Paracoccus suum
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3039 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A344PK97|A0A344PK97_9RHOB Uncharacterized protein OS=Paracoccus suum OX=2259340 GN=DRW48_08980 PE=4 SV=1
MM1 pKa = 7.29 NLSPAAPAAPAEE13 pKa = 4.28 GAPLIEE19 pKa = 5.36 PSTVDD24 pKa = 3.02 HH25 pKa = 7.11 PLYY28 pKa = 10.82 DD29 pKa = 4.46 SIVEE33 pKa = 4.24 ACRR36 pKa = 11.84 SVYY39 pKa = 10.64 DD40 pKa = 3.59 PEE42 pKa = 5.06 IPVNIFDD49 pKa = 5.17 LGLIYY54 pKa = 10.14 TIVVNDD60 pKa = 3.7 AAEE63 pKa = 3.98 VMIRR67 pKa = 11.84 MTLTAPGCPVAGEE80 pKa = 4.15 MPGWVADD87 pKa = 4.01 AVNAIPGVKK96 pKa = 9.69 QVDD99 pKa = 3.37 VHH101 pKa = 5.45 MTFEE105 pKa = 4.54 PQWGMDD111 pKa = 3.56 MMSDD115 pKa = 3.68 EE116 pKa = 4.89 ARR118 pKa = 11.84 LEE120 pKa = 4.14 LGFMM124 pKa = 4.54
Molecular weight: 13.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.897
IPC_protein 3.821
Toseland 3.63
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.541
Solomon 3.783
Lehninger 3.745
Nozaki 3.935
DTASelect 4.113
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.935
Patrickios 1.875
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.853
Protein with the highest isoelectric point:
>tr|A0A344PJD7|A0A344PJD7_9RHOB SOS response-associated peptidase OS=Paracoccus suum OX=2259340 GN=DRW48_07140 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.12 GGRR28 pKa = 11.84 RR29 pKa = 11.84 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.53 GRR39 pKa = 11.84 KK40 pKa = 8.73 VLSAA44 pKa = 4.05
Molecular weight: 5.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3039
0
3039
964569
29
2877
317.4
34.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.081 ± 0.075
0.782 ± 0.014
5.863 ± 0.038
5.357 ± 0.038
3.319 ± 0.026
9.159 ± 0.051
1.922 ± 0.021
4.867 ± 0.03
2.482 ± 0.039
10.141 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.65 ± 0.021
2.252 ± 0.023
5.786 ± 0.035
3.079 ± 0.023
7.44 ± 0.047
4.803 ± 0.029
5.376 ± 0.029
7.261 ± 0.039
1.426 ± 0.018
1.954 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here