Granulicella pectinivorans
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4259 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I6L8V8|A0A1I6L8V8_9BACT Quinolinate phosphoribosyltransferase [decarboxylating] OS=Granulicella pectinivorans OX=474950 GN=SAMN05421771_0428 PE=3 SV=1
MM1 pKa = 7.51 PEE3 pKa = 4.05 EE4 pKa = 4.21 VRR6 pKa = 11.84 STTANQLVILFEE18 pKa = 4.37 GSWFFIQDD26 pKa = 3.68 PEE28 pKa = 4.46 DD29 pKa = 3.39 STRR32 pKa = 11.84 ILALCPYY39 pKa = 9.83 VDD41 pKa = 5.48 APDD44 pKa = 3.72 HH45 pKa = 5.78 VCWFGFWDD53 pKa = 4.68 GSSIAGPNATGPNGAMQEE71 pKa = 4.4 GEE73 pKa = 4.61 SLWVDD78 pKa = 4.16 VITDD82 pKa = 3.58 QAKK85 pKa = 8.38 NTAPVDD91 pKa = 3.75 ALFQAAQAAYY101 pKa = 9.58 SFPYY105 pKa = 8.98 LTSSDD110 pKa = 4.01 PATSPLQLTDD120 pKa = 3.28 TTGMRR125 pKa = 11.84 RR126 pKa = 11.84 VSISMPDD133 pKa = 3.16 SMRR136 pKa = 11.84 ADD138 pKa = 3.34 GLLTNATVYY147 pKa = 10.75 GSQNPGSTIKK157 pKa = 10.72 LDD159 pKa = 3.73 AGSSYY164 pKa = 11.5 VDD166 pKa = 3.73 FLFVYY171 pKa = 10.66 YY172 pKa = 10.55 YY173 pKa = 8.81 DD174 pKa = 3.88 TQADD178 pKa = 3.72 VAMGTQFFEE187 pKa = 4.35 QPVFSTSLATVVPHH201 pKa = 7.24 LIFKK205 pKa = 10.75 VMGTAADD212 pKa = 3.91 AQDD215 pKa = 3.3 MASEE219 pKa = 4.42 YY220 pKa = 9.35 THH222 pKa = 7.11 LVQTFDD228 pKa = 3.88 SLRR231 pKa = 11.84 QMVFSPDD238 pKa = 4.39 GICCDD243 pKa = 3.31 VAIYY247 pKa = 10.28 PEE249 pKa = 4.62 AGQRR253 pKa = 11.84 LQFEE257 pKa = 4.37 ILDD260 pKa = 3.96 PSFSAAEE267 pKa = 4.05 LGIPSQQLGGLRR279 pKa = 11.84 IRR281 pKa = 11.84 DD282 pKa = 3.91 YY283 pKa = 11.15 ASCAAGPFAASGVLGGG299 pKa = 4.3
Molecular weight: 32.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.923
IPC_protein 3.935
Toseland 3.706
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.872
Rodwell 3.757
Grimsley 3.617
Solomon 3.923
Lehninger 3.872
Nozaki 4.037
DTASelect 4.304
Thurlkill 3.77
EMBOSS 3.884
Sillero 4.05
Patrickios 0.731
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.913
Protein with the highest isoelectric point:
>tr|A0A1I6LHL1|A0A1I6LHL1_9BACT Uncharacterized protein OS=Granulicella pectinivorans OX=474950 GN=SAMN05421771_0711 PE=4 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.02 RR4 pKa = 11.84 TFQPNRR10 pKa = 11.84 RR11 pKa = 11.84 HH12 pKa = 5.69 RR13 pKa = 11.84 AKK15 pKa = 9.55 THH17 pKa = 5.47 GFLTRR22 pKa = 11.84 MKK24 pKa = 9.03 TKK26 pKa = 10.57 AGAAVLSRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.19 GRR40 pKa = 11.84 HH41 pKa = 5.72 KK42 pKa = 10.29 IAVSAGFRR50 pKa = 11.84 DD51 pKa = 3.66
Molecular weight: 5.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.403
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4259
0
4259
1581086
29
2642
371.2
40.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.182 ± 0.041
0.831 ± 0.011
4.992 ± 0.029
5.155 ± 0.047
3.918 ± 0.026
8.248 ± 0.045
2.267 ± 0.019
4.966 ± 0.03
3.691 ± 0.032
9.989 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.38 ± 0.019
3.274 ± 0.036
5.456 ± 0.026
3.645 ± 0.029
5.867 ± 0.043
6.121 ± 0.039
6.562 ± 0.056
7.363 ± 0.031
1.325 ± 0.016
2.768 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here