Alkalispirochaeta odontotermitis

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Spirochaetaceae; Alkalispirochaeta

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2732 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0E2U2|A0A0A0E2U2_9SPIO Uncharacterized protein OS=Alkalispirochaeta odontotermitis OX=1329640 GN=JY97_00340 PE=4 SV=1
MM1 pKa = 8.16AYY3 pKa = 8.37PQKK6 pKa = 10.35PITITASTTLDD17 pKa = 3.96PDD19 pKa = 3.78THH21 pKa = 7.74GMTTTKK27 pKa = 10.56LSAAAGLTVTLPDD40 pKa = 3.39ATGSGMVYY48 pKa = 10.25DD49 pKa = 4.56IYY51 pKa = 11.53VATTVTSNDD60 pKa = 3.59YY61 pKa = 10.61IIQVPDD67 pKa = 3.67ASNVIQGAVTISTDD81 pKa = 2.92IAGVSVPTAATSDD94 pKa = 4.08TITMNGSTTGGVIGSNLRR112 pKa = 11.84LVDD115 pKa = 3.63VAADD119 pKa = 3.59TWQVSGHH126 pKa = 6.93LISTGVEE133 pKa = 3.75ATPFSAAVSS142 pKa = 3.76

Molecular weight: 14.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0DYQ5|A0A0A0DYQ5_9SPIO 50S ribosomal protein L10 OS=Alkalispirochaeta odontotermitis OX=1329640 GN=rplJ PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84IKK11 pKa = 10.45RR12 pKa = 11.84ARR14 pKa = 11.84NHH16 pKa = 6.07GFLKK20 pKa = 10.62RR21 pKa = 11.84MSTKK25 pKa = 9.49QGRR28 pKa = 11.84RR29 pKa = 11.84IINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.52GRR39 pKa = 11.84VRR41 pKa = 11.84LAGG44 pKa = 3.53

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2732

0

2732

888817

38

4015

325.3

36.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.644 ± 0.056

1.097 ± 0.023

5.601 ± 0.049

6.323 ± 0.048

4.298 ± 0.035

7.54 ± 0.042

1.948 ± 0.022

7.126 ± 0.042

5.3 ± 0.055

10.061 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.568 ± 0.026

3.808 ± 0.032

4.378 ± 0.027

3.377 ± 0.027

5.796 ± 0.039

6.102 ± 0.036

4.939 ± 0.035

6.813 ± 0.037

1.234 ± 0.019

3.047 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski