Porcine circovirus 1 (PCV1)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Circovirus

Average proteome isoelectric point is 7.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q80QL5|CAPSD_PCV1 Capsid protein OS=Porcine circovirus 1 OX=133704 GN=Cap PE=3 SV=2
MM1 pKa = 7.76PSKK4 pKa = 10.7KK5 pKa = 10.15SGPQPHH11 pKa = 6.44KK12 pKa = 10.3RR13 pKa = 11.84WVFTLNNPSEE23 pKa = 4.15EE24 pKa = 4.23EE25 pKa = 3.89KK26 pKa = 11.29NKK28 pKa = 9.72IRR30 pKa = 11.84EE31 pKa = 4.19LPISLFDD38 pKa = 3.63YY39 pKa = 8.0FVCGEE44 pKa = 4.09EE45 pKa = 4.15GLEE48 pKa = 4.14EE49 pKa = 4.45GRR51 pKa = 11.84TPHH54 pKa = 6.17LQGFANFAKK63 pKa = 10.18KK64 pKa = 7.57QTFNKK69 pKa = 8.67VKK71 pKa = 8.5WYY73 pKa = 10.26FGARR77 pKa = 11.84CHH79 pKa = 6.85IEE81 pKa = 3.87KK82 pKa = 10.86AKK84 pKa = 9.75GTDD87 pKa = 3.47QQNKK91 pKa = 8.81EE92 pKa = 4.08YY93 pKa = 10.19CSKK96 pKa = 10.39EE97 pKa = 3.48GHH99 pKa = 6.47ILIEE103 pKa = 4.56CGAPRR108 pKa = 11.84NQGKK112 pKa = 10.22RR113 pKa = 11.84SDD115 pKa = 3.69LSTAVSTLLEE125 pKa = 4.4TGSLVTVAEE134 pKa = 4.41QFPVTYY140 pKa = 10.26VRR142 pKa = 11.84NFRR145 pKa = 11.84GLAEE149 pKa = 4.1LLKK152 pKa = 11.05VSGKK156 pKa = 7.49MQQRR160 pKa = 11.84DD161 pKa = 3.45WKK163 pKa = 8.27TAVHH167 pKa = 6.3VIVGPPGCGKK177 pKa = 9.37SQWARR182 pKa = 11.84NFAEE186 pKa = 5.03PSDD189 pKa = 4.45TYY191 pKa = 10.64WKK193 pKa = 10.05PSRR196 pKa = 11.84NKK198 pKa = 8.96WWDD201 pKa = 3.68GYY203 pKa = 10.6HH204 pKa = 6.06GEE206 pKa = 4.32EE207 pKa = 5.11VVVLDD212 pKa = 5.98DD213 pKa = 4.52FYY215 pKa = 11.98GWLPWDD221 pKa = 4.33DD222 pKa = 4.85LLRR225 pKa = 11.84LCDD228 pKa = 4.28RR229 pKa = 11.84YY230 pKa = 10.51PLTVEE235 pKa = 4.37TKK237 pKa = 9.91GGTVPFLARR246 pKa = 11.84SILITSNQAPQEE258 pKa = 4.44WYY260 pKa = 10.74SSTAVPAVEE269 pKa = 3.43ALYY272 pKa = 10.72RR273 pKa = 11.84RR274 pKa = 11.84ITTLQFWKK282 pKa = 9.09TAGEE286 pKa = 4.08QSTEE290 pKa = 3.96VPEE293 pKa = 4.57GRR295 pKa = 11.84FEE297 pKa = 5.48AVDD300 pKa = 4.0PPCALFPYY308 pKa = 9.27KK309 pKa = 10.19INYY312 pKa = 8.38

Molecular weight: 35.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q805H4-2|REP-2_PCV1 Isoform of Q805H4 Isoform Rep' of Replication-associated protein OS=Porcine circovirus 1 OX=133704 GN=Rep PE=4 SV=1
MM1 pKa = 6.52TWPRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84YY8 pKa = 8.64RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84TRR14 pKa = 11.84PRR16 pKa = 11.84SHH18 pKa = 7.24LGNILRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84PYY28 pKa = 9.6LAHH31 pKa = 6.14PAFRR35 pKa = 11.84NRR37 pKa = 11.84YY38 pKa = 5.9RR39 pKa = 11.84WRR41 pKa = 11.84RR42 pKa = 11.84KK43 pKa = 7.69TGIFNSRR50 pKa = 11.84LSTEE54 pKa = 3.98LVLTIKK60 pKa = 10.55GGYY63 pKa = 7.57SQPSWNVNYY72 pKa = 10.68LKK74 pKa = 10.9FNIGQFLPPSGGTNPLPLPFQYY96 pKa = 11.0YY97 pKa = 10.16RR98 pKa = 11.84IRR100 pKa = 11.84KK101 pKa = 8.4AKK103 pKa = 9.93YY104 pKa = 7.89EE105 pKa = 4.13FYY107 pKa = 10.29PRR109 pKa = 11.84DD110 pKa = 4.08PITSNEE116 pKa = 4.04RR117 pKa = 11.84GVGSTVVILDD127 pKa = 3.79ANFVTPSTNLAYY139 pKa = 10.19DD140 pKa = 3.7PYY142 pKa = 10.12INYY145 pKa = 10.06SSRR148 pKa = 11.84HH149 pKa = 5.27TIRR152 pKa = 11.84QPFTYY157 pKa = 9.31HH158 pKa = 5.9SRR160 pKa = 11.84YY161 pKa = 7.46FTPKK165 pKa = 9.93PEE167 pKa = 4.53LDD169 pKa = 3.34QTIDD173 pKa = 3.01WFHH176 pKa = 7.17PNNKK180 pKa = 9.16RR181 pKa = 11.84NQLWLHH187 pKa = 6.5LNTHH191 pKa = 6.09TNVEE195 pKa = 4.43HH196 pKa = 6.47TGLGYY201 pKa = 11.1ALQNAATAQNYY212 pKa = 7.12VVRR215 pKa = 11.84LTIYY219 pKa = 8.42VQFRR223 pKa = 11.84EE224 pKa = 5.18FILKK228 pKa = 10.47DD229 pKa = 3.32PP230 pKa = 4.57

Molecular weight: 27.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

4

6

987

73

312

164.5

18.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.282 ± 1.017

1.925 ± 0.711

2.736 ± 0.504

6.586 ± 1.073

4.559 ± 0.393

6.079 ± 0.77

2.229 ± 0.428

4.255 ± 0.43

6.586 ± 0.858

7.295 ± 0.539

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.811 ± 0.141

4.559 ± 0.904

8.105 ± 0.808

4.661 ± 0.351

6.991 ± 1.362

6.788 ± 0.654

6.991 ± 0.722

5.066 ± 0.743

2.533 ± 0.346

4.965 ± 0.71
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski