Anaeromicrobium sediminis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Anaeromicrobium

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4258 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A267MKM4|A0A267MKM4_9CLOT Pyridoxamine 5'-phosphate oxidase OS=Anaeromicrobium sediminis OX=1478221 GN=CCE28_07155 PE=4 SV=1
MM1 pKa = 7.13MSAMYY6 pKa = 10.3SGVSGMQAHH15 pKa = 5.17QVKK18 pKa = 10.64LDD20 pKa = 3.79VISNNIANINTVGYY34 pKa = 9.66KK35 pKa = 10.06AQTVSFEE42 pKa = 4.18EE43 pKa = 5.4VYY45 pKa = 10.99SQTLNGATAPSDD57 pKa = 3.5SGRR60 pKa = 11.84GGTNPMQLGHH70 pKa = 6.48GVTVSSINTINTQGSMQLTGGTTDD94 pKa = 3.98FAIDD98 pKa = 3.32GDD100 pKa = 4.54GYY102 pKa = 10.97FIVQDD107 pKa = 3.63PSGAYY112 pKa = 8.61MFTRR116 pKa = 11.84AGNFGLDD123 pKa = 3.12TDD125 pKa = 4.66GNLVTADD132 pKa = 3.5GLYY135 pKa = 8.86VCGWQEE141 pKa = 4.34YY142 pKa = 8.77EE143 pKa = 4.22ALPDD147 pKa = 3.37GTYY150 pKa = 10.98VFDD153 pKa = 3.7TTKK156 pKa = 10.85DD157 pKa = 3.54PEE159 pKa = 4.99PINIFSDD166 pKa = 3.96EE167 pKa = 4.11YY168 pKa = 11.58NKK170 pKa = 10.73NKK172 pKa = 10.53NNIAPKK178 pKa = 8.48ATEE181 pKa = 3.78NVVLNGNLDD190 pKa = 3.43ASEE193 pKa = 4.31SPKK196 pKa = 10.87GSGTSDD202 pKa = 2.4IGTPPEE208 pKa = 4.04TADD211 pKa = 3.11FTMPFTAYY219 pKa = 10.43DD220 pKa = 3.74DD221 pKa = 4.47LGNDD225 pKa = 3.48YY226 pKa = 10.93EE227 pKa = 4.61VDD229 pKa = 3.81VNFTKK234 pKa = 10.64CYY236 pKa = 10.7VDD238 pKa = 6.13DD239 pKa = 4.96SDD241 pKa = 4.77PDD243 pKa = 3.74NPVTTWYY250 pKa = 9.96WEE252 pKa = 4.13MPSDD256 pKa = 4.3GSSSSSGYY264 pKa = 10.36IKK266 pKa = 10.32FDD268 pKa = 3.2KK269 pKa = 10.7DD270 pKa = 3.47GNIVEE275 pKa = 4.64EE276 pKa = 5.03DD277 pKa = 3.69GFDD280 pKa = 3.27TKK282 pKa = 11.21VDD284 pKa = 3.76VEE286 pKa = 4.38VTPDD290 pKa = 3.06SSTGTGSFTMTIDD303 pKa = 3.85FEE305 pKa = 4.78SVSMYY310 pKa = 10.62DD311 pKa = 3.62AEE313 pKa = 5.07SSVNPLNVDD322 pKa = 4.24GYY324 pKa = 10.36PSAEE328 pKa = 4.19LNDD331 pKa = 4.38FYY333 pKa = 10.96IGADD337 pKa = 3.6GVIMGVYY344 pKa = 10.42SNGQQQPLGMMSLATFSNPAGLEE367 pKa = 3.78RR368 pKa = 11.84VGNNLFMATANSGDD382 pKa = 3.69FNQAYY387 pKa = 10.31APGTQGAGTLSVGFLEE403 pKa = 4.5MSNVDD408 pKa = 3.63LSSEE412 pKa = 4.0FTEE415 pKa = 4.07MVIAQRR421 pKa = 11.84GFQANSRR428 pKa = 11.84IITTADD434 pKa = 2.97EE435 pKa = 4.78MIQEE439 pKa = 5.12LINLKK444 pKa = 10.3RR445 pKa = 3.56

Molecular weight: 47.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A267MF73|A0A267MF73_9CLOT Uncharacterized protein OS=Anaeromicrobium sediminis OX=1478221 GN=CCE28_16485 PE=3 SV=1
MM1 pKa = 7.35ARR3 pKa = 11.84KK4 pKa = 9.59ALVVKK9 pKa = 7.44QQRR12 pKa = 11.84KK13 pKa = 6.5QKK15 pKa = 10.41YY16 pKa = 6.11STRR19 pKa = 11.84EE20 pKa = 3.65YY21 pKa = 10.59NRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 6.75AYY33 pKa = 9.43LRR35 pKa = 11.84KK36 pKa = 9.69FGICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.81LAYY49 pKa = 10.0KK50 pKa = 10.86GEE52 pKa = 4.16IPGVRR57 pKa = 11.84KK58 pKa = 10.13ASWW61 pKa = 2.78

Molecular weight: 7.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4258

0

4258

1330799

26

2067

312.5

35.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.469 ± 0.043

1.121 ± 0.017

5.521 ± 0.03

7.715 ± 0.046

4.258 ± 0.031

6.724 ± 0.04

1.654 ± 0.016

9.914 ± 0.044

8.972 ± 0.039

8.961 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.926 ± 0.016

5.859 ± 0.036

2.931 ± 0.023

2.205 ± 0.019

3.498 ± 0.028

5.931 ± 0.031

4.872 ± 0.026

6.708 ± 0.035

0.713 ± 0.011

4.047 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski