Streptomyces curacoi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7114 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A124GXP9|A0A124GXP9_9ACTN GTP pyrophosphokinase OS=Streptomyces curacoi OX=146536 GN=AQI70_26530 PE=3 SV=1
MM1 pKa = 7.1QSTYY5 pKa = 10.51IAGQEE10 pKa = 4.2VVLDD14 pKa = 4.07DD15 pKa = 4.64APIFGPVDD23 pKa = 3.24VDD25 pKa = 3.82DD26 pKa = 4.62GVVVIVQEE34 pKa = 4.25LGPVRR39 pKa = 11.84GFSAAEE45 pKa = 3.56VWGCLVSDD53 pKa = 4.25HH54 pKa = 6.65LCWHH58 pKa = 7.14AA59 pKa = 4.6

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A117NUT8|A0A117NUT8_9ACTN Alpha-L-fucosidase OS=Streptomyces curacoi OX=146536 GN=AQI70_35425 PE=4 SV=1
MM1 pKa = 7.35AVVPVAAVVPVAVAVVRR18 pKa = 11.84AAAVSPVVPAVPVAVAAVSPVVPAVPVAVAAVSPAVLVVPAVAAVAVPASVVVPAVPVAVVARR81 pKa = 11.84RR82 pKa = 11.84VPSAVPAVPRR92 pKa = 11.84VVAVSRR98 pKa = 11.84SGRR101 pKa = 11.84GARR104 pKa = 11.84STRR107 pKa = 11.84PCRR110 pKa = 11.84PRR112 pKa = 11.84RR113 pKa = 11.84SAAA116 pKa = 3.18

Molecular weight: 11.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7114

0

7114

2369746

29

4557

333.1

35.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.371 ± 0.048

0.793 ± 0.009

6.009 ± 0.025

5.846 ± 0.031

2.749 ± 0.018

9.344 ± 0.03

2.298 ± 0.014

3.211 ± 0.021

2.32 ± 0.027

10.296 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.732 ± 0.013

1.828 ± 0.019

6.04 ± 0.024

2.817 ± 0.017

7.969 ± 0.034

5.021 ± 0.023

6.128 ± 0.026

8.483 ± 0.029

1.559 ± 0.012

2.187 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski