Halostagnicola larsenii XH-48

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Halostagnicola; Halostagnicola larsenii

Average proteome isoelectric point is 4.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4086 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0JSI0|W0JSI0_9EURY Sodium:solute symporter OS=Halostagnicola larsenii XH-48 OX=797299 GN=HALLA_16980 PE=3 SV=1
MM1 pKa = 7.43GALSGLSGSAAAQSSGGSQFGLDD24 pKa = 3.68AGFADD29 pKa = 5.13TSWLDD34 pKa = 3.49DD35 pKa = 4.28DD36 pKa = 5.63VDD38 pKa = 3.71VHH40 pKa = 6.73TITEE44 pKa = 4.21PTRR47 pKa = 11.84SAVEE51 pKa = 4.09DD52 pKa = 3.71AFSASGSRR60 pKa = 11.84VVVFEE65 pKa = 3.97TSGTIDD71 pKa = 3.81LGGNDD76 pKa = 3.99LSISEE81 pKa = 4.64DD82 pKa = 3.94YY83 pKa = 10.95CWVAGQTAPSPGITFINGQVQIDD106 pKa = 3.72ADD108 pKa = 3.89NCVVQHH114 pKa = 5.3IRR116 pKa = 11.84SRR118 pKa = 11.84IGPGSDD124 pKa = 4.29GSIQSNDD131 pKa = 3.4SFNTADD137 pKa = 3.47DD138 pKa = 3.92TQNNVVDD145 pKa = 4.49HH146 pKa = 6.64VSASWGTDD154 pKa = 2.56EE155 pKa = 4.91CLSVGYY161 pKa = 8.02DD162 pKa = 3.51TQDD165 pKa = 3.21TTVSNCLIYY174 pKa = 10.61EE175 pKa = 4.4GLYY178 pKa = 10.68DD179 pKa = 4.03PYY181 pKa = 11.4GDD183 pKa = 4.53EE184 pKa = 4.45SDD186 pKa = 4.34HH187 pKa = 7.17NYY189 pKa = 10.63GSLIGDD195 pKa = 4.09GASNVTLAGNVWAKK209 pKa = 10.16VRR211 pKa = 11.84GRR213 pKa = 11.84APRR216 pKa = 11.84LKK218 pKa = 10.39SDD220 pKa = 3.45TEE222 pKa = 4.21TAVVNNLLYY231 pKa = 10.89FFDD234 pKa = 4.18EE235 pKa = 4.43SANADD240 pKa = 3.47SSAVTSFVGNAAICADD256 pKa = 4.48DD257 pKa = 5.56DD258 pKa = 4.28DD259 pKa = 7.06AILEE263 pKa = 4.33GSPTAYY269 pKa = 10.31HH270 pKa = 7.33ADD272 pKa = 3.71NIAYY276 pKa = 7.95DD277 pKa = 4.11PPMEE281 pKa = 5.39DD282 pKa = 3.97DD283 pKa = 4.01QPIAEE288 pKa = 4.7PEE290 pKa = 4.57SVSSPPLWPSGLSEE304 pKa = 3.82MAANSVEE311 pKa = 4.05DD312 pKa = 4.21HH313 pKa = 6.45NLANAGARR321 pKa = 11.84PADD324 pKa = 4.04RR325 pKa = 11.84TAHH328 pKa = 5.9DD329 pKa = 3.4QRR331 pKa = 11.84IVQEE335 pKa = 3.79IADD338 pKa = 4.61RR339 pKa = 11.84DD340 pKa = 3.86GLDD343 pKa = 3.98YY344 pKa = 11.24LDD346 pKa = 4.62SPYY349 pKa = 10.66DD350 pKa = 3.69YY351 pKa = 10.64WVGAPDD357 pKa = 4.87DD358 pKa = 3.89VGGYY362 pKa = 9.51PDD364 pKa = 5.32LPVNTHH370 pKa = 5.38SLEE373 pKa = 4.27VPDD376 pKa = 3.8SDD378 pKa = 4.43IRR380 pKa = 11.84GWLAGWAQSVEE391 pKa = 4.13

Molecular weight: 41.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0JYJ7|W0JYJ7_9EURY Acyl-CoA dehydrogenase OS=Halostagnicola larsenii XH-48 OX=797299 GN=HALLA_01905 PE=3 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84PLIKK6 pKa = 10.13HH7 pKa = 6.0RR8 pKa = 11.84EE9 pKa = 3.81FRR11 pKa = 11.84PIDD14 pKa = 3.56HH15 pKa = 6.86AHH17 pKa = 6.44NARR20 pKa = 11.84IDD22 pKa = 3.49GTLYY26 pKa = 10.37GQSALSVTVFSVIKK40 pKa = 9.23RR41 pKa = 11.84TLGVAEE47 pKa = 4.94RR48 pKa = 11.84ARR50 pKa = 11.84SWYY53 pKa = 9.98RR54 pKa = 11.84EE55 pKa = 3.69LRR57 pKa = 11.84EE58 pKa = 4.06IVLMCAVYY66 pKa = 10.13NIKK69 pKa = 10.48RR70 pKa = 11.84AVSS73 pKa = 3.29

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4086

0

4086

1126012

29

1757

275.6

30.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.931 ± 0.05

0.743 ± 0.012

8.263 ± 0.05

9.229 ± 0.051

3.381 ± 0.033

8.313 ± 0.041

1.999 ± 0.018

4.844 ± 0.028

1.873 ± 0.021

8.743 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.841 ± 0.016

2.566 ± 0.022

4.528 ± 0.028

2.631 ± 0.022

6.147 ± 0.038

6.054 ± 0.033

6.576 ± 0.031

8.457 ± 0.036

1.13 ± 0.016

2.751 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski