Mycobacterium phage Acolyte

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345KUX2|A0A345KUX2_9CAUD Uncharacterized protein OS=Mycobacterium phage Acolyte OX=2250290 GN=81 PE=4 SV=1
MM1 pKa = 7.32NRR3 pKa = 11.84TSTNVLLSLLAAFTAVGAMLLADD26 pKa = 4.14PAEE29 pKa = 4.73GSTGPLEE36 pKa = 5.54DD37 pKa = 3.95EE38 pKa = 5.2PGWSCVDD45 pKa = 3.72DD46 pKa = 4.2GNRR49 pKa = 11.84ICGPGNDD56 pKa = 3.67EE57 pKa = 4.62GKK59 pKa = 9.54PAGCYY64 pKa = 10.03DD65 pKa = 3.85DD66 pKa = 5.92GGVLVAEE73 pKa = 5.18WPCKK77 pKa = 9.63AWQPSDD83 pKa = 3.63GYY85 pKa = 10.75RR86 pKa = 11.84HH87 pKa = 6.49GDD89 pKa = 3.4GTVTYY94 pKa = 9.13PDD96 pKa = 4.09PDD98 pKa = 3.55EE99 pKa = 4.39PVYY102 pKa = 10.21LTGYY106 pKa = 8.53TNN108 pKa = 3.37

Molecular weight: 11.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345KUY6|A0A345KUY6_9CAUD Uncharacterized protein OS=Mycobacterium phage Acolyte OX=2250290 GN=93 PE=4 SV=1
MM1 pKa = 7.76TYY3 pKa = 10.44RR4 pKa = 11.84FPEE7 pKa = 3.87EE8 pKa = 3.77SMRR11 pKa = 11.84RR12 pKa = 11.84YY13 pKa = 9.78LVLSAQVLPKK23 pKa = 10.43GIEE26 pKa = 3.76EE27 pKa = 4.15ARR29 pKa = 11.84PLSRR33 pKa = 11.84SSHH36 pKa = 5.01MKK38 pKa = 10.0PYY40 pKa = 9.54SHH42 pKa = 7.35WVPGLTAPRR51 pKa = 11.84RR52 pKa = 11.84AGDD55 pKa = 3.41SHH57 pKa = 5.25STIWSRR63 pKa = 11.84WLSSGPCWYY72 pKa = 10.23SQRR75 pKa = 11.84CPRR78 pKa = 11.84YY79 pKa = 9.1RR80 pKa = 11.84RR81 pKa = 11.84WLKK84 pKa = 10.49AVPAQLTGPSWW95 pKa = 3.21

Molecular weight: 11.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

16659

25

983

181.1

19.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.049 ± 0.333

0.936 ± 0.128

6.255 ± 0.25

6.387 ± 0.384

3.278 ± 0.16

8.41 ± 0.517

2.245 ± 0.18

4.892 ± 0.175

4.574 ± 0.234

8.464 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.389 ± 0.117

3.157 ± 0.174

5.493 ± 0.259

3.77 ± 0.274

6.267 ± 0.349

5.384 ± 0.251

6.153 ± 0.285

7.035 ± 0.208

1.975 ± 0.103

2.887 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski