Chelatococcus asaccharovorans
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6659 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V3TT05|A0A2V3TT05_9RHIZ Uncharacterized protein OS=Chelatococcus asaccharovorans OX=28210 GN=C7450_1214 PE=4 SV=1
MM1 pKa = 7.44 ATTDD5 pKa = 3.63 AASSSLIPAHH15 pKa = 6.81 SPFSYY20 pKa = 10.73 NVGINYY26 pKa = 7.91 EE27 pKa = 4.08 SWDD30 pKa = 3.51 GGRR33 pKa = 11.84 VGRR36 pKa = 11.84 VIANDD41 pKa = 3.69 LDD43 pKa = 5.89 AITQNFKK50 pKa = 11.09 LIRR53 pKa = 11.84 TYY55 pKa = 11.15 HH56 pKa = 6.48 DD57 pKa = 3.43 AAVGTSDD64 pKa = 3.21 PTAPIIEE71 pKa = 4.31 PTQQEE76 pKa = 4.46 VISYY80 pKa = 9.26 VLAHH84 pKa = 6.99 PGLEE88 pKa = 3.99 LSMGTNNNALANGGYY103 pKa = 8.93 GAPWSPGLMVDD114 pKa = 5.25 PAYY117 pKa = 9.84 TDD119 pKa = 2.85 QWVAMIIEE127 pKa = 4.75 AFGSTNAVEE136 pKa = 5.02 EE137 pKa = 4.08 NLKK140 pKa = 10.18 MILLGNEE147 pKa = 3.83 LDD149 pKa = 4.51 ANGPPPGDD157 pKa = 3.51 PLFEE161 pKa = 4.26 TYY163 pKa = 10.76 YY164 pKa = 10.85 SVWIPTAFQNLQTSLANAGLGSIPISTTIANYY196 pKa = 8.14 GTGNVVSTSISSFIEE211 pKa = 4.55 TNWSASWNGGEE222 pKa = 5.26 PIVLYY227 pKa = 10.51 NQYY230 pKa = 9.2 TPDD233 pKa = 3.72 NGSSTDD239 pKa = 3.51 YY240 pKa = 11.1 SSVISYY246 pKa = 10.08 FEE248 pKa = 4.12 SLATTFDD255 pKa = 3.3 GALAPFIGEE264 pKa = 4.27 TGFSTLYY271 pKa = 9.71 GQPGQVTVYY280 pKa = 10.68 SQIFDD285 pKa = 3.76 WLNSQWDD292 pKa = 4.01 TGHH295 pKa = 5.92 KK296 pKa = 8.56 TVPLFAFDD304 pKa = 6.11 AFDD307 pKa = 5.55 QPAAWPDD314 pKa = 3.15 VEE316 pKa = 4.85 KK317 pKa = 11.17 YY318 pKa = 10.45 YY319 pKa = 11.14 GIYY322 pKa = 9.42 TDD324 pKa = 4.48 SGSLKK329 pKa = 10.14 PGLAAILPSWLTTATSTYY347 pKa = 10.83 VEE349 pKa = 4.23 GTAAAEE355 pKa = 4.06 RR356 pKa = 11.84 FFAKK360 pKa = 10.08 PGNDD364 pKa = 2.76 IVDD367 pKa = 4.18 PGLGDD372 pKa = 4.38 DD373 pKa = 4.05 VLALGGTSARR383 pKa = 11.84 YY384 pKa = 9.16 SVALSGAVALVTDD397 pKa = 4.8 LMTNSTDD404 pKa = 3.36 VVSNVQALEE413 pKa = 4.02 FADD416 pKa = 3.92 GTKK419 pKa = 10.87 ALFATDD425 pKa = 3.49 QTHH428 pKa = 6.49 IAALYY433 pKa = 9.33 EE434 pKa = 4.22 AMLGRR439 pKa = 11.84 DD440 pKa = 3.56 ADD442 pKa = 4.07 SAGLAFWASQSFSGTTFDD460 pKa = 4.83 AMASAFTDD468 pKa = 3.44 SAEE471 pKa = 4.01 YY472 pKa = 10.15 AARR475 pKa = 11.84 STLDD479 pKa = 3.28 DD480 pKa = 4.09 STWLAGLYY488 pKa = 10.62 DD489 pKa = 4.13 NLLHH493 pKa = 7.14 RR494 pKa = 11.84 AADD497 pKa = 4.06 SEE499 pKa = 4.42 GQAYY503 pKa = 8.54 WLAQRR508 pKa = 11.84 DD509 pKa = 3.77 AGVARR514 pKa = 11.84 HH515 pKa = 5.97 EE516 pKa = 4.31 IEE518 pKa = 4.03 TTFILSAEE526 pKa = 4.29 FTSAAHH532 pKa = 6.71 DD533 pKa = 4.42 LVDD536 pKa = 3.47 STQWMGLVGGTLPDD550 pKa = 3.68 LPAAHH555 pKa = 6.81 GPSSS559 pKa = 3.26
Molecular weight: 59.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.884
IPC_protein 3.91
Toseland 3.694
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.732
Grimsley 3.605
Solomon 3.897
Lehninger 3.859
Nozaki 4.012
DTASelect 4.253
Thurlkill 3.745
EMBOSS 3.846
Sillero 4.037
Patrickios 0.655
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.903
Protein with the highest isoelectric point:
>tr|A0A2V3UHC2|A0A2V3UHC2_9RHIZ Elongation factor Tu (Fragment) OS=Chelatococcus asaccharovorans OX=28210 GN=C7450_106425 PE=3 SV=1
MM1 pKa = 7.46 ARR3 pKa = 11.84 SQAVASGVGQPVSAQPASVLVGTAAFGAPLLVLALGHH40 pKa = 6.14 MLSNMLRR47 pKa = 11.84 TLPAIATDD55 pKa = 3.61 VMAVDD60 pKa = 4.12 IGVSAEE66 pKa = 3.95 ALASLTGAYY75 pKa = 9.71 HH76 pKa = 6.33 FAFAAGQIPVGVALDD91 pKa = 3.83 RR92 pKa = 11.84 YY93 pKa = 9.13 GVRR96 pKa = 11.84 TVSLTLFAIVTVGAALAAVVGGAAGFFLAQIVLGVGCCGMLLCPMTLAAKK146 pKa = 10.39 LLTPAKK152 pKa = 9.78 FGLWSGLIQGVGNSGMLLSASPLAWLIEE180 pKa = 4.13 MAGWRR185 pKa = 11.84 AGYY188 pKa = 9.71 GVAAIAGVVIAGLVFLLVPNTPPEE212 pKa = 4.39 RR213 pKa = 11.84 GAGHH217 pKa = 5.92 ATLRR221 pKa = 11.84 SEE223 pKa = 3.97 ARR225 pKa = 11.84 EE226 pKa = 3.9 VVQIGLSARR235 pKa = 11.84 MRR237 pKa = 11.84 GIIILALVSFAVMIGIRR254 pKa = 11.84 GLWGGPWLMDD264 pKa = 3.16 VKK266 pKa = 10.9 GLSRR270 pKa = 11.84 VEE272 pKa = 3.96 AGAVLMPLTIALVLGPIAFGMLDD295 pKa = 3.02 RR296 pKa = 11.84 RR297 pKa = 11.84 IGHH300 pKa = 6.21 RR301 pKa = 11.84 RR302 pKa = 11.84 ALIIAGHH309 pKa = 5.99 AVAGAMLLLLAMGGPAGTLSRR330 pKa = 11.84 LLGEE334 pKa = 4.15 MSLPVTFDD342 pKa = 3.13 TTVLFIFGATIAVQPLLFAMGRR364 pKa = 11.84 SIFTPDD370 pKa = 3.11 KK371 pKa = 10.77 AGKK374 pKa = 9.72 ALAAINFAFFAGAAIFQPVTGAVSAVWGTAGVIVFLGLLMLTCTLAFALMTEE426 pKa = 4.39 RR427 pKa = 11.84 RR428 pKa = 11.84 ATGGRR433 pKa = 3.5
Molecular weight: 44.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 9.443
IPC_protein 10.321
Toseland 10.511
ProMoST 10.526
Dawson 10.628
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.701
Grimsley 10.687
Solomon 10.76
Lehninger 10.73
Nozaki 10.54
DTASelect 10.365
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.57
Patrickios 10.438
IPC_peptide 10.76
IPC2_peptide 9.692
IPC2.peptide.svr19 8.669
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6659
0
6659
2107697
28
2242
316.5
34.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.115 ± 0.042
0.807 ± 0.009
5.553 ± 0.024
5.25 ± 0.026
3.739 ± 0.02
8.672 ± 0.027
2.024 ± 0.014
5.347 ± 0.019
2.893 ± 0.024
10.327 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.407 ± 0.012
2.407 ± 0.018
5.435 ± 0.023
2.911 ± 0.015
7.366 ± 0.031
5.344 ± 0.019
5.294 ± 0.017
7.652 ± 0.024
1.293 ± 0.011
2.163 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here