Amino acid dipepetide frequency for Mycobacterium phage Acolyte

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.166AlaAla: 11.166 ± 1.111
0.6AlaCys: 0.6 ± 0.159
5.823AlaAsp: 5.823 ± 0.67
6.603AlaGlu: 6.603 ± 0.705
3.962AlaPhe: 3.962 ± 0.453
9.245AlaGly: 9.245 ± 1.024
1.681AlaHis: 1.681 ± 0.3
4.562AlaIle: 4.562 ± 0.526
5.163AlaLys: 5.163 ± 0.676
9.485AlaLeu: 9.485 ± 0.852
2.581AlaMet: 2.581 ± 0.356
2.521AlaAsn: 2.521 ± 0.399
3.842AlaPro: 3.842 ± 0.466
3.902AlaGln: 3.902 ± 0.533
5.643AlaArg: 5.643 ± 0.563
5.883AlaSer: 5.883 ± 0.494
5.943AlaThr: 5.943 ± 0.715
7.084AlaVal: 7.084 ± 0.665
2.221AlaTrp: 2.221 ± 0.376
2.581AlaTyr: 2.581 ± 0.449
0.0AlaXaa: 0.0 ± 0.0
Cys
0.6CysAla: 0.6 ± 0.18
0.06CysCys: 0.06 ± 0.064
0.78CysAsp: 0.78 ± 0.207
0.54CysGlu: 0.54 ± 0.165
0.24CysPhe: 0.24 ± 0.136
0.72CysGly: 0.72 ± 0.214
0.24CysHis: 0.24 ± 0.131
0.18CysIle: 0.18 ± 0.095
0.42CysLys: 0.42 ± 0.155
0.72CysLeu: 0.72 ± 0.226
0.24CysMet: 0.24 ± 0.147
0.36CysAsn: 0.36 ± 0.126
0.78CysPro: 0.78 ± 0.265
0.18CysGln: 0.18 ± 0.085
0.6CysArg: 0.6 ± 0.183
0.36CysSer: 0.36 ± 0.131
0.54CysThr: 0.54 ± 0.166
0.72CysVal: 0.72 ± 0.216
0.54CysTrp: 0.54 ± 0.163
0.54CysTyr: 0.54 ± 0.174
0.0CysXaa: 0.0 ± 0.0
Asp
5.883AspAla: 5.883 ± 0.649
0.84AspCys: 0.84 ± 0.238
3.842AspAsp: 3.842 ± 0.562
4.262AspGlu: 4.262 ± 0.591
2.521AspPhe: 2.521 ± 0.456
6.423AspGly: 6.423 ± 0.683
1.741AspHis: 1.741 ± 0.34
3.242AspIle: 3.242 ± 0.43
2.221AspLys: 2.221 ± 0.434
5.523AspLeu: 5.523 ± 0.566
1.621AspMet: 1.621 ± 0.338
1.621AspAsn: 1.621 ± 0.258
5.043AspPro: 5.043 ± 0.635
1.981AspGln: 1.981 ± 0.347
3.122AspArg: 3.122 ± 0.389
2.821AspSer: 2.821 ± 0.393
3.182AspThr: 3.182 ± 0.406
3.602AspVal: 3.602 ± 0.438
1.081AspTrp: 1.081 ± 0.269
1.981AspTyr: 1.981 ± 0.282
0.0AspXaa: 0.0 ± 0.0
Glu
7.684GluAla: 7.684 ± 0.865
0.24GluCys: 0.24 ± 0.117
3.902GluAsp: 3.902 ± 0.526
5.043GluGlu: 5.043 ± 0.706
2.521GluPhe: 2.521 ± 0.373
4.802GluGly: 4.802 ± 0.623
1.621GluHis: 1.621 ± 0.396
3.482GluIle: 3.482 ± 0.411
2.821GluLys: 2.821 ± 0.368
7.684GluLeu: 7.684 ± 0.869
1.681GluMet: 1.681 ± 0.345
1.321GluAsn: 1.321 ± 0.25
2.761GluPro: 2.761 ± 0.399
1.861GluGln: 1.861 ± 0.339
3.542GluArg: 3.542 ± 0.589
2.701GluSer: 2.701 ± 0.342
3.362GluThr: 3.362 ± 0.423
3.782GluVal: 3.782 ± 0.477
1.261GluTrp: 1.261 ± 0.306
1.801GluTyr: 1.801 ± 0.321
0.0GluXaa: 0.0 ± 0.0
Phe
3.362PheAla: 3.362 ± 0.344
0.24PheCys: 0.24 ± 0.12
2.341PheAsp: 2.341 ± 0.458
2.581PheGlu: 2.581 ± 0.372
0.66PhePhe: 0.66 ± 0.161
3.122PheGly: 3.122 ± 0.419
0.66PheHis: 0.66 ± 0.224
1.441PheIle: 1.441 ± 0.26
1.081PheLys: 1.081 ± 0.334
1.981PheLeu: 1.981 ± 0.507
0.42PheMet: 0.42 ± 0.127
1.501PheAsn: 1.501 ± 0.283
1.621PhePro: 1.621 ± 0.331
1.081PheGln: 1.081 ± 0.32
2.161PheArg: 2.161 ± 0.36
1.741PheSer: 1.741 ± 0.284
2.641PheThr: 2.641 ± 0.338
2.701PheVal: 2.701 ± 0.452
0.48PheTrp: 0.48 ± 0.162
0.96PheTyr: 0.96 ± 0.218
0.0PheXaa: 0.0 ± 0.0
Gly
6.964GlyAla: 6.964 ± 0.877
0.72GlyCys: 0.72 ± 0.215
5.823GlyAsp: 5.823 ± 0.589
5.103GlyGlu: 5.103 ± 0.516
3.542GlyPhe: 3.542 ± 0.471
8.765GlyGly: 8.765 ± 1.85
2.221GlyHis: 2.221 ± 0.36
3.962GlyIle: 3.962 ± 0.597
3.662GlyLys: 3.662 ± 0.55
5.943GlyLeu: 5.943 ± 0.63
1.681GlyMet: 1.681 ± 0.327
3.302GlyAsn: 3.302 ± 0.555
4.262GlyPro: 4.262 ± 0.437
3.122GlyGln: 3.122 ± 0.403
4.022GlyArg: 4.022 ± 0.43
4.622GlySer: 4.622 ± 0.855
6.063GlyThr: 6.063 ± 0.805
5.943GlyVal: 5.943 ± 0.586
1.501GlyTrp: 1.501 ± 0.249
2.881GlyTyr: 2.881 ± 0.391
0.0GlyXaa: 0.0 ± 0.0
His
1.921HisAla: 1.921 ± 0.337
0.3HisCys: 0.3 ± 0.119
1.261HisAsp: 1.261 ± 0.298
1.681HisGlu: 1.681 ± 0.339
0.42HisPhe: 0.42 ± 0.155
2.161HisGly: 2.161 ± 0.511
0.54HisHis: 0.54 ± 0.159
1.321HisIle: 1.321 ± 0.302
1.261HisLys: 1.261 ± 0.281
1.861HisLeu: 1.861 ± 0.363
0.24HisMet: 0.24 ± 0.124
0.66HisAsn: 0.66 ± 0.172
1.261HisPro: 1.261 ± 0.216
0.72HisGln: 0.72 ± 0.206
1.801HisArg: 1.801 ± 0.381
0.96HisSer: 0.96 ± 0.26
1.381HisThr: 1.381 ± 0.278
1.561HisVal: 1.561 ± 0.244
0.36HisTrp: 0.36 ± 0.159
0.78HisTyr: 0.78 ± 0.261
0.0HisXaa: 0.0 ± 0.0
Ile
5.403IleAla: 5.403 ± 0.661
0.66IleCys: 0.66 ± 0.179
3.062IleAsp: 3.062 ± 0.396
3.962IleGlu: 3.962 ± 0.458
1.441IlePhe: 1.441 ± 0.361
4.202IleGly: 4.202 ± 0.43
1.141IleHis: 1.141 ± 0.259
1.501IleIle: 1.501 ± 0.311
2.281IleLys: 2.281 ± 0.38
4.742IleLeu: 4.742 ± 0.505
0.42IleMet: 0.42 ± 0.15
1.381IleAsn: 1.381 ± 0.301
3.662IlePro: 3.662 ± 0.445
1.741IleGln: 1.741 ± 0.315
2.821IleArg: 2.821 ± 0.37
2.942IleSer: 2.942 ± 0.442
3.302IleThr: 3.302 ± 0.496
2.641IleVal: 2.641 ± 0.389
0.96IleTrp: 0.96 ± 0.208
0.66IleTyr: 0.66 ± 0.193
0.0IleXaa: 0.0 ± 0.0
Lys
5.763LysAla: 5.763 ± 0.691
0.3LysCys: 0.3 ± 0.133
2.701LysAsp: 2.701 ± 0.418
2.221LysGlu: 2.221 ± 0.408
0.9LysPhe: 0.9 ± 0.244
3.362LysGly: 3.362 ± 0.436
0.54LysHis: 0.54 ± 0.167
2.161LysIle: 2.161 ± 0.379
2.521LysLys: 2.521 ± 0.447
3.722LysLeu: 3.722 ± 0.61
0.96LysMet: 0.96 ± 0.183
1.501LysAsn: 1.501 ± 0.302
3.002LysPro: 3.002 ± 0.455
1.501LysGln: 1.501 ± 0.287
3.122LysArg: 3.122 ± 0.463
2.521LysSer: 2.521 ± 0.426
2.881LysThr: 2.881 ± 0.432
3.902LysVal: 3.902 ± 0.542
0.96LysTrp: 0.96 ± 0.236
1.201LysTyr: 1.201 ± 0.281
0.0LysXaa: 0.0 ± 0.0
Leu
9.065LeuAla: 9.065 ± 0.765
0.72LeuCys: 0.72 ± 0.18
5.043LeuAsp: 5.043 ± 0.614
4.923LeuGlu: 4.923 ± 0.605
2.341LeuPhe: 2.341 ± 0.392
6.423LeuGly: 6.423 ± 0.767
2.221LeuHis: 2.221 ± 0.407
4.742LeuIle: 4.742 ± 0.582
3.902LeuLys: 3.902 ± 0.528
6.303LeuLeu: 6.303 ± 0.691
2.521LeuMet: 2.521 ± 0.351
2.641LeuAsn: 2.641 ± 0.376
5.163LeuPro: 5.163 ± 0.461
3.242LeuGln: 3.242 ± 0.526
5.823LeuArg: 5.823 ± 0.604
5.463LeuSer: 5.463 ± 0.639
4.562LeuThr: 4.562 ± 0.497
5.943LeuVal: 5.943 ± 0.834
1.621LeuTrp: 1.621 ± 0.289
1.921LeuTyr: 1.921 ± 0.387
0.0LeuXaa: 0.0 ± 0.0
Met
2.581MetAla: 2.581 ± 0.427
0.06MetCys: 0.06 ± 0.058
0.9MetAsp: 0.9 ± 0.232
0.9MetGlu: 0.9 ± 0.185
0.54MetPhe: 0.54 ± 0.214
1.981MetGly: 1.981 ± 0.395
0.54MetHis: 0.54 ± 0.148
1.501MetIle: 1.501 ± 0.352
1.441MetLys: 1.441 ± 0.276
1.381MetLeu: 1.381 ± 0.294
0.6MetMet: 0.6 ± 0.192
0.78MetAsn: 0.78 ± 0.176
1.261MetPro: 1.261 ± 0.282
1.261MetGln: 1.261 ± 0.266
1.561MetArg: 1.561 ± 0.324
2.041MetSer: 2.041 ± 0.308
2.461MetThr: 2.461 ± 0.317
1.261MetVal: 1.261 ± 0.268
0.12MetTrp: 0.12 ± 0.086
0.72MetTyr: 0.72 ± 0.244
0.0MetXaa: 0.0 ± 0.0
Asn
2.942AsnAla: 2.942 ± 0.403
0.42AsnCys: 0.42 ± 0.139
2.101AsnAsp: 2.101 ± 0.358
2.341AsnGlu: 2.341 ± 0.321
0.6AsnPhe: 0.6 ± 0.154
3.062AsnGly: 3.062 ± 0.426
1.141AsnHis: 1.141 ± 0.224
1.621AsnIle: 1.621 ± 0.314
1.261AsnLys: 1.261 ± 0.221
2.401AsnLeu: 2.401 ± 0.361
0.84AsnMet: 0.84 ± 0.203
0.36AsnAsn: 0.36 ± 0.153
2.101AsnPro: 2.101 ± 0.341
0.9AsnGln: 0.9 ± 0.228
2.341AsnArg: 2.341 ± 0.433
1.321AsnSer: 1.321 ± 0.343
1.921AsnThr: 1.921 ± 0.453
2.341AsnVal: 2.341 ± 0.381
0.54AsnTrp: 0.54 ± 0.169
1.021AsnTyr: 1.021 ± 0.244
0.0AsnXaa: 0.0 ± 0.0
Pro
5.343ProAla: 5.343 ± 0.545
0.54ProCys: 0.54 ± 0.175
4.142ProAsp: 4.142 ± 0.559
4.502ProGlu: 4.502 ± 0.628
1.681ProPhe: 1.681 ± 0.432
4.262ProGly: 4.262 ± 0.534
1.081ProHis: 1.081 ± 0.23
2.942ProIle: 2.942 ± 0.359
2.281ProLys: 2.281 ± 0.459
4.322ProLeu: 4.322 ± 0.486
1.021ProMet: 1.021 ± 0.232
2.221ProAsn: 2.221 ± 0.451
2.161ProPro: 2.161 ± 0.602
1.741ProGln: 1.741 ± 0.365
3.062ProArg: 3.062 ± 0.473
3.002ProSer: 3.002 ± 0.445
4.022ProThr: 4.022 ± 0.561
4.022ProVal: 4.022 ± 0.532
1.201ProTrp: 1.201 ± 0.347
1.381ProTyr: 1.381 ± 0.33
0.0ProXaa: 0.0 ± 0.0
Gln
4.382GlnAla: 4.382 ± 0.495
0.3GlnCys: 0.3 ± 0.12
1.441GlnAsp: 1.441 ± 0.239
1.501GlnGlu: 1.501 ± 0.349
1.741GlnPhe: 1.741 ± 0.291
2.521GlnGly: 2.521 ± 0.339
0.84GlnHis: 0.84 ± 0.203
2.521GlnIle: 2.521 ± 0.427
1.621GlnLys: 1.621 ± 0.302
3.602GlnLeu: 3.602 ± 0.749
1.201GlnMet: 1.201 ± 0.268
1.141GlnAsn: 1.141 ± 0.247
1.561GlnPro: 1.561 ± 0.394
2.521GlnGln: 2.521 ± 0.657
2.641GlnArg: 2.641 ± 0.411
1.561GlnSer: 1.561 ± 0.271
1.861GlnThr: 1.861 ± 0.345
3.062GlnVal: 3.062 ± 0.386
0.96GlnTrp: 0.96 ± 0.222
0.72GlnTyr: 0.72 ± 0.161
0.0GlnXaa: 0.0 ± 0.0
Arg
5.463ArgAla: 5.463 ± 0.642
1.021ArgCys: 1.021 ± 0.293
3.722ArgAsp: 3.722 ± 0.482
4.382ArgGlu: 4.382 ± 0.56
2.401ArgPhe: 2.401 ± 0.418
3.662ArgGly: 3.662 ± 0.456
1.741ArgHis: 1.741 ± 0.339
3.062ArgIle: 3.062 ± 0.442
2.942ArgLys: 2.942 ± 0.47
6.123ArgLeu: 6.123 ± 0.654
1.861ArgMet: 1.861 ± 0.438
2.281ArgAsn: 2.281 ± 0.381
3.122ArgPro: 3.122 ± 0.39
2.101ArgGln: 2.101 ± 0.307
5.523ArgArg: 5.523 ± 0.701
2.641ArgSer: 2.641 ± 0.405
2.701ArgThr: 2.701 ± 0.412
4.022ArgVal: 4.022 ± 0.519
1.261ArgTrp: 1.261 ± 0.25
2.641ArgTyr: 2.641 ± 0.397
0.0ArgXaa: 0.0 ± 0.0
Ser
4.322SerAla: 4.322 ± 0.485
0.6SerCys: 0.6 ± 0.205
3.782SerAsp: 3.782 ± 0.426
3.002SerGlu: 3.002 ± 0.378
1.921SerPhe: 1.921 ± 0.336
4.382SerGly: 4.382 ± 0.614
1.141SerHis: 1.141 ± 0.296
2.581SerIle: 2.581 ± 0.309
2.821SerLys: 2.821 ± 0.411
4.742SerLeu: 4.742 ± 0.688
1.681SerMet: 1.681 ± 0.327
1.681SerAsn: 1.681 ± 0.313
2.641SerPro: 2.641 ± 0.411
2.641SerGln: 2.641 ± 0.41
3.542SerArg: 3.542 ± 0.572
2.521SerSer: 2.521 ± 0.382
3.062SerThr: 3.062 ± 0.405
3.542SerVal: 3.542 ± 0.478
1.561SerTrp: 1.561 ± 0.293
1.681SerTyr: 1.681 ± 0.343
0.0SerXaa: 0.0 ± 0.0
Thr
6.243ThrAla: 6.243 ± 0.571
0.42ThrCys: 0.42 ± 0.188
3.542ThrAsp: 3.542 ± 0.589
2.701ThrGlu: 2.701 ± 0.442
2.161ThrPhe: 2.161 ± 0.332
6.303ThrGly: 6.303 ± 0.766
1.081ThrHis: 1.081 ± 0.302
2.761ThrIle: 2.761 ± 0.428
3.002ThrLys: 3.002 ± 0.429
4.682ThrLeu: 4.682 ± 0.608
1.321ThrMet: 1.321 ± 0.295
1.801ThrAsn: 1.801 ± 0.341
4.682ThrPro: 4.682 ± 0.592
2.101ThrGln: 2.101 ± 0.354
3.062ThrArg: 3.062 ± 0.465
3.242ThrSer: 3.242 ± 0.503
3.782ThrThr: 3.782 ± 0.566
5.823ThrVal: 5.823 ± 0.728
0.78ThrTrp: 0.78 ± 0.26
2.041ThrTyr: 2.041 ± 0.313
0.0ThrXaa: 0.0 ± 0.0
Val
7.324ValAla: 7.324 ± 0.804
0.72ValCys: 0.72 ± 0.229
5.163ValAsp: 5.163 ± 0.547
4.382ValGlu: 4.382 ± 0.579
2.221ValPhe: 2.221 ± 0.466
5.043ValGly: 5.043 ± 0.558
1.201ValHis: 1.201 ± 0.289
3.242ValIle: 3.242 ± 0.456
3.482ValLys: 3.482 ± 0.483
4.983ValLeu: 4.983 ± 0.557
1.501ValMet: 1.501 ± 0.298
2.942ValAsn: 2.942 ± 0.355
3.902ValPro: 3.902 ± 0.497
2.521ValGln: 2.521 ± 0.437
4.442ValArg: 4.442 ± 0.577
4.442ValSer: 4.442 ± 0.5
4.262ValThr: 4.262 ± 0.52
5.163ValVal: 5.163 ± 0.551
1.501ValTrp: 1.501 ± 0.27
1.921ValTyr: 1.921 ± 0.308
0.0ValXaa: 0.0 ± 0.0
Trp
1.381TrpAla: 1.381 ± 0.312
0.42TrpCys: 0.42 ± 0.17
1.021TrpAsp: 1.021 ± 0.249
1.381TrpGlu: 1.381 ± 0.237
0.54TrpPhe: 0.54 ± 0.15
1.501TrpGly: 1.501 ± 0.244
0.54TrpHis: 0.54 ± 0.203
0.78TrpIle: 0.78 ± 0.215
0.78TrpLys: 0.78 ± 0.197
1.441TrpLeu: 1.441 ± 0.309
0.6TrpMet: 0.6 ± 0.17
0.72TrpAsn: 0.72 ± 0.196
0.96TrpPro: 0.96 ± 0.263
1.081TrpGln: 1.081 ± 0.272
1.321TrpArg: 1.321 ± 0.269
1.141TrpSer: 1.141 ± 0.283
1.801TrpThr: 1.801 ± 0.336
1.201TrpVal: 1.201 ± 0.236
0.54TrpTrp: 0.54 ± 0.158
0.6TrpTyr: 0.6 ± 0.187
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.701TyrAla: 2.701 ± 0.402
0.18TyrCys: 0.18 ± 0.099
2.161TyrAsp: 2.161 ± 0.26
1.861TyrGlu: 1.861 ± 0.327
0.42TyrPhe: 0.42 ± 0.18
2.161TyrGly: 2.161 ± 0.341
0.54TyrHis: 0.54 ± 0.169
1.321TyrIle: 1.321 ± 0.262
0.78TyrLys: 0.78 ± 0.236
3.002TyrLeu: 3.002 ± 0.475
0.84TyrMet: 0.84 ± 0.217
0.84TyrAsn: 0.84 ± 0.185
1.141TyrPro: 1.141 ± 0.259
1.501TyrGln: 1.501 ± 0.27
2.521TyrArg: 2.521 ± 0.428
1.921TyrSer: 1.921 ± 0.335
1.801TyrThr: 1.801 ± 0.298
2.041TyrVal: 2.041 ± 0.375
0.3TyrTrp: 0.3 ± 0.135
0.84TyrTyr: 0.84 ± 0.245
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (16659 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski