Sanxia Water Strider Virus 2
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L4AB05|A0A1L4AB05_9VIRU Nucleocapsid protein OS=Sanxia Water Strider Virus 2 OX=1608061 GN=N PE=4 SV=1
MM1 pKa = 7.53 ALIIVLTLFAALSNAEE17 pKa = 4.26 YY18 pKa = 10.7 LRR20 pKa = 11.84 EE21 pKa = 4.03 TYY23 pKa = 10.3 DD24 pKa = 2.67 WYY26 pKa = 9.94 TAGKK30 pKa = 9.7 NNDD33 pKa = 2.7 ISGLHH38 pKa = 6.05 NLIQKK43 pKa = 8.51 EE44 pKa = 4.24 PKK46 pKa = 9.91 NMEE49 pKa = 4.1 IKK51 pKa = 10.5 SEE53 pKa = 4.01 SCEE56 pKa = 3.93 NGGCRR61 pKa = 11.84 LSLEE65 pKa = 4.32 RR66 pKa = 11.84 NFNILTSKK74 pKa = 10.76 GFGINFYY81 pKa = 9.82 VDD83 pKa = 2.85 EE84 pKa = 4.46 MGYY87 pKa = 10.58 VNNTILVQVMDD98 pKa = 3.55 TTFNVTAKK106 pKa = 10.56 YY107 pKa = 10.47 KK108 pKa = 9.62 YY109 pKa = 6.19 TTPACKK115 pKa = 10.12 YY116 pKa = 8.68 FYY118 pKa = 10.9 RR119 pKa = 11.84 NAFFNYY125 pKa = 9.09 NQQKK129 pKa = 9.6 YY130 pKa = 9.29 ANCKK134 pKa = 9.97 SGLGTDD140 pKa = 4.01 DD141 pKa = 3.8 FMSIAEE147 pKa = 4.06 MQAYY151 pKa = 9.14 GQFGVDD157 pKa = 3.36 IEE159 pKa = 4.49 VADD162 pKa = 5.07 RR163 pKa = 11.84 KK164 pKa = 8.79 PTLLDD169 pKa = 3.5 LGGWSEE175 pKa = 3.96 EE176 pKa = 4.32 AGLCGHH182 pKa = 7.35 WWPTVLKK189 pKa = 9.48 TCFEE193 pKa = 4.2 KK194 pKa = 11.14 ALICSLKK201 pKa = 10.46 EE202 pKa = 4.05 SFVLFALEE210 pKa = 3.92 PEE212 pKa = 4.65 IVFEE216 pKa = 3.45 MWIRR220 pKa = 11.84 VTVGQNTYY228 pKa = 11.17 DD229 pKa = 3.36 VVYY232 pKa = 10.37 EE233 pKa = 4.22 GNTNEE238 pKa = 3.71 MRR240 pKa = 11.84 TIEE243 pKa = 4.17 VSEE246 pKa = 4.72 GIYY249 pKa = 10.6 LDD251 pKa = 3.79 INPIGQLVNPINSKK265 pKa = 10.93 AIGAKK270 pKa = 8.97 IHH272 pKa = 5.69 YY273 pKa = 7.31 EE274 pKa = 3.95 KK275 pKa = 11.03 SNKK278 pKa = 6.77 TAKK281 pKa = 10.66 LEE283 pKa = 4.31 SQWVYY288 pKa = 11.03 LDD290 pKa = 4.31 SYY292 pKa = 9.53 PDD294 pKa = 3.15 SGQIVSQGVGKK305 pKa = 9.57 FQLVNLNNIKK315 pKa = 9.2 STISNKK321 pKa = 10.02 NNLNCDD327 pKa = 3.41 GTIQSCTKK335 pKa = 10.0 NLNRR339 pKa = 11.84 KK340 pKa = 8.72 LRR342 pKa = 11.84 TKK344 pKa = 8.68 QTNTGFSEE352 pKa = 4.27 KK353 pKa = 10.1 KK354 pKa = 10.49 LLDD357 pKa = 3.29 IYY359 pKa = 10.56 SIKK362 pKa = 10.98 NSDD365 pKa = 3.51 TVEE368 pKa = 4.61 DD369 pKa = 4.5 DD370 pKa = 5.14 LIIIAPNSSLQNLKK384 pKa = 11.01 GGDD387 pKa = 3.59 TCCIKK392 pKa = 10.7 LISPSEE398 pKa = 4.04 RR399 pKa = 11.84 FLDD402 pKa = 4.08 SIVNNYY408 pKa = 9.46 EE409 pKa = 3.68 EE410 pKa = 4.32 ANKK413 pKa = 10.21 VLMTKK418 pKa = 9.51 MPILPSSFKK427 pKa = 10.4 ISVSGTPEE435 pKa = 3.83 LDD437 pKa = 3.19 SFHH440 pKa = 7.02 FKK442 pKa = 10.94 NLLSVPILATLRR454 pKa = 11.84 TKK456 pKa = 10.91 DD457 pKa = 3.4 LFLPFSNKK465 pKa = 8.44 EE466 pKa = 3.82 FEE468 pKa = 4.13 ITNLIISSMTYY479 pKa = 10.06 FEE481 pKa = 4.34 NAAGSFFEE489 pKa = 4.3 MSFDD493 pKa = 3.72 YY494 pKa = 10.9 SGPPGNIRR502 pKa = 11.84 IDD504 pKa = 3.61 SKK506 pKa = 11.34 DD507 pKa = 3.21 INILTKK513 pKa = 10.06 EE514 pKa = 4.31 LYY516 pKa = 9.72 ISSSGKK522 pKa = 8.67 YY523 pKa = 8.11 TDD525 pKa = 3.39 TVGFKK530 pKa = 10.72 SKK532 pKa = 11.09 GIMALKK538 pKa = 9.03 PTICVGDD545 pKa = 3.66 AAHH548 pKa = 6.47 CVKK551 pKa = 9.84 PAKK554 pKa = 10.17 IINTKK559 pKa = 9.34 TKK561 pKa = 10.31 DD562 pKa = 3.61 GSVDD566 pKa = 3.44 DD567 pKa = 4.6 PMTGEE572 pKa = 4.25 EE573 pKa = 4.8 DD574 pKa = 3.65 SLINQITTMFKK585 pKa = 10.77 LYY587 pKa = 9.95 IANIWFCIFMTIIWLFDD604 pKa = 3.3 IVLSAVLIIIIVKK617 pKa = 9.77 LCGRR621 pKa = 11.84 STLMLTVLCCSKK633 pKa = 10.34 RR634 pKa = 11.84 GEE636 pKa = 4.34 SIEE639 pKa = 4.01 TSKK642 pKa = 10.44 ICEE645 pKa = 4.19 LEE647 pKa = 3.98 KK648 pKa = 11.12 VGTLSSIIRR657 pKa = 11.84 FDD659 pKa = 4.18 HH660 pKa = 5.67 IVGVKK665 pKa = 10.03 LEE667 pKa = 4.1 KK668 pKa = 11.13 SMTDD672 pKa = 3.69 LSWMINDD679 pKa = 3.42 QMKK682 pKa = 10.35 VLSINMPTKK691 pKa = 10.69 SSLLEE696 pKa = 3.78 PKK698 pKa = 10.07 VKK700 pKa = 10.39 NFYY703 pKa = 10.5 HH704 pKa = 6.45 VGVANYY710 pKa = 9.14 SHH712 pKa = 7.43 EE713 pKa = 4.85 DD714 pKa = 3.47 GAHH717 pKa = 6.41 FLLPLYY723 pKa = 9.87 GANPWEE729 pKa = 5.18 IGTCDD734 pKa = 5.12 DD735 pKa = 5.45 DD736 pKa = 6.41 NDD738 pKa = 4.7 DD739 pKa = 4.04 SGCNLEE745 pKa = 5.56 EE746 pKa = 3.93 ITADD750 pKa = 3.05 TVTRR754 pKa = 11.84 NTNFLLNLWNQVPRR768 pKa = 11.84 VTQFLYY774 pKa = 10.27 IKK776 pKa = 8.27 STSYY780 pKa = 10.24 PRR782 pKa = 11.84 HH783 pKa = 6.33 HH784 pKa = 7.26 YY785 pKa = 11.08 DD786 pKa = 3.67 NGCSTYY792 pKa = 10.2 TVALISASGTSSYY805 pKa = 10.56 ATILAYY811 pKa = 9.42 STAYY815 pKa = 9.53 TGINLIEE822 pKa = 3.9 VSKK825 pKa = 10.83 FGNSKK830 pKa = 9.22 IFAVEE835 pKa = 3.99 VVAVASYY842 pKa = 11.0 KK843 pKa = 10.37 MNIDD847 pKa = 3.39 VVKK850 pKa = 10.89 NNVRR854 pKa = 11.84 SMIKK858 pKa = 9.83 ADD860 pKa = 3.83 EE861 pKa = 4.23 NVVISPINKK870 pKa = 9.17 QSIDD874 pKa = 3.73 AIGRR878 pKa = 11.84 FYY880 pKa = 11.18 FLITCPSGCVIYY892 pKa = 10.99 NPLVNKK898 pKa = 10.1 NGKK901 pKa = 10.04 AEE903 pKa = 4.18 MIKK906 pKa = 8.94 ITDD909 pKa = 3.86 SLLKK913 pKa = 9.76 HH914 pKa = 6.13 QIYY917 pKa = 10.81 NDD919 pKa = 3.25 VSVFRR924 pKa = 11.84 SFWKK928 pKa = 8.96 TNKK931 pKa = 9.59 VNILSQEE938 pKa = 3.95 NKK940 pKa = 9.53 LSKK943 pKa = 10.64 DD944 pKa = 2.95 IVIRR948 pKa = 11.84 YY949 pKa = 8.43 QNDD952 pKa = 3.28 YY953 pKa = 10.5 NKK955 pKa = 10.57 KK956 pKa = 10.31 FGLDD960 pKa = 3.48 DD961 pKa = 3.64 VKK963 pKa = 11.47 SDD965 pKa = 3.58 VEE967 pKa = 4.41 SFINNKK973 pKa = 7.09 WYY975 pKa = 10.35 RR976 pKa = 11.84 YY977 pKa = 8.08 NLQKK981 pKa = 10.66 ISDD984 pKa = 4.06 NTDD987 pKa = 3.03 SNGDD991 pKa = 3.02 ITLDD995 pKa = 4.19 DD996 pKa = 4.15 NQLHH1000 pKa = 5.36 QLIDD1004 pKa = 3.63 PTSDD1008 pKa = 4.33 FIMTDD1013 pKa = 3.3 DD1014 pKa = 5.52 GILDD1018 pKa = 3.59 VTLNGPISMNCHH1030 pKa = 4.66 STGVGARR1037 pKa = 11.84 CRR1039 pKa = 11.84 HH1040 pKa = 4.86 EE1041 pKa = 4.25 TGNMIAHH1048 pKa = 7.31 FYY1050 pKa = 8.01 NTRR1053 pKa = 11.84 TDD1055 pKa = 3.08 INKK1058 pKa = 9.93 FGSFKK1063 pKa = 11.26 DD1064 pKa = 3.26 NGQSYY1069 pKa = 10.07 KK1070 pKa = 10.46 YY1071 pKa = 9.48 EE1072 pKa = 4.14 KK1073 pKa = 10.18 SDD1075 pKa = 3.63 YY1076 pKa = 10.74 KK1077 pKa = 11.27 VGLLPRR1083 pKa = 11.84 KK1084 pKa = 8.96 EE1085 pKa = 3.95 PCKK1088 pKa = 10.31 GKK1090 pKa = 10.86 GYY1092 pKa = 9.98 IDD1094 pKa = 3.45 SQSRR1098 pKa = 11.84 FLCSHH1103 pKa = 7.13 DD1104 pKa = 3.36 CCMVSTNNWNNYY1116 pKa = 9.03 FLVSANKK1123 pKa = 9.54 PFVPNRR1129 pKa = 11.84 TLEE1132 pKa = 4.52 SINMWQSNKK1141 pKa = 8.38 DD1142 pKa = 3.08 TKK1144 pKa = 11.07 LYY1146 pKa = 10.5 FKK1148 pKa = 10.74 SPYY1151 pKa = 10.31 QCMTDD1156 pKa = 3.3 TFSQTLDD1163 pKa = 3.3 CALEE1167 pKa = 4.06 KK1168 pKa = 10.62 FSAEE1172 pKa = 4.05 FYY1174 pKa = 9.77 ISVFIVPMFLIIYY1187 pKa = 8.45 FIMLICCACVKK1198 pKa = 10.36 KK1199 pKa = 10.57 SYY1201 pKa = 10.11 RR1202 pKa = 11.84 YY1203 pKa = 9.72 KK1204 pKa = 10.95 KK1205 pKa = 10.27 LGNSAKK1211 pKa = 10.27 KK1212 pKa = 10.39 GRR1214 pKa = 11.84 WLIADD1219 pKa = 3.56 MADD1222 pKa = 3.32 NIMKK1226 pKa = 9.93 GKK1228 pKa = 8.85 PKK1230 pKa = 10.32 FWIRR1234 pKa = 11.84 PDD1236 pKa = 3.77 NSYY1239 pKa = 10.84 EE1240 pKa = 3.81 WDD1242 pKa = 4.08 DD1243 pKa = 4.41 NDD1245 pKa = 3.17 IGPYY1249 pKa = 10.01 KK1250 pKa = 10.01 RR1251 pKa = 11.84 TTTGRR1256 pKa = 11.84 FRR1258 pKa = 11.84 YY1259 pKa = 9.51 NKK1261 pKa = 9.71 LKK1263 pKa = 10.57 AWSNEE1268 pKa = 3.13 LDD1270 pKa = 3.29
Molecular weight: 143.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.196
IPC2_protein 6.415
IPC_protein 6.389
Toseland 6.351
ProMoST 6.781
Dawson 6.868
Bjellqvist 6.956
Wikipedia 6.839
Rodwell 6.868
Grimsley 6.402
Solomon 6.868
Lehninger 6.868
Nozaki 7.249
DTASelect 7.205
Thurlkill 7.234
EMBOSS 7.22
Sillero 7.307
Patrickios 3.897
IPC_peptide 6.883
IPC2_peptide 6.795
IPC2.peptide.svr19 6.773
Protein with the highest isoelectric point:
>tr|A0A1L4AB05|A0A1L4AB05_9VIRU Nucleocapsid protein OS=Sanxia Water Strider Virus 2 OX=1608061 GN=N PE=4 SV=1
MM1 pKa = 7.07 STTIKK6 pKa = 10.49 SEE8 pKa = 3.7 QFTNVQQVVIPDD20 pKa = 3.26 KK21 pKa = 10.61 WADD24 pKa = 3.48 KK25 pKa = 10.29 LASEE29 pKa = 4.99 SEE31 pKa = 4.34 VTYY34 pKa = 9.74 STNKK38 pKa = 10.11 LEE40 pKa = 4.23 VYY42 pKa = 9.82 QVIFKK47 pKa = 11.03 KK48 pKa = 10.75 LMTRR52 pKa = 11.84 VPVIGSDD59 pKa = 3.53 MEE61 pKa = 4.33 VDD63 pKa = 4.45 LDD65 pKa = 4.14 TPPAAYY71 pKa = 10.15 AFATYY76 pKa = 8.46 VTSDD80 pKa = 3.43 FYY82 pKa = 11.32 RR83 pKa = 11.84 DD84 pKa = 3.55 QVSKK88 pKa = 10.08 STGEE92 pKa = 3.77 VKK94 pKa = 10.84 YY95 pKa = 10.46 LLKK98 pKa = 10.74 VGQQEE103 pKa = 3.95 IPVYY107 pKa = 8.11 STRR110 pKa = 11.84 KK111 pKa = 9.22 SAQKK115 pKa = 10.69 LDD117 pKa = 4.13 DD118 pKa = 4.13 GLISVDD124 pKa = 3.98 DD125 pKa = 3.91 ATLASIMLQNTVVKK139 pKa = 10.19 QFKK142 pKa = 9.03 EE143 pKa = 4.39 KK144 pKa = 10.28 KK145 pKa = 9.03 SAWAMTPLSRR155 pKa = 11.84 ISMTNEE161 pKa = 3.94 GIEE164 pKa = 4.02 ALADD168 pKa = 3.53 EE169 pKa = 5.16 MKK171 pKa = 10.97 VSDD174 pKa = 3.63 TTIVKK179 pKa = 8.59 MLNMSTARR187 pKa = 11.84 KK188 pKa = 9.69 SYY190 pKa = 9.63 QCSDD194 pKa = 3.76 IIGSGYY200 pKa = 10.75 SSDD203 pKa = 3.65 FAVASILRR211 pKa = 11.84 SNFLNDD217 pKa = 3.42 RR218 pKa = 11.84 NKK220 pKa = 9.19 VTMLQKK226 pKa = 10.59 QISDD230 pKa = 3.67 KK231 pKa = 11.27 NIARR235 pKa = 11.84 CVRR238 pKa = 11.84 AAPIDD243 pKa = 3.77 KK244 pKa = 10.66 DD245 pKa = 3.6 RR246 pKa = 11.84 VAMILSCMSGISNDD260 pKa = 3.17 VDD262 pKa = 3.59 KK263 pKa = 11.67 EE264 pKa = 4.15 EE265 pKa = 5.0 ILMKK269 pKa = 10.6 VKK271 pKa = 10.21 NLQSIGVKK279 pKa = 9.86 RR280 pKa = 11.84 RR281 pKa = 11.84 AGLFEE286 pKa = 4.38 EE287 pKa = 4.51 HH288 pKa = 6.08 TKK290 pKa = 10.53 AHH292 pKa = 5.69 SVEE295 pKa = 4.17 NFPTEE300 pKa = 4.27 AEE302 pKa = 4.12 TLSYY306 pKa = 11.21 LEE308 pKa = 4.38 ARR310 pKa = 11.84 GIQDD314 pKa = 3.23 RR315 pKa = 11.84 QNLKK319 pKa = 10.73 KK320 pKa = 10.01 IVKK323 pKa = 8.93 EE324 pKa = 4.08 VKK326 pKa = 10.01 LNKK329 pKa = 10.18 ALTKK333 pKa = 9.26 STKK336 pKa = 8.37 EE337 pKa = 3.64 QILNKK342 pKa = 9.8 IAEE345 pKa = 4.33 KK346 pKa = 10.6 LAA348 pKa = 3.9
Molecular weight: 38.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.226
IPC2_protein 8.185
IPC_protein 8.097
Toseland 8.858
ProMoST 8.653
Dawson 9.136
Bjellqvist 8.931
Wikipedia 9.238
Rodwell 9.428
Grimsley 9.121
Solomon 9.224
Lehninger 9.209
Nozaki 9.107
DTASelect 8.858
Thurlkill 9.033
EMBOSS 9.311
Sillero 9.165
Patrickios 4.94
IPC_peptide 9.224
IPC2_peptide 7.439
IPC2.peptide.svr19 7.634
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3962
348
2344
1320.7
151.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.619 ± 0.586
1.489 ± 0.513
6.663 ± 0.269
6.032 ± 0.58
4.417 ± 0.345
4.619 ± 0.411
1.212 ± 0.119
8.253 ± 0.259
8.657 ± 0.358
8.758 ± 0.377
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.877 ± 0.163
7.774 ± 0.656
2.524 ± 0.273
3.231 ± 0.395
4.064 ± 0.6
7.496 ± 0.671
5.906 ± 0.4
5.704 ± 0.516
0.883 ± 0.265
4.821 ± 0.44
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here