Gordonia amarae NBRC 15530

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Gordoniaceae; Gordonia; Gordonia amarae

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4691 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G7GNG8|G7GNG8_9ACTN DapB_N domain-containing protein OS=Gordonia amarae NBRC 15530 OX=1075090 GN=GOAMR_28_00300 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84ISHH5 pKa = 6.84KK6 pKa = 10.71NRR8 pKa = 11.84ARR10 pKa = 11.84LIAAAAGVSLFLNPGQAAADD30 pKa = 4.27DD31 pKa = 4.23DD32 pKa = 4.51TGSGTGSGASSHH44 pKa = 6.67SDD46 pKa = 2.75SSGGTNTSGGNSASGDD62 pKa = 3.92SQSTSSPGDD71 pKa = 3.54SNPAGNSGTTGGAGGSDD88 pKa = 3.48VTGGPDD94 pKa = 3.26STGGTDD100 pKa = 3.38NTASDD105 pKa = 4.65AGPSDD110 pKa = 3.94DD111 pKa = 4.26TDD113 pKa = 3.8SGSGSSPDD121 pKa = 3.39STGTSGNTTPADD133 pKa = 3.67TDD135 pKa = 3.76TTPSDD140 pKa = 3.6TTPADD145 pKa = 3.49AVPSGNEE152 pKa = 3.74TAGTTRR158 pKa = 11.84PPTEE162 pKa = 4.14TDD164 pKa = 2.88HH165 pKa = 6.85TEE167 pKa = 4.12PPAPADD173 pKa = 3.6TTVTPDD179 pKa = 3.29TTNTWTEE186 pKa = 3.87PTNTPTAPTTTGDD199 pKa = 3.67TTPTTSTAEE208 pKa = 4.15STSPDD213 pKa = 3.17ATGTADD219 pKa = 3.72TDD221 pKa = 3.74TTPPIGDD228 pKa = 3.9AEE230 pKa = 4.58TPGDD234 pKa = 3.5NSGPSEE240 pKa = 4.52PDD242 pKa = 2.96PTTLRR247 pKa = 11.84PLGFTAPAFAEE258 pKa = 4.4EE259 pKa = 4.82PEE261 pKa = 4.6TTGEE265 pKa = 4.24PEE267 pKa = 4.16TFSNAVFFAPPPATTPPAAPAPATPLTTLLTFFGIPPVGGPTAPTALTTPAPWTLLWWIQRR328 pKa = 11.84TQSLLNNQTPTATSVLDD345 pKa = 3.98PVTDD349 pKa = 4.76GYY351 pKa = 9.74TISLTTDD358 pKa = 3.55DD359 pKa = 5.28ADD361 pKa = 4.06GDD363 pKa = 4.28PVATTVYY370 pKa = 9.81HH371 pKa = 5.81QPTHH375 pKa = 5.07GTVVKK380 pKa = 10.78NSDD383 pKa = 3.18GAFTYY388 pKa = 10.38TRR390 pKa = 11.84DD391 pKa = 3.94QNSSAADD398 pKa = 3.2QFTIKK403 pKa = 10.62VSDD406 pKa = 3.95NGPHH410 pKa = 5.66LHH412 pKa = 6.76GLATVIGLFTGHH424 pKa = 7.39DD425 pKa = 3.51PHH427 pKa = 6.16STYY430 pKa = 9.79VTVNIPEE437 pKa = 4.29GTAAPANSAPVIDD450 pKa = 5.78AIDD453 pKa = 3.89YY454 pKa = 10.51SPQGSDD460 pKa = 3.33GVITGTTTATDD471 pKa = 3.52HH472 pKa = 7.59DD473 pKa = 5.34GDD475 pKa = 4.41TVTYY479 pKa = 9.19TYY481 pKa = 10.23TWDD484 pKa = 3.57TTKK487 pKa = 11.1GAIDD491 pKa = 4.18YY492 pKa = 7.47DD493 pKa = 3.96TGTGAFTFTPTYY505 pKa = 7.62TARR508 pKa = 11.84HH509 pKa = 5.59AAASDD514 pKa = 3.22NATPGDD520 pKa = 3.56KK521 pKa = 10.93ALTVTVNASDD531 pKa = 3.76GTLSDD536 pKa = 4.04TEE538 pKa = 4.61NFTLDD543 pKa = 2.87ILAANEE549 pKa = 4.32SVASNAPTSPLGTIDD564 pKa = 4.68RR565 pKa = 11.84DD566 pKa = 3.74MNAATYY572 pKa = 10.96GNTTGSLSGIATDD585 pKa = 5.06ADD587 pKa = 4.23NDD589 pKa = 3.99TLAYY593 pKa = 10.47SVTTQGANGTVTIVGDD609 pKa = 3.97SFTYY613 pKa = 10.36TPDD616 pKa = 3.18SANRR620 pKa = 11.84AFTDD624 pKa = 3.56TFTVTVDD631 pKa = 3.8DD632 pKa = 4.35GHH634 pKa = 7.14GSTTDD639 pKa = 3.07VTITVDD645 pKa = 3.74TVPMYY650 pKa = 10.28YY651 pKa = 10.19HH652 pKa = 7.25HH653 pKa = 7.79IDD655 pKa = 3.42PTNQFWDD662 pKa = 3.9PSDD665 pKa = 4.23AGFLTTIPAIVNPTGVVDD683 pKa = 3.78YY684 pKa = 11.04HH685 pKa = 8.31VDD687 pKa = 3.19EE688 pKa = 4.8TGNIIVNNTGSDD700 pKa = 4.25PIMLWSITNDD710 pKa = 2.64EE711 pKa = 4.21VTALNAGGPATFEE724 pKa = 4.1ALVIASGSSATIPPRR739 pKa = 11.84AGSTPFIWDD748 pKa = 3.61QIILLQAPRR757 pKa = 11.84GAEE760 pKa = 4.29PILLGQIVLNDD771 pKa = 3.7GTILSDD777 pKa = 4.31DD778 pKa = 5.72GIPQTPVEE786 pKa = 4.41VTVTPGSNGAVNGVVNGHH804 pKa = 5.45QAGTTYY810 pKa = 10.49SVQGGTPTGGGYY822 pKa = 7.59LTPDD826 pKa = 3.23GGTVVFDD833 pKa = 3.73NSSGTFVFTPEE844 pKa = 5.57GEE846 pKa = 4.13DD847 pKa = 3.5QYY849 pKa = 11.15WAGGGGPTTQAFVILADD866 pKa = 4.63DD867 pKa = 4.34GVNPPRR873 pKa = 11.84AIAVTVPIDD882 pKa = 4.46PIDD885 pKa = 3.79LVVIDD890 pKa = 4.05MTGLNSTDD898 pKa = 3.23SPRR901 pKa = 11.84YY902 pKa = 9.21SYY904 pKa = 11.19GVSDD908 pKa = 3.71TSGTPILTVVDD919 pKa = 3.59RR920 pKa = 11.84TTAQSHH926 pKa = 6.9AIALPSGHH934 pKa = 7.4DD935 pKa = 3.4NTSTVTFSADD945 pKa = 2.43GGYY948 pKa = 10.8AHH950 pKa = 7.2ITNSGGSTVSVIDD963 pKa = 3.55LTTNQVIGTYY973 pKa = 9.88IVTDD977 pKa = 3.48NGDD980 pKa = 3.74GTKK983 pKa = 10.5SLAWTGGTPSGFVMASFVDD1002 pKa = 3.51NGPITGADD1010 pKa = 3.52FSIGAEE1016 pKa = 3.99GDD1018 pKa = 3.28QAIIVATQTDD1028 pKa = 3.86GTYY1031 pKa = 9.61TVYY1034 pKa = 10.8LADD1037 pKa = 3.75SRR1039 pKa = 11.84TGDD1042 pKa = 3.28RR1043 pKa = 11.84TALSTANPQNGTGTPTVTFIGSTGIALLADD1073 pKa = 4.03GTTVTSISVTDD1084 pKa = 3.79FAVLQQTVVGGDD1096 pKa = 3.5LAFDD1100 pKa = 3.43WVVNPNSQFAYY1111 pKa = 10.15GVTADD1116 pKa = 3.71PTSPVTSVTVVEE1128 pKa = 4.66LTTGTITEE1136 pKa = 4.22IAVPPTSAYY1145 pKa = 10.39AIDD1148 pKa = 4.17ANGSALYY1155 pKa = 9.45TYY1157 pKa = 10.93VLDD1160 pKa = 4.33PFTFSTQVTRR1170 pKa = 11.84TPFGDD1175 pKa = 3.3AGNAISVTVPGAVAGNVPEE1194 pKa = 4.77LSWSSFDD1201 pKa = 4.84DD1202 pKa = 3.55AGNYY1206 pKa = 9.93YY1207 pKa = 9.25LTTVDD1212 pKa = 3.47VSVVAGVPTLGAVKK1226 pKa = 10.08VHH1228 pKa = 6.31VFDD1231 pKa = 4.24QAMNEE1236 pKa = 3.73IDD1238 pKa = 4.09TIVTDD1243 pKa = 3.86VPSGQVAAMTSVADD1257 pKa = 3.6GTVYY1261 pKa = 11.13LMVADD1266 pKa = 5.01PGSAQNTYY1274 pKa = 9.45TIHH1277 pKa = 6.86RR1278 pKa = 11.84VSADD1282 pKa = 3.07GSVDD1286 pKa = 3.64DD1287 pKa = 5.35LADD1290 pKa = 3.68GVGWVTADD1298 pKa = 3.43QQHH1301 pKa = 5.7MFVLRR1306 pKa = 11.84TNDD1309 pKa = 2.85GSGYY1313 pKa = 8.6NTFSVLDD1320 pKa = 4.15LTTDD1324 pKa = 3.5TQSAPISVSTSYY1336 pKa = 11.14DD1337 pKa = 3.13GEE1339 pKa = 3.96FRR1341 pKa = 11.84VFEE1344 pKa = 4.26GKK1346 pKa = 10.21YY1347 pKa = 10.18LAIAYY1352 pKa = 7.87HH1353 pKa = 6.56AGSNVYY1359 pKa = 8.75VHH1361 pKa = 6.89ILDD1364 pKa = 3.58VDD1366 pKa = 4.01GNATDD1371 pKa = 4.09FVIGADD1377 pKa = 3.65DD1378 pKa = 4.26FEE1380 pKa = 4.74GLIYY1384 pKa = 10.57LDD1386 pKa = 4.49DD1387 pKa = 3.95QFSVITSGGGYY1398 pKa = 7.32TQATLIRR1405 pKa = 11.84AA1406 pKa = 3.85

Molecular weight: 143.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G7GQD9|G7GQD9_9ACTN 50S ribosomal protein L15 OS=Gordonia amarae NBRC 15530 OX=1075090 GN=rplO PE=3 SV=1
MM1 pKa = 7.46TKK3 pKa = 10.27GKK5 pKa = 8.73RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84SRR17 pKa = 11.84VHH19 pKa = 6.33GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVNSRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.8GRR42 pKa = 11.84AKK44 pKa = 9.67LTAA47 pKa = 4.21

Molecular weight: 5.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4691

0

4691

1599335

34

8820

340.9

36.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.748 ± 0.049

0.75 ± 0.011

6.7 ± 0.032

5.204 ± 0.029

2.968 ± 0.023

9.099 ± 0.03

2.097 ± 0.016

4.589 ± 0.02

2.417 ± 0.03

9.469 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.958 ± 0.016

2.153 ± 0.017

5.659 ± 0.024

2.739 ± 0.018

7.111 ± 0.036

5.644 ± 0.023

6.465 ± 0.028

8.721 ± 0.034

1.408 ± 0.013

2.1 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski