Amino acid dipepetide frequency for Pragia fontium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.111AlaAla: 8.111 ± 0.12	1.015AlaCys: 1.015 ± 0.036	4.638AlaAsp: 4.638 ± 0.065	5.742AlaGlu: 5.742 ± 0.102	3.191AlaPhe: 3.191 ± 0.058	6.738AlaGly: 6.738 ± 0.116	1.527AlaHis: 1.527 ± 0.037	6.173AlaIle: 6.173 ± 0.081	4.35AlaLys: 4.35 ± 0.081	10.051AlaLeu: 10.051 ± 0.116	2.518AlaMet: 2.518 ± 0.058	3.469AlaAsn: 3.469 ± 0.074	2.98AlaPro: 2.98 ± 0.056	3.826AlaGln: 3.826 ± 0.079	4.045AlaArg: 4.045 ± 0.072	5.218AlaSer: 5.218 ± 0.083	4.442AlaThr: 4.442 ± 0.074	5.743AlaVal: 5.743 ± 0.087	1.029AlaTrp: 1.029 ± 0.032	2.357AlaTyr: 2.357 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.877CysAla: 0.877 ± 0.036	0.173CysCys: 0.173 ± 0.015	0.567CysAsp: 0.567 ± 0.025	0.565CysGlu: 0.565 ± 0.024	0.437CysPhe: 0.437 ± 0.021	1.038CysGly: 1.038 ± 0.037	0.389CysHis: 0.389 ± 0.025	0.683CysIle: 0.683 ± 0.026	0.381CysLys: 0.381 ± 0.019	1.035CysLeu: 1.035 ± 0.036	0.229CysMet: 0.229 ± 0.017	0.383CysAsn: 0.383 ± 0.019	0.499CysPro: 0.499 ± 0.025	0.521CysGln: 0.521 ± 0.025	0.58CysArg: 0.58 ± 0.024	0.721CysSer: 0.721 ± 0.025	0.494CysThr: 0.494 ± 0.022	0.688CysVal: 0.688 ± 0.026	0.178CysTrp: 0.178 ± 0.013	0.358CysTyr: 0.358 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	4.422AspAla: 4.422 ± 0.079	0.574AspCys: 0.574 ± 0.023	2.841AspAsp: 2.841 ± 0.06	3.406AspGlu: 3.406 ± 0.07	2.156AspPhe: 2.156 ± 0.052	4.002AspGly: 4.002 ± 0.101	0.915AspHis: 0.915 ± 0.027	4.024AspIle: 4.024 ± 0.066	3.041AspLys: 3.041 ± 0.055	4.86AspLeu: 4.86 ± 0.077	1.452AspMet: 1.452 ± 0.038	2.558AspAsn: 2.558 ± 0.055	2.034AspPro: 2.034 ± 0.049	1.613AspGln: 1.613 ± 0.04	2.654AspArg: 2.654 ± 0.051	3.225AspSer: 3.225 ± 0.067	2.448AspThr: 2.448 ± 0.059	3.633AspVal: 3.633 ± 0.06	0.786AspTrp: 0.786 ± 0.033	1.999AspTyr: 1.999 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	4.664GluAla: 4.664 ± 0.08	0.506GluCys: 0.506 ± 0.024	2.393GluAsp: 2.393 ± 0.058	3.118GluGlu: 3.118 ± 0.081	1.962GluPhe: 1.962 ± 0.05	3.384GluGly: 3.384 ± 0.062	1.32GluHis: 1.32 ± 0.036	3.518GluIle: 3.518 ± 0.065	3.337GluLys: 3.337 ± 0.073	6.248GluLeu: 6.248 ± 0.101	1.711GluMet: 1.711 ± 0.045	2.434GluAsn: 2.434 ± 0.053	1.986GluPro: 1.986 ± 0.05	3.842GluGln: 3.842 ± 0.091	3.505GluArg: 3.505 ± 0.065	3.252GluSer: 3.252 ± 0.06	2.727GluThr: 2.727 ± 0.061	3.706GluVal: 3.706 ± 0.072	0.733GluTrp: 0.733 ± 0.032	1.559GluTyr: 1.559 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	3.111PheAla: 3.111 ± 0.059	0.53PheCys: 0.53 ± 0.023	2.378PheAsp: 2.378 ± 0.046	1.769PheGlu: 1.769 ± 0.042	1.633PhePhe: 1.633 ± 0.052	3.142PheGly: 3.142 ± 0.067	0.728PheHis: 0.728 ± 0.027	2.892PheIle: 2.892 ± 0.06	1.548PheLys: 1.548 ± 0.043	3.283PheLeu: 3.283 ± 0.064	0.968PheMet: 0.968 ± 0.031	2.011PheAsn: 2.011 ± 0.047	1.444PhePro: 1.444 ± 0.037	1.199PheGln: 1.199 ± 0.036	1.669PheArg: 1.669 ± 0.043	3.386PheSer: 3.386 ± 0.073	2.296PheThr: 2.296 ± 0.046	2.388PheVal: 2.388 ± 0.05	0.566PheTrp: 0.566 ± 0.026	1.39PheTyr: 1.39 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	5.936GlyAla: 5.936 ± 0.097	1.005GlyCys: 1.005 ± 0.035	3.785GlyAsp: 3.785 ± 0.074	4.198GlyGlu: 4.198 ± 0.068	3.196GlyPhe: 3.196 ± 0.059	5.507GlyGly: 5.507 ± 0.115	1.641GlyHis: 1.641 ± 0.041	5.507GlyIle: 5.507 ± 0.092	4.152GlyLys: 4.152 ± 0.066	7.528GlyLeu: 7.528 ± 0.096	2.225GlyMet: 2.225 ± 0.052	3.116GlyAsn: 3.116 ± 0.098	1.843GlyPro: 1.843 ± 0.047	3.001GlyGln: 3.001 ± 0.063	3.369GlyArg: 3.369 ± 0.062	4.671GlySer: 4.671 ± 0.098	3.949GlyThr: 3.949 ± 0.105	5.405GlyVal: 5.405 ± 0.078	1.198GlyTrp: 1.198 ± 0.033	2.833GlyTyr: 2.833 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	1.583HisAla: 1.583 ± 0.035	0.32HisCys: 0.32 ± 0.02	1.108HisAsp: 1.108 ± 0.036	0.962HisGlu: 0.962 ± 0.033	0.96HisPhe: 0.96 ± 0.034	1.586HisGly: 1.586 ± 0.042	0.684HisHis: 0.684 ± 0.027	1.427HisIle: 1.427 ± 0.037	0.838HisLys: 0.838 ± 0.027	2.096HisLeu: 2.096 ± 0.049	0.491HisMet: 0.491 ± 0.021	0.904HisAsn: 0.904 ± 0.029	1.223HisPro: 1.223 ± 0.045	1.334HisGln: 1.334 ± 0.036	1.165HisArg: 1.165 ± 0.033	1.355HisSer: 1.355 ± 0.038	1.04HisThr: 1.04 ± 0.03	1.124HisVal: 1.124 ± 0.035	0.371HisTrp: 0.371 ± 0.019	0.841HisTyr: 0.841 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.624IleAla: 6.624 ± 0.088	0.761IleCys: 0.761 ± 0.029	4.066IleAsp: 4.066 ± 0.063	3.944IleGlu: 3.944 ± 0.073	2.357IlePhe: 2.357 ± 0.054	5.337IleGly: 5.337 ± 0.08	1.363IleHis: 1.363 ± 0.036	4.199IleIle: 4.199 ± 0.078	3.134IleLys: 3.134 ± 0.059	5.81IleLeu: 5.81 ± 0.081	1.434IleMet: 1.434 ± 0.045	3.279IleAsn: 3.279 ± 0.057	3.016IlePro: 3.016 ± 0.054	2.409IleGln: 2.409 ± 0.053	3.032IleArg: 3.032 ± 0.047	4.815IleSer: 4.815 ± 0.077	4.058IleThr: 4.058 ± 0.07	4.314IleVal: 4.314 ± 0.073	0.767IleTrp: 0.767 ± 0.031	1.823IleTyr: 1.823 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	4.206LysAla: 4.206 ± 0.079	0.286LysCys: 0.286 ± 0.018	2.445LysAsp: 2.445 ± 0.048	2.713LysGlu: 2.713 ± 0.059	1.333LysPhe: 1.333 ± 0.037	3.161LysGly: 3.161 ± 0.068	0.929LysHis: 0.929 ± 0.031	2.937LysIle: 2.937 ± 0.061	2.526LysLys: 2.526 ± 0.064	4.99LysLeu: 4.99 ± 0.073	1.431LysMet: 1.431 ± 0.042	2.195LysAsn: 2.195 ± 0.051	2.286LysPro: 2.286 ± 0.049	2.642LysGln: 2.642 ± 0.07	2.596LysArg: 2.596 ± 0.049	2.743LysSer: 2.743 ± 0.052	2.793LysThr: 2.793 ± 0.058	3.439LysVal: 3.439 ± 0.065	0.457LysTrp: 0.457 ± 0.022	1.285LysTyr: 1.285 ± 0.041	0.0LysXaa: 0.0 ± 0.0
Leu	10.132LeuAla: 10.132 ± 0.125	1.168LeuCys: 1.168 ± 0.038	5.447LeuAsp: 5.447 ± 0.077	5.445LeuGlu: 5.445 ± 0.08	4.249LeuPhe: 4.249 ± 0.081	7.239LeuGly: 7.239 ± 0.091	2.002LeuHis: 2.002 ± 0.047	6.724LeuIle: 6.724 ± 0.095	5.015LeuLys: 5.015 ± 0.075	11.401LeuLeu: 11.401 ± 0.156	2.877LeuMet: 2.877 ± 0.065	4.808LeuAsn: 4.808 ± 0.08	5.421LeuPro: 5.421 ± 0.089	4.048LeuGln: 4.048 ± 0.073	5.175LeuArg: 5.175 ± 0.084	8.148LeuSer: 8.148 ± 0.11	6.512LeuThr: 6.512 ± 0.079	6.84LeuVal: 6.84 ± 0.092	1.196LeuTrp: 1.196 ± 0.039	2.762LeuTyr: 2.762 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	2.634MetAla: 2.634 ± 0.06	0.194MetCys: 0.194 ± 0.013	1.238MetAsp: 1.238 ± 0.032	1.204MetGlu: 1.204 ± 0.035	0.9MetPhe: 0.9 ± 0.03	1.857MetGly: 1.857 ± 0.045	0.473MetHis: 0.473 ± 0.021	1.599MetIle: 1.599 ± 0.04	1.473MetLys: 1.473 ± 0.032	3.041MetLeu: 3.041 ± 0.06	0.837MetMet: 0.837 ± 0.029	1.231MetAsn: 1.231 ± 0.032	1.293MetPro: 1.293 ± 0.035	1.106MetGln: 1.106 ± 0.039	1.284MetArg: 1.284 ± 0.034	1.977MetSer: 1.977 ± 0.047	1.641MetThr: 1.641 ± 0.045	1.964MetVal: 1.964 ± 0.046	0.23MetTrp: 0.23 ± 0.017	0.537MetTyr: 0.537 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	3.465AsnAla: 3.465 ± 0.066	0.39AsnCys: 0.39 ± 0.021	2.309AsnAsp: 2.309 ± 0.052	2.075AsnGlu: 2.075 ± 0.049	1.401AsnPhe: 1.401 ± 0.034	3.59AsnGly: 3.59 ± 0.101	0.995AsnHis: 0.995 ± 0.029	3.12AsnIle: 3.12 ± 0.07	2.112AsnLys: 2.112 ± 0.049	3.976AsnLeu: 3.976 ± 0.07	1.081AsnMet: 1.081 ± 0.03	2.184AsnAsn: 2.184 ± 0.066	2.217AsnPro: 2.217 ± 0.044	2.251AsnGln: 2.251 ± 0.056	2.063AsnArg: 2.063 ± 0.043	2.716AsnSer: 2.716 ± 0.072	2.364AsnThr: 2.364 ± 0.074	2.773AsnVal: 2.773 ± 0.07	0.615AsnTrp: 0.615 ± 0.026	1.374AsnTyr: 1.374 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	3.693ProAla: 3.693 ± 0.062	0.381ProCys: 0.381 ± 0.017	2.574ProAsp: 2.574 ± 0.051	3.324ProGlu: 3.324 ± 0.061	1.747ProPhe: 1.747 ± 0.039	2.72ProGly: 2.72 ± 0.052	0.92ProHis: 0.92 ± 0.033	2.5ProIle: 2.5 ± 0.054	1.769ProLys: 1.769 ± 0.046	4.685ProLeu: 4.685 ± 0.071	1.077ProMet: 1.077 ± 0.037	1.527ProAsn: 1.527 ± 0.044	1.441ProPro: 1.441 ± 0.04	2.045ProGln: 2.045 ± 0.047	1.583ProArg: 1.583 ± 0.042	2.376ProSer: 2.376 ± 0.055	2.276ProThr: 2.276 ± 0.045	3.285ProVal: 3.285 ± 0.053	0.542ProTrp: 0.542 ± 0.024	1.377ProTyr: 1.377 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	4.432GlnAla: 4.432 ± 0.079	0.403GlnCys: 0.403 ± 0.019	2.083GlnAsp: 2.083 ± 0.049	2.244GlnGlu: 2.244 ± 0.053	1.631GlnPhe: 1.631 ± 0.041	3.205GlnGly: 3.205 ± 0.057	1.224GlnHis: 1.224 ± 0.044	2.792GlnIle: 2.792 ± 0.057	1.982GlnLys: 1.982 ± 0.047	5.416GlnLeu: 5.416 ± 0.116	1.157GlnMet: 1.157 ± 0.032	1.711GlnAsn: 1.711 ± 0.045	2.07GlnPro: 2.07 ± 0.046	3.805GlnGln: 3.805 ± 0.117	2.927GlnArg: 2.927 ± 0.066	2.733GlnSer: 2.733 ± 0.056	2.255GlnThr: 2.255 ± 0.06	3.083GlnVal: 3.083 ± 0.048	0.667GlnTrp: 0.667 ± 0.029	1.314GlnTyr: 1.314 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	3.69ArgAla: 3.69 ± 0.069	0.54ArgCys: 0.54 ± 0.022	2.604ArgAsp: 2.604 ± 0.053	3.117ArgGlu: 3.117 ± 0.071	2.313ArgPhe: 2.313 ± 0.049	3.068ArgGly: 3.068 ± 0.056	1.348ArgHis: 1.348 ± 0.039	3.446ArgIle: 3.446 ± 0.062	2.289ArgLys: 2.289 ± 0.057	5.802ArgLeu: 5.802 ± 0.091	1.31ArgMet: 1.31 ± 0.035	1.955ArgAsn: 1.955 ± 0.045	1.902ArgPro: 1.902 ± 0.047	2.843ArgGln: 2.843 ± 0.065	2.782ArgArg: 2.782 ± 0.066	2.744ArgSer: 2.744 ± 0.053	2.228ArgThr: 2.228 ± 0.041	3.241ArgVal: 3.241 ± 0.062	0.786ArgTrp: 0.786 ± 0.029	2.084ArgTyr: 2.084 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	5.675SerAla: 5.675 ± 0.081	0.652SerCys: 0.652 ± 0.027	3.456SerAsp: 3.456 ± 0.062	3.5SerGlu: 3.5 ± 0.064	2.543SerPhe: 2.543 ± 0.05	6.006SerGly: 6.006 ± 0.123	1.514SerHis: 1.514 ± 0.039	4.01SerIle: 4.01 ± 0.071	2.57SerLys: 2.57 ± 0.052	7.259SerLeu: 7.259 ± 0.091	1.668SerMet: 1.668 ± 0.043	2.496SerAsn: 2.496 ± 0.067	2.598SerPro: 2.598 ± 0.055	3.034SerGln: 3.034 ± 0.057	3.222SerArg: 3.222 ± 0.054	4.144SerSer: 4.144 ± 0.072	3.246SerThr: 3.246 ± 0.067	4.574SerVal: 4.574 ± 0.085	0.923SerTrp: 0.923 ± 0.031	2.019SerTyr: 2.019 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	4.682ThrAla: 4.682 ± 0.076	0.532ThrCys: 0.532 ± 0.025	2.825ThrAsp: 2.825 ± 0.056	2.968ThrGlu: 2.968 ± 0.054	1.954ThrPhe: 1.954 ± 0.042	4.456ThrGly: 4.456 ± 0.1	1.141ThrHis: 1.141 ± 0.038	3.487ThrIle: 3.487 ± 0.064	1.989ThrLys: 1.989 ± 0.047	6.781ThrLeu: 6.781 ± 0.105	1.129ThrMet: 1.129 ± 0.032	1.946ThrAsn: 1.946 ± 0.057	2.887ThrPro: 2.887 ± 0.058	2.28ThrGln: 2.28 ± 0.05	2.431ThrArg: 2.431 ± 0.045	3.31ThrSer: 3.31 ± 0.066	2.848ThrThr: 2.848 ± 0.076	3.914ThrVal: 3.914 ± 0.096	0.598ThrTrp: 0.598 ± 0.025	1.455ThrTyr: 1.455 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	5.954ValAla: 5.954 ± 0.067	0.756ValCys: 0.756 ± 0.027	3.734ValAsp: 3.734 ± 0.069	3.919ValGlu: 3.919 ± 0.072	2.518ValPhe: 2.518 ± 0.055	4.832ValGly: 4.832 ± 0.078	1.164ValHis: 1.164 ± 0.035	4.894ValIle: 4.894 ± 0.08	3.283ValLys: 3.283 ± 0.059	6.835ValLeu: 6.835 ± 0.096	2.07ValMet: 2.07 ± 0.037	3.168ValAsn: 3.168 ± 0.064	2.636ValPro: 2.636 ± 0.057	2.261ValGln: 2.261 ± 0.043	3.253ValArg: 3.253 ± 0.059	4.678ValSer: 4.678 ± 0.077	4.063ValThr: 4.063 ± 0.088	5.087ValVal: 5.087 ± 0.071	0.803ValTrp: 0.803 ± 0.028	1.851ValTyr: 1.851 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.856TrpAla: 0.856 ± 0.027	0.179TrpCys: 0.179 ± 0.013	0.567TrpAsp: 0.567 ± 0.024	0.498TrpGlu: 0.498 ± 0.023	0.582TrpPhe: 0.582 ± 0.026	0.83TrpGly: 0.83 ± 0.036	0.378TrpHis: 0.378 ± 0.018	0.817TrpIle: 0.817 ± 0.031	0.503TrpLys: 0.503 ± 0.019	2.006TrpLeu: 2.006 ± 0.055	0.4TrpMet: 0.4 ± 0.02	0.482TrpAsn: 0.482 ± 0.025	0.536TrpPro: 0.536 ± 0.027	1.037TrpGln: 1.037 ± 0.035	0.797TrpArg: 0.797 ± 0.033	0.813TrpSer: 0.813 ± 0.037	0.474TrpThr: 0.474 ± 0.022	0.817TrpVal: 0.817 ± 0.028	0.22TrpTrp: 0.22 ± 0.015	0.355TrpTyr: 0.355 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.416TyrAla: 2.416 ± 0.047	0.433TyrCys: 0.433 ± 0.02	1.585TyrAsp: 1.585 ± 0.038	1.197TyrGlu: 1.197 ± 0.039	1.27TyrPhe: 1.27 ± 0.036	2.368TyrGly: 2.368 ± 0.053	0.756TyrHis: 0.756 ± 0.027	1.792TyrIle: 1.792 ± 0.049	1.101TyrLys: 1.101 ± 0.034	3.503TyrLeu: 3.503 ± 0.06	0.646TyrMet: 0.646 ± 0.026	1.215TyrAsn: 1.215 ± 0.037	1.53TyrPro: 1.53 ± 0.039	2.035TyrGln: 2.035 ± 0.051	1.963TyrArg: 1.963 ± 0.053	2.082TyrSer: 2.082 ± 0.048	1.458TyrThr: 1.458 ± 0.034	1.738TyrVal: 1.738 ± 0.04	0.482TyrTrp: 0.482 ± 0.022	1.095TyrTyr: 1.095 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3326 proteins (1068109 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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