Streptococcus phage IPP64

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SFF2|A0A1S5SFF2_9CAUD Uncharacterized protein OS=Streptococcus phage IPP64 OX=1916200 GN=IPP64_00002 PE=4 SV=1
MM1 pKa = 8.39LMFNYY6 pKa = 10.01DD7 pKa = 3.94RR8 pKa = 11.84DD9 pKa = 3.49IMQPPEE15 pKa = 3.98EE16 pKa = 4.61RR17 pKa = 11.84EE18 pKa = 4.03EE19 pKa = 4.62LDD21 pKa = 3.41PADD24 pKa = 4.33WIFSAGQWIYY34 pKa = 11.59VGDD37 pKa = 4.05CC38 pKa = 3.39

Molecular weight: 4.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SFK0|A0A1S5SFK0_9CAUD Putative head morphogenesis protein OS=Streptococcus phage IPP64 OX=1916200 GN=IPP64_00043 PE=4 SV=1
MM1 pKa = 7.55FIWDD5 pKa = 3.59WVSIAFGWLVFLLLIFIIMAVISGIIKK32 pKa = 9.8GVKK35 pKa = 9.76KK36 pKa = 9.73GTEE39 pKa = 3.21KK40 pKa = 10.25WKK42 pKa = 10.77NGKK45 pKa = 9.27KK46 pKa = 9.92DD47 pKa = 3.61LKK49 pKa = 11.23KK50 pKa = 10.1NTTNN54 pKa = 3.39

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

12541

32

2124

187.2

21.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.834 ± 0.587

0.67 ± 0.114

6.483 ± 0.286

8.101 ± 0.496

4.194 ± 0.226

6.57 ± 0.516

1.372 ± 0.188

6.507 ± 0.328

8.133 ± 0.361

8.141 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.23

5.35 ± 0.235

2.544 ± 0.221

4.282 ± 0.277

4.88 ± 0.308

6.044 ± 0.356

6.044 ± 0.382

6.443 ± 0.222

1.372 ± 0.142

3.636 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski