Bacillus taxi
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5402 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S2TV05|A0A3S2TV05_9BACI Malate dehydrogenase OS=Bacillus taxi OX=2499688 GN=mdh PE=3 SV=1
MM1 pKa = 7.94 PIALQAFTNTANTVRR16 pKa = 11.84 IADD19 pKa = 3.84 SATAPGPVVTVGPQIPLVDD38 pKa = 4.25 GNSSYY43 pKa = 10.38 IWSPVSVSGQTVTFRR58 pKa = 11.84 SVFSLGAPLLGIALPLTVFYY78 pKa = 10.4 AYY80 pKa = 10.37 AGNEE84 pKa = 4.35 TVSVTGTLQVLDD96 pKa = 3.77 VLGVIVLTIPLFSGDD111 pKa = 3.84 TNLGNPLNVSTIAADD126 pKa = 3.71 TLLAASILGGTINITIDD143 pKa = 3.44 AVVTAPITTPYY154 pKa = 10.57 EE155 pKa = 3.85 PVNTGRR161 pKa = 11.84 YY162 pKa = 8.69 LGEE165 pKa = 3.84 ITVQTIVV172 pKa = 3.09
Molecular weight: 17.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.884
IPC_protein 3.745
Toseland 3.554
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.681
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.884
Patrickios 0.604
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|A0A3S2X3A1|A0A3S2X3A1_9BACI Alcohol dehydrogenase OS=Bacillus taxi OX=2499688 GN=EM808_12390 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPNKK9 pKa = 8.24 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 SKK14 pKa = 9.59 VHH16 pKa = 5.83 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MSSPNGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.05 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5402
0
5402
1569371
26
2055
290.5
32.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.097 ± 0.037
0.709 ± 0.01
5.085 ± 0.032
7.342 ± 0.038
4.579 ± 0.029
6.691 ± 0.032
1.944 ± 0.017
8.193 ± 0.03
7.237 ± 0.033
9.867 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.714 ± 0.017
4.765 ± 0.028
3.351 ± 0.02
3.553 ± 0.021
3.61 ± 0.025
6.337 ± 0.027
5.472 ± 0.027
6.725 ± 0.029
1.038 ± 0.012
3.692 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here