Promicromonospora thailandica
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5167 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A397LR42|A0A397LR42_9MICO 8-oxo-dGTP pyrophosphatase MutT (NUDIX family) OS=Promicromonospora thailandica OX=765201 GN=C7338_3333 PE=4 SV=1
MM1 pKa = 7.48 RR2 pKa = 11.84 QQLSRR7 pKa = 11.84 LLAGLAVSALVALPLASTGAVAAEE31 pKa = 3.95 PAEE34 pKa = 4.37 EE35 pKa = 5.57 GVTAWSPPAKK45 pKa = 10.0 SAPKK49 pKa = 10.17 DD50 pKa = 3.58 RR51 pKa = 11.84 APARR55 pKa = 11.84 ADD57 pKa = 3.39 GATTAAAEE65 pKa = 4.32 VADD68 pKa = 5.49 QVLTWTADD76 pKa = 3.4 GGVSEE81 pKa = 4.82 YY82 pKa = 11.39 ASAPATATAGATTIVFEE99 pKa = 4.26 NSRR102 pKa = 11.84 ATGNNIAMSHH112 pKa = 5.77 TLTFDD117 pKa = 3.08 TSTPGYY123 pKa = 9.43 NHH125 pKa = 7.54 DD126 pKa = 3.71 VTLDD130 pKa = 3.35 ILANPLDD137 pKa = 4.49 ANGGRR142 pKa = 11.84 YY143 pKa = 7.6 EE144 pKa = 4.31 AEE146 pKa = 4.1 VVLTPGTYY154 pKa = 9.31 RR155 pKa = 11.84 YY156 pKa = 9.34 FCAIPGHH163 pKa = 4.92 STMVGEE169 pKa = 4.47 LVVTEE174 pKa = 4.69 GPGEE178 pKa = 4.2 DD179 pKa = 3.06 TTAPVVTPQVAGQQDD194 pKa = 3.57 GTGAYY199 pKa = 9.17 IGTATVSLAAEE210 pKa = 4.38 DD211 pKa = 5.25 DD212 pKa = 3.65 ISGVASVEE220 pKa = 4.0 YY221 pKa = 10.55 ALDD224 pKa = 3.83 GGEE227 pKa = 3.88 FAPYY231 pKa = 10.23 GEE233 pKa = 4.39 PVVLDD238 pKa = 3.73 EE239 pKa = 5.17 PGTYY243 pKa = 10.43 SLAYY247 pKa = 9.98 RR248 pKa = 11.84 ATDD251 pKa = 3.16 NAGNVSEE258 pKa = 5.23 PGTLDD263 pKa = 3.34 VTVVSADD270 pKa = 3.93 GEE272 pKa = 4.52 DD273 pKa = 3.52 TTAPTVTAEE282 pKa = 4.19 VTGEE286 pKa = 4.0 LDD288 pKa = 4.18 DD289 pKa = 5.33 DD290 pKa = 4.2 GAYY293 pKa = 10.69 VGTATVNLAALDD305 pKa = 4.15 AGSGVASVEE314 pKa = 3.8 YY315 pKa = 10.71 DD316 pKa = 3.3 LDD318 pKa = 3.98 GAGYY322 pKa = 10.01 AAYY325 pKa = 8.39 TDD327 pKa = 4.24 PVAVDD332 pKa = 3.43 TPGEE336 pKa = 4.07 HH337 pKa = 5.37 TVTYY341 pKa = 10.37 RR342 pKa = 11.84 ATDD345 pKa = 3.24 NAGNVSEE352 pKa = 4.97 PGSATFTVVEE362 pKa = 4.71 PSQEE366 pKa = 4.08 DD367 pKa = 3.84 TTPPTVTAHH376 pKa = 6.51 VMGEE380 pKa = 3.9 QDD382 pKa = 2.94 GDD384 pKa = 3.65 GAYY387 pKa = 10.32 VGSATVHH394 pKa = 6.28 LVAEE398 pKa = 4.79 DD399 pKa = 3.59 EE400 pKa = 4.4 ASEE403 pKa = 4.41 VTSVEE408 pKa = 3.78 YY409 pKa = 10.97 DD410 pKa = 3.16 LDD412 pKa = 4.05 GAGWTDD418 pKa = 3.23 YY419 pKa = 11.28 GDD421 pKa = 3.83 PVLVDD426 pKa = 3.03 VTGSHH431 pKa = 6.04 TLTYY435 pKa = 10.35 RR436 pKa = 11.84 ATDD439 pKa = 3.28 AAGNTSEE446 pKa = 4.59 PGSVEE451 pKa = 3.66 IVVVGGADD459 pKa = 3.71 PPDD462 pKa = 3.93 TTPPIASAQVDD473 pKa = 4.09 GDD475 pKa = 3.48 QDD477 pKa = 3.85 DD478 pKa = 4.06 YY479 pKa = 12.07 GVYY482 pKa = 10.71 LEE484 pKa = 4.52 VATVSISARR493 pKa = 11.84 DD494 pKa = 3.91 DD495 pKa = 3.52 EE496 pKa = 5.08 SGVAFVEE503 pKa = 4.35 WALGDD508 pKa = 3.66 TAFRR512 pKa = 11.84 EE513 pKa = 4.27 YY514 pKa = 10.4 TGPFDD519 pKa = 3.65 VTDD522 pKa = 4.84 PGAHH526 pKa = 6.68 ALTYY530 pKa = 10.38 RR531 pKa = 11.84 ATDD534 pKa = 3.13 NAGNVTRR541 pKa = 11.84 TGTLRR546 pKa = 11.84 FSVSRR551 pKa = 11.84 STIDD555 pKa = 3.01 ACPVSDD561 pKa = 4.4 EE562 pKa = 4.48 RR563 pKa = 11.84 PTVILGNIDD572 pKa = 3.84 SKK574 pKa = 9.75 VTNYY578 pKa = 10.95 DD579 pKa = 3.24 SGNGCTVADD588 pKa = 5.69 LIDD591 pKa = 5.16 ADD593 pKa = 4.42 GAWVSHH599 pKa = 5.66 NAFVLHH605 pKa = 6.02 VNQVADD611 pKa = 4.27 RR612 pKa = 11.84 LDD614 pKa = 3.92 DD615 pKa = 4.55 ADD617 pKa = 4.59 VITATEE623 pKa = 3.9 ATRR626 pKa = 11.84 LIRR629 pKa = 11.84 AARR632 pKa = 11.84 QSDD635 pKa = 3.71 VGRR638 pKa = 4.31
Molecular weight: 65.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.757
IPC_protein 3.795
Toseland 3.567
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.465
Solomon 3.783
Lehninger 3.732
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.91
Patrickios 1.481
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.814
Protein with the highest isoelectric point:
>tr|A0A397LHD0|A0A397LHD0_9MICO Uncharacterized protein OS=Promicromonospora thailandica OX=765201 GN=C7338_0228 PE=4 SV=1
MM1 pKa = 6.92 GTGAAGTTALAAEE14 pKa = 4.45 RR15 pKa = 11.84 DD16 pKa = 3.97 GTTTTVRR23 pKa = 11.84 AGPSTGTTVRR33 pKa = 11.84 AGTTGPAGTTGLVATTGPAGRR54 pKa = 11.84 VAGVSGRR61 pKa = 11.84 SGVVTTAGVRR71 pKa = 11.84 TVRR74 pKa = 11.84 AVRR77 pKa = 11.84 TASGATAARR86 pKa = 11.84 VTEE89 pKa = 4.05 ATAARR94 pKa = 11.84 GATGRR99 pKa = 11.84 SGGTTATGRR108 pKa = 11.84 AGRR111 pKa = 11.84 TRR113 pKa = 11.84 TGTAAVRR120 pKa = 11.84 GVRR123 pKa = 11.84 RR124 pKa = 11.84 GTTTTGRR131 pKa = 11.84 VGRR134 pKa = 11.84 SVTGMTGRR142 pKa = 11.84 VGRR145 pKa = 11.84 SRR147 pKa = 11.84 SVAGTVRR154 pKa = 11.84 GVTTGWVTGRR164 pKa = 11.84 SVVVTTRR171 pKa = 11.84 PGARR175 pKa = 11.84 TVTAAIVRR183 pKa = 11.84 GGPSTVMTGRR193 pKa = 11.84 AGPSRR198 pKa = 11.84 TVAGIGRR205 pKa = 11.84 GVTTGWVTGRR215 pKa = 11.84 SGGAVTTVPAVRR227 pKa = 11.84 SVTATTGRR235 pKa = 11.84 AGRR238 pKa = 11.84 SRR240 pKa = 11.84 TAVGIGRR247 pKa = 11.84 AATTAGARR255 pKa = 11.84 SGGVVRR261 pKa = 11.84 TGRR264 pKa = 11.84 GVRR267 pKa = 11.84 STTVATGRR275 pKa = 11.84 AARR278 pKa = 11.84 TGRR281 pKa = 11.84 TAPGVPSVIGVTVRR295 pKa = 11.84 AGRR298 pKa = 11.84 SRR300 pKa = 11.84 SVAGIGRR307 pKa = 11.84 GVMTGWVTGRR317 pKa = 11.84 SVVVATRR324 pKa = 11.84 RR325 pKa = 11.84 GVRR328 pKa = 11.84 TVTAAIVRR336 pKa = 11.84 GGPSTVMTGRR346 pKa = 11.84 AGRR349 pKa = 11.84 SRR351 pKa = 11.84 SVAGIGRR358 pKa = 11.84 GVMTAGARR366 pKa = 11.84 SEE368 pKa = 4.47 GAVTTVPAVRR378 pKa = 11.84 SRR380 pKa = 11.84 TVATGCAATTVGFRR394 pKa = 11.84 SGGTTAVATGASSAGATVLPATATSVGGTSVAGTSGRR431 pKa = 11.84 RR432 pKa = 11.84 ASRR435 pKa = 11.84 NRR437 pKa = 11.84 RR438 pKa = 11.84 SPRR441 pKa = 11.84 TPRR444 pKa = 11.84 SVSSTGRR451 pKa = 11.84 HH452 pKa = 5.18 GLACGAA458 pKa = 4.4
Molecular weight: 44.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.509
IPC2_protein 11.169
IPC_protein 12.778
Toseland 12.939
ProMoST 13.437
Dawson 12.939
Bjellqvist 12.939
Wikipedia 13.422
Rodwell 12.442
Grimsley 12.983
Solomon 13.437
Lehninger 13.334
Nozaki 12.939
DTASelect 12.939
Thurlkill 12.939
EMBOSS 13.437
Sillero 12.939
Patrickios 12.149
IPC_peptide 13.437
IPC2_peptide 12.427
IPC2.peptide.svr19 9.196
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5167
0
5167
1812030
30
2269
350.7
37.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.331 ± 0.052
0.519 ± 0.008
6.461 ± 0.031
5.475 ± 0.028
2.631 ± 0.018
9.799 ± 0.034
2.043 ± 0.016
2.932 ± 0.021
1.448 ± 0.021
10.066 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.585 ± 0.013
1.623 ± 0.017
6.135 ± 0.027
2.619 ± 0.018
7.671 ± 0.04
4.93 ± 0.022
6.473 ± 0.032
9.69 ± 0.037
1.609 ± 0.017
1.959 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here