Hydrogenibacillus schlegelii (Bacillus schlegelii)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Hydrogenibacillus

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2254 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A179IQR0|A0A179IQR0_HYDSH Uncharacterized protein OS=Hydrogenibacillus schlegelii OX=1484 GN=SA87_06590 PE=4 SV=1
MM1 pKa = 7.7PNYY4 pKa = 10.58SVFQANPEE12 pKa = 4.02EE13 pKa = 4.22LKK15 pKa = 11.2ALIFGSDD22 pKa = 3.17GTTARR27 pKa = 11.84PLAVNASAEE36 pKa = 4.16LLVGGATVTGGTLDD50 pKa = 3.52AVSAATIAGGTLDD63 pKa = 4.16AVSAATIAGGTLDD76 pKa = 4.16AVSAATIAGGTLDD89 pKa = 4.16AVSAATIAGGTLDD102 pKa = 4.16AVSAATIAGGTLDD115 pKa = 4.16AVSAATIAGGTLDD128 pKa = 3.82SVTSISQRR136 pKa = 11.84SFLEE140 pKa = 3.73IANTDD145 pKa = 3.38VATGDD150 pKa = 3.7TLTALPAVTTAVLGHH165 pKa = 5.98YY166 pKa = 10.5SYY168 pKa = 11.24FIYY171 pKa = 10.76NAGANDD177 pKa = 3.3AVAQVEE183 pKa = 4.21ISADD187 pKa = 3.45GTHH190 pKa = 7.38WYY192 pKa = 9.2TDD194 pKa = 3.28IPSTTVASGSVAVLVPTRR212 pKa = 11.84FLKK215 pKa = 9.05YY216 pKa = 8.96TRR218 pKa = 11.84LAYY221 pKa = 10.32ASAVVGAATTIDD233 pKa = 3.72VYY235 pKa = 11.38FNAQGTT241 pKa = 3.9

Molecular weight: 23.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A179IPZ7|A0A179IPZ7_HYDSH Uncharacterized protein OS=Hydrogenibacillus schlegelii OX=1484 GN=SA87_09630 PE=4 SV=1
MM1 pKa = 7.82PSFVLCPSIFPARR14 pKa = 11.84LTTPAGPSFAPRR26 pKa = 11.84RR27 pKa = 11.84SVFLLPTGGVKK38 pKa = 9.76RR39 pKa = 11.84VVRR42 pKa = 11.84NGRR45 pKa = 11.84RR46 pKa = 11.84AARR49 pKa = 11.84LLFPGGRR56 pKa = 11.84SGHH59 pKa = 5.61RR60 pKa = 3.21

Molecular weight: 6.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2254

0

2254

688065

37

1680

305.3

33.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.1 ± 0.102

0.399 ± 0.012

4.958 ± 0.041

6.944 ± 0.061

3.795 ± 0.032

8.926 ± 0.058

1.828 ± 0.024

4.535 ± 0.043

3.09 ± 0.04

10.836 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.939 ± 0.023

1.786 ± 0.025

5.924 ± 0.046

2.154 ± 0.028

9.004 ± 0.059

4.075 ± 0.029

4.605 ± 0.036

8.335 ± 0.045

1.289 ± 0.021

2.477 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski