Epibacterium multivorans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Epibacterium

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3869 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P1G7T0|A0A0P1G7T0_9RHOB Mutator family transposase OS=Epibacterium multivorans OX=928856 GN=TRM7557_01475 PE=3 SV=1
MM1 pKa = 7.56SYY3 pKa = 11.45VNTSKK8 pKa = 10.55IVLSTAAVLMLGTASLAQAEE28 pKa = 4.57TTVALVLPGSIADD41 pKa = 4.06GGWNAGAYY49 pKa = 9.45QGLQALKK56 pKa = 10.77ADD58 pKa = 4.02GFDD61 pKa = 3.08IAFSEE66 pKa = 4.45NVSQADD72 pKa = 3.18IPAVVQGYY80 pKa = 11.06ADD82 pKa = 4.04DD83 pKa = 6.01GYY85 pKa = 11.37DD86 pKa = 3.39LVIGHH91 pKa = 7.36GYY93 pKa = 9.93QFGSLFAEE101 pKa = 4.47ISEE104 pKa = 4.81EE105 pKa = 4.08YY106 pKa = 10.14PEE108 pKa = 4.02QAFFATTSAPGNTEE122 pKa = 3.47IPSNALYY129 pKa = 11.13VEE131 pKa = 4.52FRR133 pKa = 11.84YY134 pKa = 8.85TDD136 pKa = 3.01AAYY139 pKa = 10.39GMGALAALMSDD150 pKa = 3.73GKK152 pKa = 10.98AVGVVGGGDD161 pKa = 3.25NPTTQGMAKK170 pKa = 10.07AFVEE174 pKa = 4.22AAEE177 pKa = 4.14ATKK180 pKa = 10.46PGLKK184 pKa = 10.04GYY186 pKa = 10.66AIVTGDD192 pKa = 3.69YY193 pKa = 11.03NDD195 pKa = 3.7AAKK198 pKa = 10.57GRR200 pKa = 11.84EE201 pKa = 4.09AASTMIGNGADD212 pKa = 4.32VIWHH216 pKa = 6.0TADD219 pKa = 2.72ITGIGAIEE227 pKa = 4.36GASSQGAQVIGMFADD242 pKa = 3.67QTEE245 pKa = 4.46LAPSAMGTSLSANNAGLVQEE265 pKa = 4.39VAKK268 pKa = 10.03MVADD272 pKa = 4.77GSFEE276 pKa = 4.1GGGMWEE282 pKa = 4.08PALGFSWLPVYY293 pKa = 11.0GDD295 pKa = 3.46ASYY298 pKa = 8.73NTDD301 pKa = 5.24LISDD305 pKa = 4.16DD306 pKa = 3.24VWAQFLDD313 pKa = 3.08IWSKK317 pKa = 10.44VDD319 pKa = 3.17SGEE322 pKa = 4.3IEE324 pKa = 4.05PASS327 pKa = 3.44

Molecular weight: 33.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N7LZW9|A0A0N7LZW9_9RHOB Hint_2 domain-containing protein OS=Epibacterium multivorans OX=928856 GN=TRM7557_02064 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3869

0

3869

1235929

31

2766

319.4

34.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.08 ± 0.053

0.926 ± 0.014

6.132 ± 0.043

6.029 ± 0.036

3.85 ± 0.026

8.521 ± 0.048

2.061 ± 0.021

4.932 ± 0.028

3.431 ± 0.032

10.245 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.753 ± 0.017

2.759 ± 0.023

4.927 ± 0.031

3.545 ± 0.024

6.223 ± 0.043

5.325 ± 0.026

5.505 ± 0.033

7.183 ± 0.031

1.352 ± 0.017

2.221 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski