Sclerotinia nivalis victorivirus 1
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A192ABA0|A0A192ABA0_9VIRU Coat protein OS=Sclerotinia nivalis victorivirus 1 OX=1859161 PE=4 SV=1
MM1 pKa = 6.39 TTVIRR6 pKa = 11.84 NSFLASVLASPRR18 pKa = 11.84 GGYY21 pKa = 9.18 IEE23 pKa = 5.12 NDD25 pKa = 2.75 NTYY28 pKa = 10.67 RR29 pKa = 11.84 RR30 pKa = 11.84 YY31 pKa = 10.13 KK32 pKa = 10.61 SNVRR36 pKa = 11.84 TSSTIGGNEE45 pKa = 4.15 DD46 pKa = 3.19 SRR48 pKa = 11.84 LVQILYY54 pKa = 10.31 EE55 pKa = 4.16 VGRR58 pKa = 11.84 SKK60 pKa = 11.0 NSKK63 pKa = 9.32 GRR65 pKa = 11.84 ALARR69 pKa = 11.84 PGEE72 pKa = 4.51 DD73 pKa = 4.0 DD74 pKa = 3.94 PLIEE78 pKa = 5.42 AGYY81 pKa = 7.58 PTANALAEE89 pKa = 4.31 DD90 pKa = 4.43 FVGLSKK96 pKa = 11.09 KK97 pKa = 8.47 FTNFSATFEE106 pKa = 4.39 FSSHH110 pKa = 4.97 AAIVEE115 pKa = 3.99 RR116 pKa = 11.84 LARR119 pKa = 11.84 GLAASSIFDD128 pKa = 4.45 DD129 pKa = 4.23 VDD131 pKa = 4.17 SSDD134 pKa = 3.61 LRR136 pKa = 11.84 AGRR139 pKa = 11.84 TLVINALGTYY149 pKa = 9.44 DD150 pKa = 4.16 GPVNSLTSSVFIPRR164 pKa = 11.84 LVNSAITGDD173 pKa = 3.97 VFSVLANAAAGEE185 pKa = 4.22 GAEE188 pKa = 4.13 IATDD192 pKa = 4.16 IIEE195 pKa = 4.7 LDD197 pKa = 3.3 ALTRR201 pKa = 11.84 QPIVPEE207 pKa = 4.2 VEE209 pKa = 3.75 ATALSRR215 pKa = 11.84 AIVDD219 pKa = 3.5 ALRR222 pKa = 11.84 ILGANMIACDD232 pKa = 3.89 QGPLFALALTRR243 pKa = 11.84 GLHH246 pKa = 5.82 KK247 pKa = 10.54 VLTVVGHH254 pKa = 5.34 TDD256 pKa = 2.95 EE257 pKa = 5.08 GAVSRR262 pKa = 11.84 DD263 pKa = 3.37 LLRR266 pKa = 11.84 CGSFSVPFGDD276 pKa = 2.61 IHH278 pKa = 7.98 YY279 pKa = 10.37 GLEE282 pKa = 4.29 PYY284 pKa = 10.67 AGIPALSTNSSYY296 pKa = 11.14 GRR298 pKa = 11.84 AAYY301 pKa = 10.14 VDD303 pKa = 3.91 SLALSTAALVAHH315 pKa = 7.18 CDD317 pKa = 3.1 PGQIYY322 pKa = 10.24 DD323 pKa = 4.17 GRR325 pKa = 11.84 WLPTFFSGSSSTDD338 pKa = 2.77 TDD340 pKa = 3.31 GRR342 pKa = 11.84 PGDD345 pKa = 4.06 SLDD348 pKa = 3.64 PTDD351 pKa = 5.98 AMTLRR356 pKa = 11.84 NRR358 pKa = 11.84 AQLLSDD364 pKa = 4.16 LPKK367 pKa = 10.6 FSLSYY372 pKa = 10.61 IPALAKK378 pKa = 10.65 LFAAEE383 pKa = 4.38 GNPAIAIAAFNGASSQLSRR402 pKa = 11.84 TTRR405 pKa = 11.84 HH406 pKa = 5.84 LKK408 pKa = 9.56 FASVSPWFWIEE419 pKa = 3.71 PTSLIPHH426 pKa = 6.98 DD427 pKa = 4.16 FVGSVAEE434 pKa = 4.21 HH435 pKa = 6.68 EE436 pKa = 4.87 GFASYY441 pKa = 10.7 GGRR444 pKa = 11.84 DD445 pKa = 3.52 STRR448 pKa = 11.84 SRR450 pKa = 11.84 HH451 pKa = 4.49 AWEE454 pKa = 5.9 DD455 pKa = 3.34 IQASGVNDD463 pKa = 3.74 TAFSAYY469 pKa = 9.58 HH470 pKa = 5.77 ALFRR474 pKa = 11.84 GARR477 pKa = 11.84 ACWFFLHH484 pKa = 6.71 WLGNPANGLGSISVRR499 pKa = 11.84 QLDD502 pKa = 4.15 PNAIIHH508 pKa = 6.61 PGPCTAHH515 pKa = 4.78 QQVRR519 pKa = 11.84 DD520 pKa = 3.62 RR521 pKa = 11.84 VEE523 pKa = 5.12 ASLPWTDD530 pKa = 3.99 YY531 pKa = 11.03 LWTRR535 pKa = 11.84 GQSPFPAPGEE545 pKa = 3.88 MLNISGTVGFFVKK558 pKa = 10.2 HH559 pKa = 4.56 YY560 pKa = 9.25 TFDD563 pKa = 4.77 DD564 pKa = 3.78 EE565 pKa = 4.83 GLPTVEE571 pKa = 4.98 HH572 pKa = 6.43 VPASHH577 pKa = 6.75 EE578 pKa = 4.13 FLDD581 pKa = 4.13 TTVTLHH587 pKa = 5.32 VGRR590 pKa = 11.84 PIGLSIGASNTPASDD605 pKa = 2.87 ARR607 pKa = 11.84 RR608 pKa = 11.84 ARR610 pKa = 11.84 TRR612 pKa = 11.84 ATRR615 pKa = 11.84 EE616 pKa = 3.46 LAAASARR623 pKa = 11.84 AAVFGRR629 pKa = 11.84 PDD631 pKa = 3.39 VSEE634 pKa = 4.32 MPTLTTAPVLRR645 pKa = 11.84 SRR647 pKa = 11.84 NPTLPTHH654 pKa = 6.11 ITPNDD659 pKa = 3.78 TPGGTDD665 pKa = 2.98 VTHH668 pKa = 7.51 RR669 pKa = 11.84 SRR671 pKa = 11.84 HH672 pKa = 4.79 QGVGGADD679 pKa = 3.05 ASDD682 pKa = 3.77 RR683 pKa = 11.84 LPAGEE688 pKa = 4.26 PRR690 pKa = 11.84 IPVPQHH696 pKa = 4.65 QPVRR700 pKa = 11.84 YY701 pKa = 7.85 PQVPRR706 pKa = 11.84 PAGNQPGGGGPGIPPAPPGPGGPNGDD732 pKa = 5.15 DD733 pKa = 5.05 DD734 pKa = 6.67 DD735 pKa = 5.44 NDD737 pKa = 3.58 PPAPVNPPEE746 pKa = 4.98 GGPPTPPAGAAPPNGPAPII765 pKa = 4.49
Molecular weight: 80.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.622
IPC2_protein 5.461
IPC_protein 5.525
Toseland 5.957
ProMoST 5.855
Dawson 5.753
Bjellqvist 5.779
Wikipedia 5.753
Rodwell 5.728
Grimsley 6.148
Solomon 5.753
Lehninger 5.741
Nozaki 6.008
DTASelect 6.211
Thurlkill 6.186
EMBOSS 6.148
Sillero 6.109
Patrickios 4.24
IPC_peptide 5.766
IPC2_peptide 6.122
IPC2.peptide.svr19 6.014
Protein with the highest isoelectric point:
>tr|A0A192ABA0|A0A192ABA0_9VIRU Coat protein OS=Sclerotinia nivalis victorivirus 1 OX=1859161 PE=4 SV=1
MM1 pKa = 7.15 SAFVEE6 pKa = 4.35 RR7 pKa = 11.84 ASAYY11 pKa = 10.24 GVVGDD16 pKa = 4.29 YY17 pKa = 11.1 LLSLRR22 pKa = 11.84 SYY24 pKa = 10.91 FPDD27 pKa = 3.16 SFKK30 pKa = 11.13 GYY32 pKa = 10.17 GQTTFVNDD40 pKa = 3.72 LVRR43 pKa = 11.84 LQSSTPTLKK52 pKa = 9.83 TKK54 pKa = 10.43 HH55 pKa = 6.39 ALLPAAVSLLALPFPLQVPLTLSDD79 pKa = 3.39 ILVLAKK85 pKa = 10.12 RR86 pKa = 11.84 ASGVPFDD93 pKa = 4.48 YY94 pKa = 10.81 VHH96 pKa = 6.46 SARR99 pKa = 11.84 WSDD102 pKa = 3.61 SPLISSEE109 pKa = 3.76 YY110 pKa = 9.68 RR111 pKa = 11.84 GRR113 pKa = 11.84 DD114 pKa = 3.53 YY115 pKa = 11.03 MVLCTNALFLRR126 pKa = 11.84 NCFPVKK132 pKa = 9.93 KK133 pKa = 9.65 HH134 pKa = 5.64 ASAYY138 pKa = 9.65 SKK140 pKa = 11.42 VNVSLPDD147 pKa = 3.74 LAVSLCKK154 pKa = 10.32 LGHH157 pKa = 5.15 TRR159 pKa = 11.84 SLIGMIFRR167 pKa = 11.84 CAGLLHH173 pKa = 7.17 EE174 pKa = 5.06 DD175 pKa = 3.36 AFMNALIYY183 pKa = 10.36 GYY185 pKa = 10.45 ALSYY189 pKa = 10.99 HH190 pKa = 6.63 FGASGWDD197 pKa = 3.52 IACHH201 pKa = 6.59 FILNPKK207 pKa = 8.26 IAKK210 pKa = 9.28 NVSVSLKK217 pKa = 10.77 ALGANTTRR225 pKa = 11.84 EE226 pKa = 3.87 GALLVEE232 pKa = 5.02 ADD234 pKa = 3.82 TLQGRR239 pKa = 11.84 LAAPVDD245 pKa = 3.22 IDD247 pKa = 3.21 AQARR251 pKa = 11.84 YY252 pKa = 9.47 RR253 pKa = 11.84 CDD255 pKa = 3.09 PVAVAKK261 pKa = 10.55 SVIPYY266 pKa = 9.63 SDD268 pKa = 3.81 EE269 pKa = 3.79 LRR271 pKa = 11.84 THH273 pKa = 6.16 IRR275 pKa = 11.84 AILLDD280 pKa = 3.52 EE281 pKa = 4.41 TRR283 pKa = 11.84 DD284 pKa = 3.91 RR285 pKa = 11.84 EE286 pKa = 4.41 CVLPNLHH293 pKa = 6.38 SWWSARR299 pKa = 11.84 WLWCVNGSQTTKK311 pKa = 10.36 SSKK314 pKa = 11.29 DD315 pKa = 3.11 MGLDD319 pKa = 3.4 PKK321 pKa = 11.11 ANRR324 pKa = 11.84 ATHH327 pKa = 6.41 DD328 pKa = 3.17 QDD330 pKa = 3.5 FRR332 pKa = 11.84 RR333 pKa = 11.84 MASEE337 pKa = 3.9 ALKK340 pKa = 10.74 SEE342 pKa = 4.87 PLTTWDD348 pKa = 3.29 GTTSVSKK355 pKa = 10.59 SIKK358 pKa = 10.22 LEE360 pKa = 4.04 NGKK363 pKa = 9.26 EE364 pKa = 3.79 RR365 pKa = 11.84 AIFACDD371 pKa = 3.22 TNSYY375 pKa = 10.54 FAFSWLLSSAEE386 pKa = 4.08 KK387 pKa = 9.61 VWRR390 pKa = 11.84 GKK392 pKa = 10.39 RR393 pKa = 11.84 VILDD397 pKa = 3.66 PGVGGHH403 pKa = 7.04 LGIAKK408 pKa = 9.74 RR409 pKa = 11.84 VQGAQRR415 pKa = 11.84 GGGVNLMLDD424 pKa = 3.48 YY425 pKa = 11.52 DD426 pKa = 4.57 DD427 pKa = 6.33 FNSQHH432 pKa = 6.18 ATPVMQCVIDD442 pKa = 3.92 EE443 pKa = 4.39 LCNIFSCPDD452 pKa = 3.05 WYY454 pKa = 10.95 RR455 pKa = 11.84 EE456 pKa = 3.89 LLINSLDD463 pKa = 3.52 KK464 pKa = 11.0 MFINIDD470 pKa = 3.45 GAPQRR475 pKa = 11.84 VVGTLMSGHH484 pKa = 7.12 RR485 pKa = 11.84 GTTFFNSVLNAAYY498 pKa = 9.5 IRR500 pKa = 11.84 LAVGASAFDD509 pKa = 3.79 SLISLHH515 pKa = 6.98 AGDD518 pKa = 5.56 DD519 pKa = 3.74 VYY521 pKa = 11.1 IRR523 pKa = 11.84 CNTLSDD529 pKa = 4.29 CDD531 pKa = 4.37 SILNNTRR538 pKa = 11.84 RR539 pKa = 11.84 VGCRR543 pKa = 11.84 MNPSKK548 pKa = 10.84 QSIGFVGAEE557 pKa = 4.07 FLRR560 pKa = 11.84 VGIGTNAAYY569 pKa = 9.93 GYY571 pKa = 9.27 LARR574 pKa = 11.84 GIAGFVSGNWVTQDD588 pKa = 3.17 ILAPDD593 pKa = 4.3 DD594 pKa = 4.43 ALVSSISACRR604 pKa = 11.84 TLINRR609 pKa = 11.84 SGCASLALLLSRR621 pKa = 11.84 SLRR624 pKa = 11.84 YY625 pKa = 8.31 TSGFSHH631 pKa = 6.75 RR632 pKa = 11.84 VLRR635 pKa = 11.84 SLFSGEE641 pKa = 4.13 SALEE645 pKa = 3.82 GSPVYY650 pKa = 10.73 NVDD653 pKa = 3.38 YY654 pKa = 10.79 QIRR657 pKa = 11.84 NYY659 pKa = 10.64 KK660 pKa = 8.16 MNRR663 pKa = 11.84 VTPDD667 pKa = 3.88 SIPVPSNWARR677 pKa = 11.84 HH678 pKa = 4.78 ASIDD682 pKa = 3.78 YY683 pKa = 7.64 LTSHH687 pKa = 7.05 LSEE690 pKa = 5.19 IEE692 pKa = 3.92 VTAINMVGVDD702 pKa = 4.06 ALNLLVTSSYY712 pKa = 11.7 SKK714 pKa = 10.65 GLNMKK719 pKa = 9.46 YY720 pKa = 9.67 RR721 pKa = 11.84 AALTRR726 pKa = 11.84 PTISRR731 pKa = 11.84 MTVRR735 pKa = 11.84 LARR738 pKa = 11.84 GFVSAATLLDD748 pKa = 3.17 KK749 pKa = 10.83 RR750 pKa = 11.84 LEE752 pKa = 4.38 RR753 pKa = 11.84 GCLSQYY759 pKa = 9.98 PVVRR763 pKa = 11.84 LFEE766 pKa = 4.15 NRR768 pKa = 11.84 LDD770 pKa = 3.94 HH771 pKa = 7.2 NSLRR775 pKa = 11.84 EE776 pKa = 4.23 LITLAGGNPAARR788 pKa = 11.84 DD789 pKa = 3.55 LRR791 pKa = 11.84 EE792 pKa = 3.83 EE793 pKa = 4.1 AFGRR797 pKa = 11.84 TSLSKK802 pKa = 10.99 NIIGYY807 pKa = 9.82 LPFGDD812 pKa = 3.9 AASLSKK818 pKa = 10.19 RR819 pKa = 11.84 TTAGNIFTLYY829 pKa = 10.56 NVYY832 pKa = 8.82 TT833 pKa = 3.96
Molecular weight: 91.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.318
IPC2_protein 8.317
IPC_protein 8.17
Toseland 8.112
ProMoST 8.682
Dawson 8.916
Bjellqvist 9.107
Wikipedia 8.96
Rodwell 8.96
Grimsley 8.2
Solomon 8.975
Lehninger 8.946
Nozaki 9.209
DTASelect 8.887
Thurlkill 8.931
EMBOSS 9.063
Sillero 9.194
Patrickios 4.329
IPC_peptide 8.96
IPC2_peptide 8.126
IPC2.peptide.svr19 8.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1598
765
833
799.0
86.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.513 ± 0.856
1.439 ± 0.537
5.945 ± 0.225
3.379 ± 0.282
3.942 ± 0.075
8.073 ± 0.916
2.441 ± 0.208
4.819 ± 0.077
2.503 ± 0.818
10.013 ± 1.305
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.252 ± 0.409
4.38 ± 0.314
6.32 ± 1.936
2.003 ± 0.061
7.009 ± 0.145
9.199 ± 0.48
6.07 ± 0.497
6.446 ± 0.296
1.252 ± 0.141
3.004 ± 0.535
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here