Planomicrobium glaciei
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3811 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G7Z4E4|A0A1G7Z4E4_9BACL Indole-3-glycerol phosphate synthase OS=Planomicrobium glaciei OX=459472 GN=trpC PE=3 SV=1
MM1 pKa = 7.53 EE2 pKa = 5.77 LFGFPMEE9 pKa = 4.2 QVYY12 pKa = 10.51 LYY14 pKa = 9.93 TLVAAGALTVLYY26 pKa = 10.91 VLFADD31 pKa = 4.63 IADD34 pKa = 4.55 AGDD37 pKa = 3.83 GLPFFNPAVILAFVTLLAAAGYY59 pKa = 9.1 VLEE62 pKa = 4.97 LATSLSDD69 pKa = 4.26 LAILGIAAVAAVVLDD84 pKa = 3.12 ILLYY88 pKa = 10.4 FFVLLPLSSAEE99 pKa = 3.83 SSIAYY104 pKa = 8.77 TEE106 pKa = 4.17 EE107 pKa = 4.05 SLLGQVAKK115 pKa = 10.65 VIVPIPVDD123 pKa = 3.78 GYY125 pKa = 9.99 GEE127 pKa = 4.39 VVIEE131 pKa = 4.61 TYY133 pKa = 11.15 GGMISKK139 pKa = 10.26 RR140 pKa = 11.84 AAGFDD145 pKa = 3.59 NEE147 pKa = 5.79 AIGQDD152 pKa = 3.32 RR153 pKa = 11.84 QVLIIQVNDD162 pKa = 3.23 GTLYY166 pKa = 10.41 VRR168 pKa = 11.84 DD169 pKa = 4.03 YY170 pKa = 11.18 EE171 pKa = 4.98 PIFSTEE177 pKa = 3.38 KK178 pKa = 10.63 RR179 pKa = 11.84 II180 pKa = 4.01
Molecular weight: 19.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.785
IPC2_protein 3.897
IPC_protein 3.834
Toseland 3.643
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.668
Grimsley 3.554
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.113
Thurlkill 3.694
EMBOSS 3.745
Sillero 3.948
Patrickios 0.604
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.852
Protein with the highest isoelectric point:
>tr|A0A1G8K176|A0A1G8K176_9BACL Uncharacterized protein OS=Planomicrobium glaciei OX=459472 GN=SAMN04487975_11596 PE=4 SV=1
MM1 pKa = 7.64 LYY3 pKa = 9.48 IHH5 pKa = 7.32 CSPSIWSKK13 pKa = 10.1 PAEE16 pKa = 4.19 KK17 pKa = 9.79 TLLLLQCCAIRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.37 DD31 pKa = 3.64 LASQASANVLPAGQRR46 pKa = 11.84 KK47 pKa = 9.0 RR48 pKa = 11.84 RR49 pKa = 11.84 DD50 pKa = 3.13 SRR52 pKa = 11.84 DD53 pKa = 3.14 PARR56 pKa = 11.84 RR57 pKa = 11.84 TAAEE61 pKa = 4.02 EE62 pKa = 3.51 AWRR65 pKa = 11.84 IARR68 pKa = 11.84 GKK70 pKa = 10.12 RR71 pKa = 11.84 SWFAEE76 pKa = 3.87 YY77 pKa = 10.54 RR78 pKa = 3.64
Molecular weight: 9.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.633
IPC_protein 10.526
Toseland 10.789
ProMoST 10.921
Dawson 10.862
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.023
Grimsley 10.906
Solomon 11.023
Lehninger 10.994
Nozaki 10.789
DTASelect 10.599
Thurlkill 10.789
EMBOSS 11.199
Sillero 10.804
Patrickios 10.789
IPC_peptide 11.038
IPC2_peptide 9.867
IPC2.peptide.svr19 8.782
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3811
0
3811
1129360
39
1605
296.3
33.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.425 ± 0.044
0.6 ± 0.01
5.094 ± 0.029
7.821 ± 0.047
4.613 ± 0.031
7.272 ± 0.04
2.038 ± 0.021
7.064 ± 0.04
6.124 ± 0.036
9.855 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.873 ± 0.02
3.835 ± 0.026
3.832 ± 0.021
3.694 ± 0.027
4.193 ± 0.029
5.825 ± 0.029
5.318 ± 0.023
7.147 ± 0.031
1.057 ± 0.014
3.318 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here