Capnocytophaga sp. oral taxon 338 str. F0234
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2444 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F0IDB7|F0IDB7_9FLAO Uncharacterized protein OS=Capnocytophaga sp. oral taxon 338 str. F0234 OX=888059 GN=HMPREF9071_0507 PE=4 SV=1
MM1 pKa = 7.73 SLEE4 pKa = 4.48 SIITHH9 pKa = 5.62 YY10 pKa = 10.6 RR11 pKa = 11.84 NCLAALPDD19 pKa = 3.84 VILIGEE25 pKa = 4.19 IPEE28 pKa = 4.39 GAEE31 pKa = 4.35 SIPQEE36 pKa = 4.03 VSDD39 pKa = 4.22 WGVPAYY45 pKa = 10.58 SAFLRR50 pKa = 11.84 LCNGGSFGDD59 pKa = 3.72 IILWSTEE66 pKa = 3.72 EE67 pKa = 4.56 LLDD70 pKa = 3.53 NQYY73 pKa = 10.57 RR74 pKa = 11.84 IPSDD78 pKa = 3.4 QPSWCEE84 pKa = 3.46 IGQLLYY90 pKa = 11.02 APLFLDD96 pKa = 3.54 KK97 pKa = 10.0 HH98 pKa = 4.89 TQHH101 pKa = 7.29 LIFPADD107 pKa = 3.3 SYY109 pKa = 11.82 EE110 pKa = 4.43 GIEE113 pKa = 4.44 EE114 pKa = 4.13 IDD116 pKa = 3.26 VDD118 pKa = 4.15 FDD120 pKa = 4.16 TFVKK124 pKa = 10.47 EE125 pKa = 4.11 YY126 pKa = 10.59 IFGSKK131 pKa = 10.18 YY132 pKa = 8.85 KK133 pKa = 10.3 EE134 pKa = 3.85 KK135 pKa = 10.5 IIGYY139 pKa = 9.95 DD140 pKa = 3.35 EE141 pKa = 6.35 ANDD144 pKa = 3.71 DD145 pKa = 3.89 WSDD148 pKa = 3.59 FLNN151 pKa = 3.87
Molecular weight: 17.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.797
IPC2_protein 3.973
IPC_protein 3.935
Toseland 3.745
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.821
Rodwell 3.77
Grimsley 3.656
Solomon 3.897
Lehninger 3.859
Nozaki 4.024
DTASelect 4.215
Thurlkill 3.783
EMBOSS 3.834
Sillero 4.05
Patrickios 0.769
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.933
Protein with the highest isoelectric point:
>tr|F0IH38|F0IH38_9FLAO Uncharacterized protein OS=Capnocytophaga sp. oral taxon 338 str. F0234 OX=888059 GN=HMPREF9071_1885 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.13 RR10 pKa = 11.84 KK11 pKa = 9.48 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.49 HH16 pKa = 3.94 GFRR19 pKa = 11.84 EE20 pKa = 4.19 RR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.93 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.08 GRR39 pKa = 11.84 KK40 pKa = 8.3 KK41 pKa = 9.83 LTVSSEE47 pKa = 3.9 PRR49 pKa = 11.84 HH50 pKa = 5.77 KK51 pKa = 10.61 KK52 pKa = 9.84
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2444
0
2444
750425
38
3423
307.0
34.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.143 ± 0.048
0.863 ± 0.017
4.92 ± 0.03
7.001 ± 0.058
5.182 ± 0.045
6.05 ± 0.054
1.912 ± 0.021
8.007 ± 0.057
8.045 ± 0.055
9.538 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.153 ± 0.02
5.29 ± 0.045
3.622 ± 0.036
3.947 ± 0.035
3.922 ± 0.029
6.055 ± 0.032
5.823 ± 0.058
5.808 ± 0.048
1.048 ± 0.017
4.672 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here