa = 3.48ITDD179 pKa = 4.68DD180 pKa = 4.11PACKK184 pKa = 10.32AEE186 pKa = 5.26LSLLHH191 pKa = 6.65CALLTLSNEE200 pKa = 4.14FLLQQSEE207 pKa = 4.2QTEE210 pKa = 4.14DD211 pKa = 3.72SEE213 pKa = 5.46LKK215 pKa = 10.41QLLLSHH221 pKa = 6.19YY222 pKa = 10.77QMAAHH227 pKa = 6.78EE228 pKa = 4.96LEE230 pKa = 4.17QSDD233 pKa = 4.11NAGQLTSAQQEE244 pKa = 4.58KK245 pKa = 10.53INEE248 pKa = 4.54LIEE251 pKa = 3.86AWNRR255 pKa = 11.84GEE257 pKa = 4.14PQVLMDD263 pKa = 6.13AIVSYY268 pKa = 11.34INGG271 pKa = 3.57

Molecular weight: 31.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G3CFV6|A0A0G3CFV6_9GAMM Cyclic nucleotide-binding protein OS=Pragia fontium OX=82985 GN=QQ39_01160 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.36GRR39 pKa = 11.84TRR41 pKa = 11.84LTVSSS46 pKa = 3.96

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3326

0

3326

1068109

29

4546

321.1

35.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.716 ± 0.045

1.08 ± 0.018

5.264 ± 0.037

5.591 ± 0.05

3.833 ± 0.033

7.406 ± 0.067

2.141 ± 0.021

6.654 ± 0.041

4.739 ± 0.041

10.661 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.671 ± 0.027

4.307 ± 0.05

4.184 ± 0.03

4.713 ± 0.048

5.009 ± 0.05

6.511 ± 0.046

5.361 ± 0.047

6.776 ± 0.038

1.315 ± 0.017

3.067 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski