Parashewanella spongiae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Parashewanella

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3857 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A6TCS2|A0A3A6TCS2_9GAMM Methylcrotonoyl-CoA carboxylase OS=Parashewanella spongiae OX=342950 GN=D5R81_14950 PE=4 SV=1
MM1 pKa = 7.2PTRR4 pKa = 11.84TRR6 pKa = 11.84KK7 pKa = 9.89LLIAIAVSSIFSASVVAGDD26 pKa = 4.27HH27 pKa = 6.28NEE29 pKa = 3.51ITIDD33 pKa = 3.37QRR35 pKa = 11.84TVSNEE40 pKa = 3.52GQTATSTQFGSFNQSTINQEE60 pKa = 3.29GDD62 pKa = 3.46AQDD65 pKa = 3.37SSVIQNGEE73 pKa = 4.29YY74 pKa = 10.22NVSSLTSNGYY84 pKa = 11.11GNTQFIEE91 pKa = 4.01QTGEE95 pKa = 4.07YY96 pKa = 9.75NNSTVAVYY104 pKa = 11.01GDD106 pKa = 3.88ANVNDD111 pKa = 3.79VTQDD115 pKa = 2.85ISNYY119 pKa = 9.83SYY121 pKa = 11.5SEE123 pKa = 3.89TRR125 pKa = 11.84GSDD128 pKa = 3.34NEE130 pKa = 4.02NTITQNGISNYY141 pKa = 10.28NYY143 pKa = 10.25FSFSGDD149 pKa = 3.64NNVHH153 pKa = 6.1TLDD156 pKa = 4.37QAGEE160 pKa = 4.26DD161 pKa = 3.78NQSYY165 pKa = 9.82FYY167 pKa = 11.22SFNEE171 pKa = 3.93ANRR174 pKa = 11.84NTATITQGGQYY185 pKa = 9.05NQSRR189 pKa = 11.84IVNVGSDD196 pKa = 3.63DD197 pKa = 3.94NEE199 pKa = 4.09FVISQDD205 pKa = 2.96GDD207 pKa = 4.16SNSSLIQGTVGHH219 pKa = 6.39TNSFNISQVDD229 pKa = 3.86FLNTARR235 pKa = 11.84LINVAGNNNEE245 pKa = 3.75ITVDD249 pKa = 3.79QDD251 pKa = 2.94GDD253 pKa = 3.76QNNVGDD259 pKa = 3.98GDD261 pKa = 3.89VAEE264 pKa = 4.88FSGDD268 pKa = 3.48NNVVNITQTGASNRR282 pKa = 11.84TDD284 pKa = 3.09MRR286 pKa = 11.84VLGDD290 pKa = 3.67ANTVSLHH297 pKa = 5.66QDD299 pKa = 3.22GNSHH303 pKa = 5.68YY304 pKa = 11.57ARR306 pKa = 11.84MTTDD310 pKa = 3.17GNSNTVNASQSGDD323 pKa = 2.84INMIDD328 pKa = 3.35ILTTGDD334 pKa = 3.48EE335 pKa = 4.21NSIEE339 pKa = 4.02LAQVGGSNDD348 pKa = 2.64ISGIDD353 pKa = 3.69GVSADD358 pKa = 4.17FGVTGSNNTLTISQTGNLNSVFGSSTGSANSISVAQTGTSNTAVTTQNN406 pKa = 3.14

Molecular weight: 42.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A6TUA9|A0A3A6TUA9_9GAMM DEDD_Tnp_IS110 domain-containing protein OS=Parashewanella spongiae OX=342950 GN=D5R81_12240 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.14RR13 pKa = 11.84KK14 pKa = 9.13RR15 pKa = 11.84SHH17 pKa = 6.17GFRR20 pKa = 11.84ARR22 pKa = 11.84MATVGGRR29 pKa = 11.84KK30 pKa = 9.01VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.39GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3857

0

3857

1325279

33

2457

343.6

38.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.688 ± 0.043

1.249 ± 0.02

5.452 ± 0.031

6.19 ± 0.041

4.232 ± 0.024

6.115 ± 0.042

2.316 ± 0.017

6.752 ± 0.028

6.304 ± 0.04

10.372 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.017

4.916 ± 0.03

3.753 ± 0.025

4.726 ± 0.03

4.186 ± 0.03

7.335 ± 0.041

5.531 ± 0.029

6.389 ± 0.034

1.122 ± 0.015

3.032 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski