Microvirga ossetica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Microvirga

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8355 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2EUZ9|A0A1B2EUZ9_9RHIZ Uncharacterized protein OS=Microvirga ossetica OX=1882682 GN=BB934_36735 PE=4 SV=1
MM1 pKa = 7.32RR2 pKa = 11.84TGLNAHH8 pKa = 7.03LAALAVATALGTAAHH23 pKa = 6.67AADD26 pKa = 4.45LPSRR30 pKa = 11.84FAPPPVAAAVPVFTWTGFYY49 pKa = 10.78VGVNAGYY56 pKa = 10.27GWNTNDD62 pKa = 4.69DD63 pKa = 3.92DD64 pKa = 4.71VVINGTAFEE73 pKa = 4.28VDD75 pKa = 3.6DD76 pKa = 4.36EE77 pKa = 4.93GGFVGGAQIGYY88 pKa = 9.15NYY90 pKa = 10.05QIGSFVVGLEE100 pKa = 3.75TDD102 pKa = 2.86IQYY105 pKa = 11.58ADD107 pKa = 3.37IGGDD111 pKa = 3.39TTLPGLDD118 pKa = 4.04SHH120 pKa = 7.88DD121 pKa = 5.88DD122 pKa = 3.54DD123 pKa = 6.28DD124 pKa = 4.1NWFGTVRR131 pKa = 11.84GRR133 pKa = 11.84AGYY136 pKa = 10.72AFDD139 pKa = 4.32RR140 pKa = 11.84ALIYY144 pKa = 9.82ATGGLAYY151 pKa = 10.16GKK153 pKa = 10.01ISNGFSNSDD162 pKa = 3.4DD163 pKa = 4.0TNVGWTLGAGVEE175 pKa = 4.3YY176 pKa = 10.8AFTNNLTAKK185 pKa = 10.68VEE187 pKa = 4.04GLYY190 pKa = 11.2VNLEE194 pKa = 3.84QDD196 pKa = 4.33DD197 pKa = 4.68DD198 pKa = 4.3DD199 pKa = 4.52VPNISGKK206 pKa = 10.44DD207 pKa = 3.21EE208 pKa = 4.3TEE210 pKa = 3.97FGVIRR215 pKa = 11.84AGLNYY220 pKa = 10.55KK221 pKa = 8.81FTTYY225 pKa = 11.08

Molecular weight: 23.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B2EIT7|A0A1B2EIT7_9RHIZ DUF58 domain-containing protein OS=Microvirga ossetica OX=1882682 GN=BB934_18050 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38GGRR28 pKa = 11.84KK29 pKa = 9.21VIAARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.11GRR39 pKa = 11.84KK40 pKa = 9.3RR41 pKa = 11.84LSAA44 pKa = 4.01

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8355

0

8355

2404899

29

3418

287.8

31.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.814 ± 0.031

0.828 ± 0.009

5.563 ± 0.023

5.811 ± 0.025

3.654 ± 0.019

8.357 ± 0.036

2.113 ± 0.014

5.242 ± 0.02

3.306 ± 0.022

10.221 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.363 ± 0.012

2.585 ± 0.019

5.267 ± 0.021

3.326 ± 0.016

7.443 ± 0.032

5.634 ± 0.021

5.372 ± 0.02

7.481 ± 0.023

1.389 ± 0.012

2.232 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski