Prevotella micans F0438
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2058 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H1Q3Q5|H1Q3Q5_9BACT 30S ribosomal protein S21 OS=Prevotella micans F0438 OX=883158 GN=rpsU PE=3 SV=1
MM1 pKa = 6.31 NQKK4 pKa = 9.43 YY5 pKa = 9.23 VCDD8 pKa = 3.79 VCGWVYY14 pKa = 11.2 DD15 pKa = 4.2 PAVGDD20 pKa = 3.94 PDD22 pKa = 4.21 NGIEE26 pKa = 4.59 PGTPFDD32 pKa = 5.06 KK33 pKa = 11.11 LPEE36 pKa = 4.3 DD37 pKa = 4.06 WVCPEE42 pKa = 4.19 CGVGKK47 pKa = 10.38 SEE49 pKa = 4.94 FSPLEE54 pKa = 4.05 DD55 pKa = 3.19
Molecular weight: 6.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.829
IPC2_protein 3.706
IPC_protein 3.605
Toseland 3.414
ProMoST 3.706
Dawson 3.617
Bjellqvist 3.872
Wikipedia 3.592
Rodwell 3.452
Grimsley 3.338
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 3.948
Thurlkill 3.503
EMBOSS 3.592
Sillero 3.745
Patrickios 0.299
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.781
Protein with the highest isoelectric point:
>tr|H1Q3H1|H1Q3H1_9BACT ATP synthase subunit delta OS=Prevotella micans F0438 OX=883158 GN=atpH PE=3 SV=1
MM1 pKa = 7.85 PNGKK5 pKa = 9.19 KK6 pKa = 10.25 KK7 pKa = 10.12 KK8 pKa = 7.0 GHH10 pKa = 6.14 KK11 pKa = 9.06 MATHH15 pKa = 6.13 KK16 pKa = 10.39 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.25 NRR25 pKa = 11.84 HH26 pKa = 4.69 KK27 pKa = 11.1 SKK29 pKa = 11.1
Molecular weight: 3.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.361
IPC2_protein 10.862
IPC_protein 12.325
Toseland 12.53
ProMoST 12.983
Dawson 12.544
Bjellqvist 12.501
Wikipedia 12.983
Rodwell 12.647
Grimsley 12.574
Solomon 12.998
Lehninger 12.91
Nozaki 12.53
DTASelect 12.501
Thurlkill 12.53
EMBOSS 13.013
Sillero 12.53
Patrickios 12.369
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.904
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2058
0
2058
708508
29
2518
344.3
38.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.138 ± 0.051
1.168 ± 0.021
5.451 ± 0.037
6.16 ± 0.061
4.542 ± 0.035
6.714 ± 0.052
2.036 ± 0.024
7.232 ± 0.047
6.422 ± 0.054
8.786 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.703 ± 0.026
5.402 ± 0.047
3.868 ± 0.03
3.627 ± 0.034
5.175 ± 0.043
6.25 ± 0.043
6.0 ± 0.044
6.151 ± 0.044
1.15 ± 0.02
4.025 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here