Xanthomarina gelatinilytica
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2841 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M7N6T0|M7N6T0_9FLAO HTH cro/C1-type domain-containing protein OS=Xanthomarina gelatinilytica OX=1137281 GN=D778_01145 PE=3 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.4 KK3 pKa = 9.73 ILLTFLSFNAIIFAGFSQEE22 pKa = 3.67 EE23 pKa = 4.67 GFAIHH28 pKa = 6.31 TVGPTGSNPYY38 pKa = 10.32 VIDD41 pKa = 4.14 SGHH44 pKa = 7.07 LNPGAYY50 pKa = 8.79 PDD52 pKa = 4.4 LAVGTQSGNTVEE64 pKa = 5.12 IYY66 pKa = 10.55 INNEE70 pKa = 3.93 DD71 pKa = 3.65 GTFAAPIIKK80 pKa = 10.01 NLSLVSGVHH89 pKa = 5.98 IADD92 pKa = 3.08 IDD94 pKa = 4.45 GINGNDD100 pKa = 3.37 VLASSSGGSKK110 pKa = 10.23 LVWYY114 pKa = 9.97 ANNGDD119 pKa = 3.69 GTFADD124 pKa = 4.07 EE125 pKa = 5.29 AIISSSLDD133 pKa = 3.38 TPGTIVTGYY142 pKa = 9.61 IDD144 pKa = 4.34 SDD146 pKa = 3.56 ATLDD150 pKa = 3.51 IVLSVYY156 pKa = 10.08 GASGDD161 pKa = 3.54 TDD163 pKa = 3.54 RR164 pKa = 11.84 VIWFANDD171 pKa = 3.36 GLPWTEE177 pKa = 3.5 QDD179 pKa = 4.05 VVPATAGLGCGNIDD193 pKa = 3.58 IADD196 pKa = 3.63 VDD198 pKa = 4.18 GDD200 pKa = 3.83 TDD202 pKa = 4.83 LDD204 pKa = 3.51 IVVANTDD211 pKa = 3.01 AGTVEE216 pKa = 5.4 LYY218 pKa = 10.84 YY219 pKa = 11.23 NNYY222 pKa = 9.77 NPLTDD227 pKa = 3.92 NNPVSFTEE235 pKa = 4.21 SAGGDD240 pKa = 2.85 ISTGNTYY247 pKa = 10.95 LFGLDD252 pKa = 3.89 FGDD255 pKa = 4.13 VNDD258 pKa = 4.94 DD259 pKa = 3.42 SFMDD263 pKa = 3.51 IVKK266 pKa = 10.09 VDD268 pKa = 3.85 LSGHH272 pKa = 6.33 EE273 pKa = 3.53 IAYY276 pKa = 7.89 YY277 pKa = 10.55 QNDD280 pKa = 3.92 GAGNFTEE287 pKa = 4.8 VPVSSHH293 pKa = 5.8 QYY295 pKa = 8.99 PSIAFVTDD303 pKa = 4.1 LNNDD307 pKa = 3.81 GYY309 pKa = 11.48 NDD311 pKa = 3.18 IVAADD316 pKa = 4.25 GLNQNDD322 pKa = 3.29 DD323 pKa = 4.05 MFWFEE328 pKa = 4.13 SDD330 pKa = 3.08 AFGGLGAEE338 pKa = 4.44 TIITNNTLHH347 pKa = 5.78 NQVYY351 pKa = 10.17 NFTINDD357 pKa = 3.54 FDD359 pKa = 4.42 NDD361 pKa = 3.51 GDD363 pKa = 3.93 IDD365 pKa = 4.19 FATLGYY371 pKa = 10.26 QDD373 pKa = 4.99 GTLKK377 pKa = 10.38 WIEE380 pKa = 4.13 NNLNLIGTQEE390 pKa = 4.04 FKK392 pKa = 11.38 LNTVSIYY399 pKa = 10.38 PNPTSNRR406 pKa = 11.84 IYY408 pKa = 10.83 FKK410 pKa = 10.9 GLTSNTEE417 pKa = 3.75 RR418 pKa = 11.84 VSVYY422 pKa = 10.62 DD423 pKa = 3.45 ILGKK427 pKa = 10.57 KK428 pKa = 9.39 ILSKK432 pKa = 9.74 TVNTNKK438 pKa = 10.45 GLDD441 pKa = 3.5 VSKK444 pKa = 10.1 LQNGIYY450 pKa = 9.79 ILKK453 pKa = 9.96 LDD455 pKa = 4.43 QLNTSYY461 pKa = 11.5 KK462 pKa = 9.75 FVKK465 pKa = 10.24 KK466 pKa = 10.63
Molecular weight: 50.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.676
IPC2_protein 3.783
IPC_protein 3.834
Toseland 3.592
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.656
Grimsley 3.49
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.253
Thurlkill 3.656
EMBOSS 3.808
Sillero 3.961
Patrickios 1.392
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|M7N096|M7N096_9FLAO Two-component system sensor histidine kinase/response OS=Xanthomarina gelatinilytica OX=1137281 GN=D778_02690 PE=4 SV=1
MM1 pKa = 8.08 DD2 pKa = 5.78 LLRR5 pKa = 11.84 HH6 pKa = 5.76 VIIVTKK12 pKa = 10.47 NPNGSVCKK20 pKa = 10.35 NYY22 pKa = 10.48 NPNIRR27 pKa = 11.84 NHH29 pKa = 5.38 VKK31 pKa = 10.29 RR32 pKa = 11.84 NQKK35 pKa = 9.71 ILIRR39 pKa = 11.84 EE40 pKa = 4.11
Molecular weight: 4.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.736
IPC_protein 10.248
Toseland 10.818
ProMoST 10.394
Dawson 10.891
Bjellqvist 10.54
Wikipedia 11.052
Rodwell 11.286
Grimsley 10.921
Solomon 10.994
Lehninger 10.979
Nozaki 10.804
DTASelect 10.54
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.818
Patrickios 11.096
IPC_peptide 11.008
IPC2_peptide 9.472
IPC2.peptide.svr19 8.572
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2841
0
2841
902307
37
3221
317.6
35.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.337 ± 0.044
0.761 ± 0.018
5.497 ± 0.034
6.52 ± 0.051
5.266 ± 0.034
6.13 ± 0.054
1.967 ± 0.022
7.877 ± 0.038
7.815 ± 0.068
9.442 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.262 ± 0.029
6.346 ± 0.057
3.377 ± 0.026
3.578 ± 0.032
3.087 ± 0.033
6.331 ± 0.037
5.986 ± 0.052
6.28 ± 0.038
0.989 ± 0.018
4.151 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here