Streptomyces bungoensis

Taxonomy: cellular organisms; Bacteria;

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6972 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A101T3T9|A0A101T3T9_9ACTN 50S ribosomal protein L6 OS=Streptomyces bungoensis OX=285568 GN=rplF PE=3 SV=1
MM1 pKa = 7.41TVQQEE6 pKa = 4.49APGGEE11 pKa = 4.07ALEE14 pKa = 4.15VWIDD18 pKa = 3.41QDD20 pKa = 3.99LCTGDD25 pKa = 5.01GICAQYY31 pKa = 10.9APEE34 pKa = 4.23VFEE37 pKa = 5.9LDD39 pKa = 3.05IDD41 pKa = 3.65GLAYY45 pKa = 10.7VKK47 pKa = 10.83GADD50 pKa = 5.48DD51 pKa = 4.28EE52 pKa = 5.12LLQNRR57 pKa = 11.84GATVPVPLPLLADD70 pKa = 3.69VVDD73 pKa = 4.35SAKK76 pKa = 10.23EE77 pKa = 3.93CPGEE81 pKa = 4.37CIHH84 pKa = 6.56VRR86 pKa = 11.84RR87 pKa = 11.84AEE89 pKa = 4.47DD90 pKa = 3.53GVEE93 pKa = 4.28VYY95 pKa = 10.9GPDD98 pKa = 3.7AEE100 pKa = 4.31

Molecular weight: 10.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A101TC95|A0A101TC95_9ACTN CDP-diacylglycerol--serine O-phosphatidyltransferase OS=Streptomyces bungoensis OX=285568 GN=AQJ66_03560 PE=3 SV=1
MM1 pKa = 7.4VGVGAGRR8 pKa = 11.84WSRR11 pKa = 11.84MLLGGLAGGFANGVTAGLVLGGFAAGLVPRR41 pKa = 11.84GFAAGLVPGRR51 pKa = 11.84LTTGLIPRR59 pKa = 11.84GLPARR64 pKa = 11.84LVPGRR69 pKa = 11.84LTTRR73 pKa = 11.84LIPRR77 pKa = 11.84GLPP80 pKa = 3.23

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6972

0

6972

2307537

29

6659

331.0

35.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.929 ± 0.051

0.795 ± 0.007

5.955 ± 0.024

5.582 ± 0.029

2.676 ± 0.016

9.574 ± 0.03

2.417 ± 0.016

2.89 ± 0.021

2.026 ± 0.025

10.509 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.646 ± 0.013

1.648 ± 0.016

6.17 ± 0.029

2.721 ± 0.018

8.385 ± 0.033

4.846 ± 0.021

6.107 ± 0.023

8.526 ± 0.024

1.511 ± 0.012

2.087 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski