Halovirus HRTV-4
Average proteome isoelectric point is 5.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4TLV2|R4TLV2_9VIRU Uncharacterized protein OS=Halovirus HRTV-4 OX=1273752 GN=46 PE=4 SV=1
MM1 pKa = 7.26 SQTKK5 pKa = 8.1 TVQTAYY11 pKa = 10.65 GDD13 pKa = 3.43 VDD15 pKa = 4.16 VEE17 pKa = 4.46 VVEE20 pKa = 5.53 CDD22 pKa = 3.1 SCGNTIGKK30 pKa = 9.84 DD31 pKa = 3.28 EE32 pKa = 4.26 ARR34 pKa = 11.84 EE35 pKa = 3.77 FTLGNRR41 pKa = 11.84 EE42 pKa = 3.53 GHH44 pKa = 5.95 ACEE47 pKa = 4.29 HH48 pKa = 6.65 CVGEE52 pKa = 4.7 GPISFPSKK60 pKa = 10.45 YY61 pKa = 10.46 GSFDD65 pKa = 3.1 MGEE68 pKa = 4.13 SSDD71 pKa = 4.0 GRR73 pKa = 11.84 FLNFLVWAPVVIPLLMMFGPFEE95 pKa = 4.77 NDD97 pKa = 3.39 DD98 pKa = 3.71 NDD100 pKa = 3.99 LMRR103 pKa = 11.84 GAMWVAWTITIYY115 pKa = 11.22 ALVIGYY121 pKa = 8.63 VALVALL127 pKa = 4.75
Molecular weight: 13.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.93
IPC2_protein 4.228
IPC_protein 4.139
Toseland 3.961
ProMoST 4.279
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.012
Rodwell 3.973
Grimsley 3.872
Solomon 4.101
Lehninger 4.05
Nozaki 4.228
DTASelect 4.406
Thurlkill 3.999
EMBOSS 4.024
Sillero 4.253
Patrickios 1.964
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.147
Protein with the highest isoelectric point:
>tr|R4TLW4|R4TLW4_9VIRU Uncharacterized protein OS=Halovirus HRTV-4 OX=1273752 GN=61 PE=4 SV=1
MM1 pKa = 7.25 VFRR4 pKa = 11.84 RR5 pKa = 11.84 PLSHH9 pKa = 6.59 GRR11 pKa = 11.84 PATQTTNTSGVVASRR26 pKa = 11.84 MHH28 pKa = 6.55 PADD31 pKa = 4.61 AIMWLAVVTVVMCGVVGVALLVV53 pKa = 3.6
Molecular weight: 5.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.135
IPC2_protein 9.75
IPC_protein 11.359
Toseland 11.52
ProMoST 12.018
Dawson 11.52
Bjellqvist 11.52
Wikipedia 12.003
Rodwell 11.023
Grimsley 11.564
Solomon 12.032
Lehninger 11.93
Nozaki 11.535
DTASelect 11.52
Thurlkill 11.52
EMBOSS 12.018
Sillero 11.52
Patrickios 11.023
IPC_peptide 12.032
IPC2_peptide 11.038
IPC2.peptide.svr19 9.802
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
73
0
73
11341
32
1090
155.4
17.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.726 ± 0.286
1.076 ± 0.183
9.876 ± 0.435
8.156 ± 0.414
2.778 ± 0.163
7.945 ± 0.307
2.143 ± 0.266
3.721 ± 0.166
3.121 ± 0.31
6.745 ± 0.296
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.204 ± 0.261
3.553 ± 0.268
4.294 ± 0.218
3.095 ± 0.162
6.243 ± 0.313
5.767 ± 0.254
8.006 ± 0.557
7.283 ± 0.311
1.49 ± 0.114
2.778 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here