Longimonas halophila

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidetes Order II. Incertae sedis; Rhodothermaceae; Longimonas

Average proteome isoelectric point is 5.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2920 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H3NR13|A0A2H3NR13_9BACT DUF547 domain-containing protein OS=Longimonas halophila OX=1469170 GN=CRI93_11595 PE=4 SV=1
MM1 pKa = 7.18PHH3 pKa = 6.97SVMTRR8 pKa = 11.84VFCTAALALLCMLALPTTADD28 pKa = 3.35AQVGFTVGPRR38 pKa = 11.84LSLDD42 pKa = 3.01IGDD45 pKa = 4.67DD46 pKa = 3.76LEE48 pKa = 4.73EE49 pKa = 5.08GSTTFAAGADD59 pKa = 3.68VRR61 pKa = 11.84ISSVALPFIINPTYY75 pKa = 11.05DD76 pKa = 4.06FYY78 pKa = 11.64FLDD81 pKa = 4.93DD82 pKa = 4.34EE83 pKa = 4.86NGADD87 pKa = 4.24INFSTLNVNALFPFGVSNQVFTPYY111 pKa = 10.05TGVGLGFSFLAQGPADD127 pKa = 3.79TVTDD131 pKa = 3.67AGINIVTGAQFDD143 pKa = 4.56LLPLKK148 pKa = 10.56PFVEE152 pKa = 4.72LGYY155 pKa = 10.49SVYY158 pKa = 9.44FTDD161 pKa = 5.81GDD163 pKa = 3.75QNLNAFGIRR172 pKa = 11.84GGLLFGLL179 pKa = 4.67

Molecular weight: 18.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H3NPK3|A0A2H3NPK3_9BACT DUF4835 domain-containing protein OS=Longimonas halophila OX=1469170 GN=CRI93_07880 PE=4 SV=1
MM1 pKa = 7.5AVPKK5 pKa = 10.1RR6 pKa = 11.84RR7 pKa = 11.84HH8 pKa = 5.23SKK10 pKa = 9.22SRR12 pKa = 11.84SRR14 pKa = 11.84KK15 pKa = 8.55RR16 pKa = 11.84RR17 pKa = 11.84SRR19 pKa = 11.84YY20 pKa = 9.29YY21 pKa = 10.78NSLKK25 pKa = 10.58APQLMEE31 pKa = 4.98CNNCGNPKK39 pKa = 9.84VMHH42 pKa = 6.71RR43 pKa = 11.84ACKK46 pKa = 9.92FCGHH50 pKa = 5.1YY51 pKa = 9.85RR52 pKa = 11.84GRR54 pKa = 11.84QVVEE58 pKa = 3.91PTEE61 pKa = 3.99EE62 pKa = 3.97LLGG65 pKa = 4.01

Molecular weight: 7.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2920

0

2920

1046801

16

2848

358.5

39.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.793 ± 0.063

0.699 ± 0.013

6.943 ± 0.042

6.401 ± 0.052

3.475 ± 0.03

7.578 ± 0.055

2.494 ± 0.035

4.419 ± 0.035

2.079 ± 0.033

9.452 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.186 ± 0.024

3.02 ± 0.035

5.385 ± 0.032

3.933 ± 0.03

6.806 ± 0.05

5.933 ± 0.04

6.693 ± 0.041

7.466 ± 0.039

1.369 ± 0.018

2.876 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski