[Haemophilus] felis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Pasteurellaceae incertae sedis

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T0AVV9|A0A1T0AVV9_9PAST IS200/IS605 family transposase OS=[Haemophilus] felis OX=123822 GN=B0188_10490 PE=4 SV=1
MM1 pKa = 7.27AVVGIFYY8 pKa = 10.72GSDD11 pKa = 2.82TGNTEE16 pKa = 4.47NIAKK20 pKa = 9.24MIQKK24 pKa = 9.51QLGEE28 pKa = 4.21EE29 pKa = 4.12LVDD32 pKa = 3.48VRR34 pKa = 11.84DD35 pKa = 3.59IAKK38 pKa = 8.48STKK41 pKa = 9.86EE42 pKa = 4.18DD43 pKa = 3.05IEE45 pKa = 5.79AYY47 pKa = 9.78DD48 pKa = 3.99FLMIGIPTWYY58 pKa = 9.99YY59 pKa = 11.6GEE61 pKa = 4.69AQCDD65 pKa = 3.26WDD67 pKa = 5.24DD68 pKa = 4.13FFPTLEE74 pKa = 4.64QIDD77 pKa = 4.09FTDD80 pKa = 3.2KK81 pKa = 10.68LVAIFGCGDD90 pKa = 3.25QEE92 pKa = 5.04DD93 pKa = 4.3YY94 pKa = 11.79AEE96 pKa = 4.32YY97 pKa = 10.5FCDD100 pKa = 4.34AMGTVRR106 pKa = 11.84NIIEE110 pKa = 4.26PKK112 pKa = 8.68GAIIVGHH119 pKa = 6.86WPTEE123 pKa = 4.02GYY125 pKa = 10.46NFEE128 pKa = 4.12VSQALVDD135 pKa = 4.36DD136 pKa = 4.01NTFVGLCIDD145 pKa = 4.19EE146 pKa = 4.82DD147 pKa = 3.92RR148 pKa = 11.84QPEE151 pKa = 4.15LTAQRR156 pKa = 11.84VEE158 pKa = 4.06TWVKK162 pKa = 9.69QVYY165 pKa = 10.73DD166 pKa = 4.17EE167 pKa = 4.4MCLAEE172 pKa = 4.39LAA174 pKa = 4.24

Molecular weight: 19.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T0BC41|A0A1T0BC41_9PAST Cell division protein FtsX OS=[Haemophilus] felis OX=123822 GN=B0188_00305 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84SRR14 pKa = 11.84THH16 pKa = 6.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2132

0

2132

685025

19

7145

321.3

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.353 ± 0.061

0.993 ± 0.025

5.016 ± 0.055

6.325 ± 0.06

4.306 ± 0.052

6.701 ± 0.077

2.072 ± 0.026

6.776 ± 0.053

6.344 ± 0.054

10.502 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.039

5.1 ± 0.076

3.661 ± 0.049

5.241 ± 0.064

4.342 ± 0.062

5.981 ± 0.059

5.292 ± 0.099

6.568 ± 0.051

1.09 ± 0.022

3.136 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski