Torque teno Tadarida brasiliensis virus
Average proteome isoelectric point is 7.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A088MWS2|A0A088MWS2_9VIRU Capsid protein OS=Torque teno Tadarida brasiliensis virus OX=1543419 PE=3 SV=1
MM1 pKa = 7.88 GDD3 pKa = 3.51 FPVPPKK9 pKa = 10.84 GLAHH13 pKa = 5.84 YY14 pKa = 8.38 WSSKK18 pKa = 8.51 LWASGTLAAHH28 pKa = 6.99 NSFCTCADD36 pKa = 3.7 PAGHH40 pKa = 7.39 LGAALSGTLAEE51 pKa = 4.93 FFKK54 pKa = 10.81 EE55 pKa = 4.11 KK56 pKa = 10.58 CQSTKK61 pKa = 10.98 DD62 pKa = 3.56 GASPEE67 pKa = 4.23 GLLTTGDD74 pKa = 3.69 EE75 pKa = 4.53 DD76 pKa = 4.96 GSGEE80 pKa = 4.16 GALDD84 pKa = 3.65 LLIEE88 pKa = 4.34 AFEE91 pKa = 4.98 GDD93 pKa = 3.38 TAGDD97 pKa = 3.59 TKK99 pKa = 11.36
Molecular weight: 10.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.389
IPC2_protein 4.418
IPC_protein 4.304
Toseland 4.139
ProMoST 4.444
Dawson 4.279
Bjellqvist 4.431
Wikipedia 4.177
Rodwell 4.151
Grimsley 4.05
Solomon 4.266
Lehninger 4.228
Nozaki 4.393
DTASelect 4.571
Thurlkill 4.164
EMBOSS 4.19
Sillero 4.431
Patrickios 3.567
IPC_peptide 4.266
IPC2_peptide 4.406
IPC2.peptide.svr19 4.36
Protein with the highest isoelectric point:
>tr|A0A088MK20|A0A088MK20_9VIRU ORF2 OS=Torque teno Tadarida brasiliensis virus OX=1543419 PE=4 SV=1
MM1 pKa = 7.07 QQTQPLIQFQNHH13 pKa = 5.27 IQTACNTPPEE23 pKa = 4.43 PVQSYY28 pKa = 10.25 SRR30 pKa = 11.84 YY31 pKa = 9.67 KK32 pKa = 10.74 SMTRR36 pKa = 11.84 VRR38 pKa = 11.84 WANQSSTHH46 pKa = 5.07 GTSGEE51 pKa = 3.87 ASSPKK56 pKa = 10.35 GLSKK60 pKa = 10.69 EE61 pKa = 3.99 LLEE64 pKa = 4.55 EE65 pKa = 4.21 LTDD68 pKa = 5.45 LLLQQHH74 pKa = 6.04 TDD76 pKa = 3.3 QLEE79 pKa = 4.19 KK80 pKa = 10.89 KK81 pKa = 9.56 QGKK84 pKa = 9.22 SSQKK88 pKa = 10.05 KK89 pKa = 8.68 KK90 pKa = 10.19 HH91 pKa = 5.17 PQKK94 pKa = 10.36 KK95 pKa = 8.76 QRR97 pKa = 11.84 AHH99 pKa = 4.02 QTRR102 pKa = 11.84 KK103 pKa = 9.29 RR104 pKa = 11.84 RR105 pKa = 11.84 RR106 pKa = 11.84 SVAAQKK112 pKa = 10.63 EE113 pKa = 4.67 EE114 pKa = 4.26 NSSSTNWSEE123 pKa = 4.72 SSASDD128 pKa = 2.9 WDD130 pKa = 3.87 FF131 pKa = 4.08
Molecular weight: 15.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.975
IPC2_protein 9.107
IPC_protein 9.048
Toseland 10.16
ProMoST 9.633
Dawson 10.248
Bjellqvist 9.838
Wikipedia 10.35
Rodwell 10.906
Grimsley 10.277
Solomon 10.292
Lehninger 10.277
Nozaki 10.131
DTASelect 9.823
Thurlkill 10.131
EMBOSS 10.526
Sillero 10.16
Patrickios 10.672
IPC_peptide 10.292
IPC2_peptide 8.244
IPC2.peptide.svr19 8.133
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
777
99
547
259.0
29.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.692 ± 1.343
0.901 ± 0.483
5.148 ± 0.678
5.277 ± 0.848
3.218 ± 0.5
6.564 ± 1.538
3.346 ± 0.135
4.376 ± 1.548
6.306 ± 1.194
8.237 ± 0.585
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.544 ± 0.13
3.346 ± 0.489
5.792 ± 0.526
6.049 ± 1.981
7.465 ± 2.058
8.108 ± 2.161
7.593 ± 0.124
2.831 ± 0.553
3.604 ± 0.72
3.604 ± 1.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here