Amino acid dipepetide frequency for Streptococcus phage IPP64

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.632AlaAla: 2.632 ± 0.779
0.239AlaCys: 0.239 ± 0.132
6.3AlaAsp: 6.3 ± 0.763
6.3AlaGlu: 6.3 ± 0.654
2.711AlaPhe: 2.711 ± 0.613
5.263AlaGly: 5.263 ± 0.957
0.558AlaHis: 0.558 ± 0.173
4.545AlaIle: 4.545 ± 0.853
4.785AlaLys: 4.785 ± 0.682
6.459AlaLeu: 6.459 ± 0.867
2.313AlaMet: 2.313 ± 0.397
3.429AlaAsn: 3.429 ± 0.617
1.675AlaPro: 1.675 ± 0.343
3.03AlaGln: 3.03 ± 0.456
3.03AlaArg: 3.03 ± 0.466
2.313AlaSer: 2.313 ± 0.652
4.545AlaThr: 4.545 ± 0.64
5.024AlaVal: 5.024 ± 0.841
1.356AlaTrp: 1.356 ± 0.386
1.834AlaTyr: 1.834 ± 0.305
0.0AlaXaa: 0.0 ± 0.0
Cys
0.239CysAla: 0.239 ± 0.11
0.159CysCys: 0.159 ± 0.117
0.478CysAsp: 0.478 ± 0.19
0.638CysGlu: 0.638 ± 0.238
0.239CysPhe: 0.239 ± 0.138
0.558CysGly: 0.558 ± 0.238
0.159CysHis: 0.159 ± 0.104
0.399CysIle: 0.399 ± 0.197
0.718CysLys: 0.718 ± 0.207
0.558CysLeu: 0.558 ± 0.206
0.08CysMet: 0.08 ± 0.089
0.159CysAsn: 0.159 ± 0.163
0.159CysPro: 0.159 ± 0.11
0.399CysGln: 0.399 ± 0.166
0.399CysArg: 0.399 ± 0.162
0.399CysSer: 0.399 ± 0.179
0.159CysThr: 0.159 ± 0.115
0.08CysVal: 0.08 ± 0.081
0.239CysTrp: 0.239 ± 0.116
0.478CysTyr: 0.478 ± 0.171
0.0CysXaa: 0.0 ± 0.0
Asp
3.987AspAla: 3.987 ± 0.532
0.558AspCys: 0.558 ± 0.223
3.03AspAsp: 3.03 ± 0.679
4.306AspGlu: 4.306 ± 0.877
3.19AspPhe: 3.19 ± 0.495
5.263AspGly: 5.263 ± 0.623
0.399AspHis: 0.399 ± 0.204
5.662AspIle: 5.662 ± 0.608
5.024AspLys: 5.024 ± 0.707
5.423AspLeu: 5.423 ± 0.66
2.073AspMet: 2.073 ± 0.354
2.711AspAsn: 2.711 ± 0.478
1.994AspPro: 1.994 ± 0.472
1.914AspGln: 1.914 ± 0.393
3.349AspArg: 3.349 ± 0.615
3.429AspSer: 3.429 ± 0.478
3.828AspThr: 3.828 ± 0.416
3.589AspVal: 3.589 ± 0.529
1.834AspTrp: 1.834 ± 0.36
3.27AspTyr: 3.27 ± 0.632
0.0AspXaa: 0.0 ± 0.0
Glu
5.821GluAla: 5.821 ± 0.899
0.558GluCys: 0.558 ± 0.146
4.306GluAsp: 4.306 ± 0.643
6.38GluGlu: 6.38 ± 0.981
3.907GluPhe: 3.907 ± 0.653
3.509GluGly: 3.509 ± 0.563
1.116GluHis: 1.116 ± 0.308
6.22GluIle: 6.22 ± 0.692
6.061GluLys: 6.061 ± 1.022
8.772GluLeu: 8.772 ± 0.995
1.754GluMet: 1.754 ± 0.418
5.423GluAsn: 5.423 ± 0.605
1.914GluPro: 1.914 ± 0.405
3.349GluGln: 3.349 ± 0.514
4.785GluArg: 4.785 ± 0.586
4.785GluSer: 4.785 ± 0.608
3.748GluThr: 3.748 ± 0.553
5.104GluVal: 5.104 ± 0.582
0.877GluTrp: 0.877 ± 0.249
2.552GluTyr: 2.552 ± 0.404
0.0GluXaa: 0.0 ± 0.0
Phe
2.552PheAla: 2.552 ± 0.544
0.319PheCys: 0.319 ± 0.172
4.705PheAsp: 4.705 ± 0.523
4.067PheGlu: 4.067 ± 0.679
1.515PhePhe: 1.515 ± 0.322
2.313PheGly: 2.313 ± 0.605
0.239PheHis: 0.239 ± 0.147
2.711PheIle: 2.711 ± 0.536
3.509PheLys: 3.509 ± 0.482
2.632PheLeu: 2.632 ± 0.39
1.037PheMet: 1.037 ± 0.307
2.392PheAsn: 2.392 ± 0.473
0.957PhePro: 0.957 ± 0.359
1.515PheGln: 1.515 ± 0.38
1.276PheArg: 1.276 ± 0.296
3.349PheSer: 3.349 ± 0.548
2.791PheThr: 2.791 ± 0.418
1.595PheVal: 1.595 ± 0.381
0.638PheTrp: 0.638 ± 0.231
1.834PheTyr: 1.834 ± 0.339
0.0PheXaa: 0.0 ± 0.0
Gly
3.11GlyAla: 3.11 ± 0.443
0.0GlyCys: 0.0 ± 0.0
3.589GlyAsp: 3.589 ± 0.53
5.263GlyGlu: 5.263 ± 0.744
2.871GlyPhe: 2.871 ± 0.576
4.785GlyGly: 4.785 ± 1.109
0.877GlyHis: 0.877 ± 0.199
4.067GlyIle: 4.067 ± 0.467
4.944GlyLys: 4.944 ± 0.504
5.662GlyLeu: 5.662 ± 0.926
1.754GlyMet: 1.754 ± 0.301
3.589GlyAsn: 3.589 ± 0.497
0.797GlyPro: 0.797 ± 0.248
3.987GlyGln: 3.987 ± 0.526
3.748GlyArg: 3.748 ± 0.517
4.386GlySer: 4.386 ± 0.678
2.871GlyThr: 2.871 ± 0.542
5.423GlyVal: 5.423 ± 0.66
0.957GlyTrp: 0.957 ± 0.408
3.03GlyTyr: 3.03 ± 0.424
0.0GlyXaa: 0.0 ± 0.0
His
0.877HisAla: 0.877 ± 0.273
0.08HisCys: 0.08 ± 0.074
1.116HisAsp: 1.116 ± 0.374
1.116HisGlu: 1.116 ± 0.288
0.718HisPhe: 0.718 ± 0.219
0.718HisGly: 0.718 ± 0.252
0.239HisHis: 0.239 ± 0.139
0.478HisIle: 0.478 ± 0.253
0.558HisLys: 0.558 ± 0.175
1.116HisLeu: 1.116 ± 0.253
0.159HisMet: 0.159 ± 0.113
0.877HisAsn: 0.877 ± 0.265
0.797HisPro: 0.797 ± 0.212
0.638HisGln: 0.638 ± 0.259
0.718HisArg: 0.718 ± 0.232
1.037HisSer: 1.037 ± 0.357
0.718HisThr: 0.718 ± 0.256
1.037HisVal: 1.037 ± 0.269
0.239HisTrp: 0.239 ± 0.137
0.478HisTyr: 0.478 ± 0.185
0.0HisXaa: 0.0 ± 0.0
Ile
5.981IleAla: 5.981 ± 0.752
0.478IleCys: 0.478 ± 0.151
3.907IleAsp: 3.907 ± 0.49
6.539IleGlu: 6.539 ± 0.64
2.951IlePhe: 2.951 ± 0.551
4.625IleGly: 4.625 ± 0.887
0.558IleHis: 0.558 ± 0.199
3.27IleIle: 3.27 ± 0.531
6.38IleLys: 6.38 ± 0.694
3.748IleLeu: 3.748 ± 0.489
1.196IleMet: 1.196 ± 0.349
3.907IleAsn: 3.907 ± 0.53
0.877IlePro: 0.877 ± 0.303
2.392IleGln: 2.392 ± 0.365
3.429IleArg: 3.429 ± 0.683
5.662IleSer: 5.662 ± 0.739
4.306IleThr: 4.306 ± 0.506
2.791IleVal: 2.791 ± 0.506
0.319IleTrp: 0.319 ± 0.154
1.754IleTyr: 1.754 ± 0.482
0.0IleXaa: 0.0 ± 0.0
Lys
5.343LysAla: 5.343 ± 0.648
0.718LysCys: 0.718 ± 0.245
5.662LysAsp: 5.662 ± 0.573
6.858LysGlu: 6.858 ± 0.716
2.552LysPhe: 2.552 ± 0.489
4.386LysGly: 4.386 ± 0.599
0.957LysHis: 0.957 ± 0.21
5.901LysIle: 5.901 ± 0.853
7.097LysLys: 7.097 ± 0.942
7.177LysLeu: 7.177 ± 0.81
2.711LysMet: 2.711 ± 0.45
4.386LysAsn: 4.386 ± 0.453
2.233LysPro: 2.233 ± 0.476
3.668LysGln: 3.668 ± 0.54
3.27LysArg: 3.27 ± 0.45
4.067LysSer: 4.067 ± 0.488
4.306LysThr: 4.306 ± 0.406
6.3LysVal: 6.3 ± 0.651
0.877LysTrp: 0.877 ± 0.303
2.871LysTyr: 2.871 ± 0.477
0.0LysXaa: 0.0 ± 0.0
Leu
6.539LeuAla: 6.539 ± 0.789
0.638LeuCys: 0.638 ± 0.281
6.619LeuAsp: 6.619 ± 0.731
7.257LeuGlu: 7.257 ± 0.887
2.552LeuPhe: 2.552 ± 0.476
5.981LeuGly: 5.981 ± 1.19
1.276LeuHis: 1.276 ± 0.277
4.864LeuIle: 4.864 ± 0.515
6.858LeuLys: 6.858 ± 0.668
7.257LeuLeu: 7.257 ± 1.022
1.914LeuMet: 1.914 ± 0.362
3.828LeuAsn: 3.828 ± 0.489
2.233LeuPro: 2.233 ± 0.51
3.03LeuGln: 3.03 ± 0.531
4.466LeuArg: 4.466 ± 0.518
5.662LeuSer: 5.662 ± 0.923
4.545LeuThr: 4.545 ± 0.705
3.19LeuVal: 3.19 ± 0.383
0.718LeuTrp: 0.718 ± 0.199
1.994LeuTyr: 1.994 ± 0.307
0.0LeuXaa: 0.0 ± 0.0
Met
2.153MetAla: 2.153 ± 0.492
0.0MetCys: 0.0 ± 0.0
1.435MetAsp: 1.435 ± 0.299
1.754MetGlu: 1.754 ± 0.397
1.116MetPhe: 1.116 ± 0.265
1.276MetGly: 1.276 ± 0.411
0.319MetHis: 0.319 ± 0.212
1.754MetIle: 1.754 ± 0.416
2.472MetLys: 2.472 ± 0.476
1.754MetLeu: 1.754 ± 0.31
0.478MetMet: 0.478 ± 0.18
1.515MetAsn: 1.515 ± 0.434
0.797MetPro: 0.797 ± 0.255
0.957MetGln: 0.957 ± 0.4
1.196MetArg: 1.196 ± 0.253
1.116MetSer: 1.116 ± 0.29
1.994MetThr: 1.994 ± 0.475
1.037MetVal: 1.037 ± 0.248
0.159MetTrp: 0.159 ± 0.11
0.718MetTyr: 0.718 ± 0.183
0.0MetXaa: 0.0 ± 0.0
Asn
4.625AsnAla: 4.625 ± 0.859
0.239AsnCys: 0.239 ± 0.114
2.951AsnAsp: 2.951 ± 0.462
3.668AsnGlu: 3.668 ± 0.644
1.754AsnPhe: 1.754 ± 0.334
4.306AsnGly: 4.306 ± 0.536
1.276AsnHis: 1.276 ± 0.283
3.03AsnIle: 3.03 ± 0.543
4.067AsnLys: 4.067 ± 0.509
4.147AsnLeu: 4.147 ± 0.632
1.037AsnMet: 1.037 ± 0.311
2.313AsnAsn: 2.313 ± 0.436
1.675AsnPro: 1.675 ± 0.309
2.552AsnGln: 2.552 ± 0.502
2.233AsnArg: 2.233 ± 0.512
3.828AsnSer: 3.828 ± 0.628
3.748AsnThr: 3.748 ± 0.617
3.349AsnVal: 3.349 ± 0.444
0.877AsnTrp: 0.877 ± 0.183
1.834AsnTyr: 1.834 ± 0.335
0.0AsnXaa: 0.0 ± 0.0
Pro
1.994ProAla: 1.994 ± 0.38
0.159ProCys: 0.159 ± 0.116
2.313ProAsp: 2.313 ± 0.369
2.951ProGlu: 2.951 ± 0.443
1.196ProPhe: 1.196 ± 0.412
0.957ProGly: 0.957 ± 0.235
0.478ProHis: 0.478 ± 0.177
1.595ProIle: 1.595 ± 0.483
2.472ProLys: 2.472 ± 0.359
1.276ProLeu: 1.276 ± 0.302
0.478ProMet: 0.478 ± 0.244
1.435ProAsn: 1.435 ± 0.476
0.718ProPro: 0.718 ± 0.289
1.037ProGln: 1.037 ± 0.282
1.435ProArg: 1.435 ± 0.283
0.877ProSer: 0.877 ± 0.316
0.797ProThr: 0.797 ± 0.23
1.515ProVal: 1.515 ± 0.294
0.478ProTrp: 0.478 ± 0.173
1.276ProTyr: 1.276 ± 0.41
0.0ProXaa: 0.0 ± 0.0
Gln
3.429GlnAla: 3.429 ± 0.492
0.319GlnCys: 0.319 ± 0.166
1.675GlnAsp: 1.675 ± 0.301
4.386GlnGlu: 4.386 ± 0.612
1.037GlnPhe: 1.037 ± 0.274
2.233GlnGly: 2.233 ± 0.469
0.478GlnHis: 0.478 ± 0.198
3.509GlnIle: 3.509 ± 0.511
3.27GlnLys: 3.27 ± 0.488
3.03GlnLeu: 3.03 ± 0.403
0.718GlnMet: 0.718 ± 0.21
1.914GlnAsn: 1.914 ± 0.366
1.116GlnPro: 1.116 ± 0.316
1.754GlnGln: 1.754 ± 0.427
2.313GlnArg: 2.313 ± 0.374
3.03GlnSer: 3.03 ± 0.43
2.711GlnThr: 2.711 ± 0.502
4.306GlnVal: 4.306 ± 0.487
0.478GlnTrp: 0.478 ± 0.166
1.116GlnTyr: 1.116 ± 0.322
0.0GlnXaa: 0.0 ± 0.0
Arg
2.951ArgAla: 2.951 ± 0.425
0.319ArgCys: 0.319 ± 0.124
2.552ArgAsp: 2.552 ± 0.451
3.11ArgGlu: 3.11 ± 0.53
2.073ArgPhe: 2.073 ± 0.473
2.791ArgGly: 2.791 ± 0.481
0.478ArgHis: 0.478 ± 0.177
3.27ArgIle: 3.27 ± 0.502
3.668ArgLys: 3.668 ± 0.581
5.343ArgLeu: 5.343 ± 0.629
2.153ArgMet: 2.153 ± 0.383
2.791ArgAsn: 2.791 ± 0.584
1.196ArgPro: 1.196 ± 0.284
2.791ArgGln: 2.791 ± 0.483
2.871ArgArg: 2.871 ± 0.589
1.994ArgSer: 1.994 ± 0.391
3.27ArgThr: 3.27 ± 0.638
3.27ArgVal: 3.27 ± 0.464
0.399ArgTrp: 0.399 ± 0.147
1.515ArgTyr: 1.515 ± 0.35
0.0ArgXaa: 0.0 ± 0.0
Ser
4.705SerAla: 4.705 ± 0.912
0.478SerCys: 0.478 ± 0.206
3.828SerAsp: 3.828 ± 0.565
3.509SerGlu: 3.509 ± 0.528
2.392SerPhe: 2.392 ± 0.401
5.263SerGly: 5.263 ± 0.772
1.276SerHis: 1.276 ± 0.365
3.987SerIle: 3.987 ± 0.538
4.545SerLys: 4.545 ± 0.47
4.705SerLeu: 4.705 ± 0.591
1.276SerMet: 1.276 ± 0.338
3.19SerAsn: 3.19 ± 0.492
1.435SerPro: 1.435 ± 0.277
2.711SerGln: 2.711 ± 0.418
3.589SerArg: 3.589 ± 0.735
2.871SerSer: 2.871 ± 0.837
3.589SerThr: 3.589 ± 0.34
3.828SerVal: 3.828 ± 0.733
1.037SerTrp: 1.037 ± 0.341
2.233SerTyr: 2.233 ± 0.467
0.0SerXaa: 0.0 ± 0.0
Thr
4.625ThrAla: 4.625 ± 0.759
0.239ThrCys: 0.239 ± 0.137
3.987ThrAsp: 3.987 ± 0.598
3.987ThrGlu: 3.987 ± 0.52
3.509ThrPhe: 3.509 ± 0.746
3.907ThrGly: 3.907 ± 0.754
0.877ThrHis: 0.877 ± 0.317
3.748ThrIle: 3.748 ± 0.613
4.067ThrLys: 4.067 ± 0.756
4.864ThrLeu: 4.864 ± 0.603
0.718ThrMet: 0.718 ± 0.203
3.27ThrAsn: 3.27 ± 0.368
1.196ThrPro: 1.196 ± 0.348
2.871ThrGln: 2.871 ± 0.522
1.675ThrArg: 1.675 ± 0.355
4.226ThrSer: 4.226 ± 0.599
4.705ThrThr: 4.705 ± 0.772
4.466ThrVal: 4.466 ± 0.822
1.116ThrTrp: 1.116 ± 0.286
2.392ThrTyr: 2.392 ± 0.536
0.0ThrXaa: 0.0 ± 0.0
Val
4.466ValAla: 4.466 ± 0.559
0.319ValCys: 0.319 ± 0.154
3.589ValAsp: 3.589 ± 0.563
5.502ValGlu: 5.502 ± 0.659
2.951ValPhe: 2.951 ± 0.448
4.944ValGly: 4.944 ± 0.693
1.037ValHis: 1.037 ± 0.367
2.871ValIle: 2.871 ± 0.448
5.981ValLys: 5.981 ± 0.549
3.668ValLeu: 3.668 ± 0.465
1.196ValMet: 1.196 ± 0.341
4.226ValAsn: 4.226 ± 0.679
2.073ValPro: 2.073 ± 0.286
1.435ValGln: 1.435 ± 0.388
2.392ValArg: 2.392 ± 0.288
4.864ValSer: 4.864 ± 0.65
4.864ValThr: 4.864 ± 0.528
4.386ValVal: 4.386 ± 0.694
0.558ValTrp: 0.558 ± 0.176
3.11ValTyr: 3.11 ± 0.564
0.0ValXaa: 0.0 ± 0.0
Trp
1.196TrpAla: 1.196 ± 0.334
0.159TrpCys: 0.159 ± 0.096
0.558TrpAsp: 0.558 ± 0.297
0.718TrpGlu: 0.718 ± 0.264
1.037TrpPhe: 1.037 ± 0.394
0.638TrpGly: 0.638 ± 0.187
0.319TrpHis: 0.319 ± 0.162
0.718TrpIle: 0.718 ± 0.246
1.276TrpLys: 1.276 ± 0.304
0.877TrpLeu: 0.877 ± 0.366
0.319TrpMet: 0.319 ± 0.131
0.957TrpAsn: 0.957 ± 0.298
0.08TrpPro: 0.08 ± 0.084
0.638TrpGln: 0.638 ± 0.263
0.638TrpArg: 0.638 ± 0.231
0.399TrpSer: 0.399 ± 0.185
0.797TrpThr: 0.797 ± 0.257
1.515TrpVal: 1.515 ± 0.3
0.159TrpTrp: 0.159 ± 0.095
0.718TrpTyr: 0.718 ± 0.48
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.116TyrAla: 1.116 ± 0.216
0.718TyrCys: 0.718 ± 0.269
2.233TyrAsp: 2.233 ± 0.373
2.711TyrGlu: 2.711 ± 0.492
1.675TyrPhe: 1.675 ± 0.372
1.994TyrGly: 1.994 ± 0.324
0.797TyrHis: 0.797 ± 0.246
2.472TyrIle: 2.472 ± 0.458
3.589TyrLys: 3.589 ± 0.631
2.951TyrLeu: 2.951 ± 0.481
0.558TyrMet: 0.558 ± 0.22
1.196TyrAsn: 1.196 ± 0.269
1.515TyrPro: 1.515 ± 0.358
2.153TyrGln: 2.153 ± 0.412
1.994TyrArg: 1.994 ± 0.42
2.153TyrSer: 2.153 ± 0.522
2.153TyrThr: 2.153 ± 0.416
2.632TyrVal: 2.632 ± 0.553
0.399TyrTrp: 0.399 ± 0.138
1.356TyrTyr: 1.356 ± 0.495
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (12541 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski