Sphingomonas edaphi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A418Q0Z4|A0A418Q0Z4_9SPHN Sigma-54-dependent Fis family transcriptional regulator OS=Sphingomonas edaphi OX=2315689 GN=D3M59_00950 PE=4 SV=1
AA1 pKa = 8.32AIEE4 pKa = 4.3ALDD7 pKa = 4.02AGEE10 pKa = 4.37TPSDD14 pKa = 3.63VFTVSVSDD22 pKa = 3.54GDD24 pKa = 4.27GPLVTQSYY32 pKa = 8.16TVNLTGADD40 pKa = 3.76DD41 pKa = 5.01NIAPIANSDD50 pKa = 4.28VIWVSNNTTVTLSNDD65 pKa = 3.02VLLGNDD71 pKa = 3.19IDD73 pKa = 4.09VDD75 pKa = 4.78GIALILTNVVVTTGLLASPVVLNANGTFTFTTGAAGGTVGTPTVVTLTYY124 pKa = 10.08STDD127 pKa = 3.44DD128 pKa = 3.72GAGGTTTGSVTVNIITTTSGVDD150 pKa = 3.59SLNLTAVPAYY160 pKa = 9.22QASFIQGLAGADD172 pKa = 3.55TLTDD176 pKa = 3.49GTGVSTLLGGDD187 pKa = 3.5GADD190 pKa = 3.54NLTGNAGNDD199 pKa = 3.63LLIGGDD205 pKa = 3.81NNDD208 pKa = 3.66VLDD211 pKa = 4.62GGSGNDD217 pKa = 3.15ILRR220 pKa = 11.84GGFGNNDD227 pKa = 3.55EE228 pKa = 4.54MNGGAGSEE236 pKa = 4.24DD237 pKa = 3.96LLDD240 pKa = 4.66FSDD243 pKa = 3.65GTAAVTFTLVQSAVFTSIANGTGGLGNNDD272 pKa = 3.33KK273 pKa = 10.63YY274 pKa = 11.4MNMEE278 pKa = 3.99GVIGTSQGDD287 pKa = 3.99TLTGSANNDD296 pKa = 2.9ILRR299 pKa = 11.84GGGGNDD305 pKa = 3.39TLNGGGGLGDD315 pKa = 5.49LIDD318 pKa = 4.71FRR320 pKa = 11.84DD321 pKa = 4.04GTAGITFTLVQGGGATVFNASAAGLGSDD349 pKa = 3.8TYY351 pKa = 11.86SNIEE355 pKa = 3.95GVIGTVFNDD364 pKa = 2.96ILTGSSGNDD373 pKa = 2.96ILRR376 pKa = 11.84GDD378 pKa = 4.26GGNDD382 pKa = 3.45VINGADD388 pKa = 3.76GDD390 pKa = 4.1DD391 pKa = 3.94TLVGGAGADD400 pKa = 3.89TITGGLGNDD409 pKa = 3.45VFVLTTPLNAVDD421 pKa = 4.64TIVDD425 pKa = 3.46YY426 pKa = 11.34SGSVGNADD434 pKa = 4.28VIDD437 pKa = 3.42ITAILNVPGAVDD449 pKa = 4.98PINDD453 pKa = 3.78GYY455 pKa = 11.61LRR457 pKa = 11.84VTTTGLLQVDD467 pKa = 4.77LDD469 pKa = 4.09GGGNNWITLANINTGVSPTVRR490 pKa = 11.84YY491 pKa = 9.59IDD493 pKa = 3.78GGVITNIVVAPVAPPIALDD512 pKa = 3.63LDD514 pKa = 3.86GDD516 pKa = 4.35GFVSFTGTDD525 pKa = 3.01GGAVFDD531 pKa = 4.27YY532 pKa = 11.01GYY534 pKa = 9.16GTVSTAWVAGNDD546 pKa = 4.39GILVRR551 pKa = 11.84DD552 pKa = 3.9ANGDD556 pKa = 3.38GHH558 pKa = 7.06ISSNEE563 pKa = 3.5IVFATTGSDD572 pKa = 3.63LEE574 pKa = 4.45GLATYY579 pKa = 7.57DD580 pKa = 3.74TNQDD584 pKa = 3.57GQLSAADD591 pKa = 3.53SQFAEE596 pKa = 4.37FGVWQDD602 pKa = 3.36ADD604 pKa = 3.4SDD606 pKa = 4.32GQVDD610 pKa = 3.58AGEE613 pKa = 4.26LQSLAAHH620 pKa = 6.87SIASISLSSDD630 pKa = 2.71GVAYY634 pKa = 10.44SAANGDD640 pKa = 3.86VQVVGTGSYY649 pKa = 9.7TRR651 pKa = 11.84TDD653 pKa = 3.01GSTGVLADD661 pKa = 3.76AVFATGNQLAANDD674 pKa = 4.03PRR676 pKa = 11.84LAPAAGNTAILAAAAAAVGLASMPAHH702 pKa = 6.56AQPADD707 pKa = 3.63SEE709 pKa = 4.65ADD711 pKa = 3.21GSFRR715 pKa = 11.84GEE717 pKa = 3.99LQEE720 pKa = 4.0TLILRR725 pKa = 11.84SHH727 pKa = 7.13ADD729 pKa = 2.9WTGLSNAASDD739 pKa = 4.21VSGLHH744 pKa = 6.76ADD746 pKa = 4.18GLASFYY752 pKa = 10.54PAMPDD757 pKa = 3.44PSQMGATPSGSNHH770 pKa = 5.81SLIGSEE776 pKa = 4.02VAVRR780 pKa = 11.84AEE782 pKa = 3.71AAFIPMEE789 pKa = 4.07MSEE792 pKa = 4.27AVHH795 pKa = 6.15VSSAPVAMAMVAMPSAEE812 pKa = 3.97LLAAAVHH819 pKa = 6.38GSGAQPHH826 pKa = 5.68GQVAAVIADD835 pKa = 4.03AFAGDD840 pKa = 3.78SHH842 pKa = 6.31ATAIDD847 pKa = 3.42ALLDD851 pKa = 3.83VVTSQHH857 pKa = 5.89NADD860 pKa = 3.58GSGGGSASLAHH871 pKa = 6.75LASAPWLAAPEE882 pKa = 3.98YY883 pKa = 10.09GQLALHH889 pKa = 5.88MQHH892 pKa = 7.15AEE894 pKa = 4.1LLVAHH899 pKa = 7.28PDD901 pKa = 3.63VLPPTT906 pKa = 4.2

Molecular weight: 89.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A418Q465|A0A418Q465_9SPHN CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase OS=Sphingomonas edaphi OX=2315689 GN=pgsA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.07VLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2311

0

2311

728684

37

2143

315.3

34.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.767 ± 0.077

0.747 ± 0.015

6.017 ± 0.036

5.815 ± 0.051

3.496 ± 0.031

8.865 ± 0.064

1.903 ± 0.023

5.096 ± 0.031

3.4 ± 0.043

9.95 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.021

2.632 ± 0.036

5.201 ± 0.039

3.097 ± 0.024

7.354 ± 0.06

5.53 ± 0.039

5.025 ± 0.038

7.224 ± 0.038

1.365 ± 0.021

2.118 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski